IMMUNOLOGICAL AND GENETIC ANALYSIS OF AUTOINFLAMMATORY GENES IN FIBROMYALGIA

纤维肌痛自身炎症基因的免疫学和遗传学分析

• 批准号: 7368180
• 负责人: John Ernest Shively
• 金额: $ 3.05万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7368180
• 关键词: IMMUNOLOGICAL; GENETIC; ANALYSIS; AUTOINFLAMMATORY; GENES

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Purpose: to determine if patients with fibromyalgia have hyperactive neutrophils and/or carry genetic polymorphisms in newly discovered autoinflammatory genes (pyringene family). Assessment: (1) Compare response of patient neutrophils vs controls to various stimuli in migration, invasion, and cytokine release assays. (2) Sequence exons of five autoinflammatory genes and determine if polymorphisms are shared among patients and their parents vs control. Significance: this is a common syndrome (affecting 2 to 6% of the US population) predominantly in females (85%) that has no known cause or standard treatment. The diagnosis is made on tender point examination. If we can demonstrate that polymorphisms in autoinflammatory genes are causative and that neutrophil assays can diagnose the disease, new treatments an diagnostic procedures could be devised.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。目的：确定纤维肌痛患者是否患有中性粒细胞过度活跃和/或携带新发现的自身炎症基因（芘烯家族）的遗传多态性。 评估：(1) 在迁移、侵袭和细胞因子释放测定中比较患者中性粒细胞与对照对各种刺激的反应。 (2) 对五个自身炎症基因的外显子进行测序，并确定患者及其父母与对照之间是否共享多态性。意义：这是一种常见的综合征（影响 2% 至 6% 的美国人口），主要发生在女性 (85%) 中，病因不明，也没有标准治疗方法。诊断是通过压痛点检查做出的。如果我们能够证明自身炎症基因的多态性是致病因素，并且中性粒细胞检测可以诊断这种疾病，那么就可以设计出新的治疗方法和诊断程序。