Diabetes Models Phenotyping

糖尿病模型表型分析

基本信息

批准号：
10583248
负责人：
Irfan J Lodhi
金额：
$ 13.34万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-12-01 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10583248
关键词：
Affect Animal Model Animals Biochemical Biological Assay Blood pressure determination Body Composition Cell Culture Techniques Cells Cholesterol Clinical Communities Consultations Data Diabetes Mellitus Disease Dual-Energy X-Ray Absorptiometry Energy Metabolism Environment Experimental Designs Feedback Funding Glucose Glucose Clamp Goals Human Hyperinsulinism In Vitro Indirect Calorimetry Laboratories Measures Metabolic Metabolic Diseases Metabolism Methodology Modeling Modification Molecular Mus Nonesterified Fatty Acids Obesity Organ Organism Paper Patient Care Phenotype Productivity Publications Reproducibility Research Research Personnel Research Project Grants Sampling Scanning Scientist Serum Services Surveys Systemic disease Technology Testing Time Tissues Training Translating Triglycerides United States National Institutes of Health Universities Washington clinical application cost effective cost effectiveness diabetic experience experimental study glucose tolerance improved innovation insight instrumentation insulin tolerance meetings member mouse model novel response service utilization translational potential

项目摘要

PROJECT SUMMARY: Diabetes Models Phenotyping Core The Diabetes Models Phenotyping (DMP) Core is an important component of the Washington University Diabetes Research Center (DRC). The long-term goal of the DMP Core is to enhance diabetes- related research at Washington University using sophisticated services and technologies with the potential to contribute to discoveries that can be translated to novel treatments for diabetes and related disorders. The DMP Core has three objectives: 1) To provide phenotyping services to DRC members to both facilitate and enhance the cost-effectiveness of NIH-funded diabetes/metabolism-related research; 2) To provide expert consultation and training to DRC members in experimental design and characterization of mouse models relevant to diabetes, obesity and associated metabolic diseases; and 3) To develop new research capabilities to enhance the ability of DRC members to perform diabetes and metabolic research. The Core offers a broad range of animal phenotyping services, including a) body composition analysis using EchoMRI and/or DEXA scan; b) indirect calorimetry to measure energy expenditure; c) glucose and insulin tolerance testing; d) blood pressure determinations; and e) biochemical analyses of serum and tissues to measure glucose, triglycerides, free fatty acids and cholesterol. Meeting a key need in the diabetes research community at Washington University, the Core provided services to 44 different DRC member laboratories during the past funding period, including 11 P&F recipients. The services utilized by these investigators over this time frame include biochemical assays of serum (>4,800 samples) and tissues (>1,100 samples), body composition determinations for >8500 mice, and indirect calorimetry assessments in >2,400 mice, to mention the services in greatest demand. These analyses supported the publication of 130 papers. The Core continually evolves in response to user needs through elimination of some services and addition of others. New and expanded services include hyperinsulinemic-euglycemic clamps, thermogenic phenotyping, and expanded training and consultation. Together, DMP Core services continue to help DRC investigators contribute important scholarly advances with substantial potential for translation to improved care for patients suffering from diabetes and related complications.

项目摘要：糖尿病模型表型核心糖尿病模型表型分析 (DMP) 核心是华盛顿研究中心的重要组成部分大学糖尿病研究中心（DRC）。 DMP Core 的长期目标是增强糖尿病- 华盛顿大学的相关研究使用先进的服务和技术，有潜力为可转化为糖尿病及相关疾病的新疗法的发现做出贡献。这 DMP Core 具有三个目标： 1) 为 DRC 成员提供表型分析服务，以促进提高 NIH 资助的糖尿病/代谢相关研究的成本效益； 2) 提供为 DRC 成员提供实验设计和表征方面的专家咨询和培训与糖尿病、肥胖症和相关代谢疾病相关的小鼠模型； 3）开发新的研究能力，以增强 DRC 成员进行糖尿病和代谢研究的能力研究。 The Core 提供广泛的动物表型分析服务，包括 a) 身体成分使用 EchoMRI 和/或 DEXA 扫描进行分析； b) 间接量热法测量能量消耗； c) 葡萄糖和胰岛素耐受性测试； d) 血压测定； e) 血清生化分析和组织来测量葡萄糖、甘油三酯、游离脂肪酸和胆固醇。满足糖尿病的关键需求华盛顿大学研究社区，核心为 44 名不同的 DRC 成员提供服务过去资助期间的实验室，包括 11 个 P&F 获奖者。这些使用的服务在此时间范围内，研究人员对血清（> 4,800 个样本）和组织（> 1,100 个样本）进行了生化分析。样品）、> 8500 只小鼠的身体成分测定以及 > 2,400 只小鼠的间接量热评估老鼠，更不用说需求最大的服务了。这些分析支持了 130 篇论文的发表。核心不断发展，通过消除某些服务并添加某些服务来响应用户需求其他的。新的和扩展的服务包括高胰岛素-正常血糖钳夹、生热表型分析、并扩大培训和咨询。 DMP Core 服务共同继续帮助 DRC 调查人员贡献重要的学术进步，具有转化为改善患者护理的巨大潜力患有糖尿病和相关并发症。