Sequencing and Epigenetics Core

测序和表观遗传学核心

• 批准号: 10580760
• 负责人: Gregory Seumois
• 金额: $ 18.7万
• 依托单位: LA JOLLA INSTITUTE FOR IMMUNOLOGY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-11 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10580760
• 关键词: Sequencing; Epigenetics; Core

Sequencing and Epigenetics Core (Core C) Core Summary The proposed projects in this CCHI program will use genetic approaches to define comprehensive molecular properties of specific immune cells in different aspects of immune responses in different diseases and after vaccination from blood and human clinical tissues (lymph nodes and lung tissue). Facing the inherent challenge, of cell abundance and heterogeneity when working with human samples, the CCHI Sequencing and Epigenetics Core, unlike any other external facility, will provide a unique and comprehensive set of procedures especially validated for isolating and processing small numbers of immune cell populations and support downstream single-cell and bulk transcriptomic as well as epigenetic studies. First, the centralized team of the Sequencing Core will provide assistance to all CCHI project users for careful collection of small number of immune cells, adequate processing and storage of samples for all transcriptomic and epigenetic assays listed in the proposal. Second, the Sequencing Core will perform all micro-scaled bulk RNA-seq as well as single-cell RNA sequencing and will provide support for the whole transcriptome analysis, TCR and BCR highly variable sequence extraction from bulk and single-cell RNA-seq datasets. Finally, the Sequencing Core will perform micro-scaled library preparation for epigenetic assays (ATAC- and ChIP-Seq).

测序和表观遗传学核心（核心 C） 核心总结 CCHI 计划中的拟议项目将使用遗传方法来定义全面的分子 特定免疫细胞在不同疾病及治疗后免疫反应不同方面的特性 从血液和人体临床组织（淋巴结和肺组织）进行疫苗接种。面对固有的挑战， 处理人类样本时的细胞丰度和异质性、CCHI 测序和表观遗传学 与任何其他外部设施不同，核心将提供一套独特且全面的程序，尤其是 经验证可用于分离和处理少量免疫细胞群，并支持下游单细胞和批量转录组以及表观遗传学研究。首先，测序核心的集中团队将 为所有 CCHI 项目用户提供帮助，以仔细收集少量、足够的免疫细胞 处理和储存提案中列出的所有转录组和表观遗传学测定的样品。第二， 测序核心将执行所有微型批量 RNA 测序以及单细胞 RNA 测序，并将 为全转录组分析、TCR 和 BCR 高变异序列提取提供支持 批量和单细胞 RNA-seq 数据集。最后，测序核心将进行微型文库制备 用于表观遗传学分析（ATAC-和 ChIP-Seq）。