OPTICAL BIOSENSOR FOR THE EARLY DETECTION OF BREAST CANCER

用于乳腺癌早期检测的光学生物传感器

• 批准号: 7603863
• 负责人: John Ernest Shively
• 金额: $ 0.19万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7603863
• 关键词: Antibodies; Biological Assay; Biological Models; Biopsy; Biosensor; Blood; Breast; Breast Cancer Early Detection; Carcinoembryonic Antigen; Computer Retrieval of Information on Scientific Projects Database; Detection; Devices; Funding; Grant; Institution; Label; Measurement; Nipples; Optics; Paper; Patients; Proteins; Purpose; Pyroxylin; Research; Research Personnel; Resources; Sampling; Sensitivity and Specificity; Serum; Services; Source; Staging; Testing; Tumor Markers; United States National Institutes of Health; malignant breast neoplasm

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the first stage we propose to determine if the measurement of proteins (specifically CEA) secreted by the breast captured in a nitrocellulose filter can be used in detecting breast cancer. For that purpose we will obtain samples from nipple secretions in nitrocellulose paper from patients requiring a breast biopsy to determine the sensitivity and specificity of the assay. In a second stage an optical biosensor that has been optimized for the detection of CEA will be used to detect flourescent labeled antibodies directed against CEA found in the serum and breast secretions. The tumor marker carcinoembryonic antigen (CEA) will be used as an early marker for breast cancer in blood and nipple secretions as a model system. Ultimately, the biosensor will be a handheld device that can be used to test minute amounts of blood or secretions in a point of service context. The availability of such a device should allow earlier and more convenient testing for CEA and, eventually, for other tumor markers.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 在第一阶段，我们建议确定硝酸纤维素过滤器捕获的乳房分泌的蛋白质（特别是 CEA）的测量是否可用于检测乳腺癌。 为此，我们将从需要乳房活检的患者的硝化纤维纸上采集乳头分泌物样本，以确定检测的敏感性和特异性。 在第二阶段，针对 CEA 检测进行了优化的光学生物传感器将用于检测针对血清和乳腺分泌物中发现的 CEA 的荧光标记抗体。 肿瘤标志物癌胚抗原（CEA）将作为血液和乳头分泌物中乳腺癌的早期标志物作为模型系统。 最终，生物传感器将成为一种手持设备，可用于在服务点环境中测试微量血液或分泌物。 这种设备的出现应该可以更早、更方便地检测 CEA，并最终检测其他肿瘤标志物。