Supplement application for a CLARIOstar Plus microplate reader with six detection modalities

具有六种检测模式的 CLARIOstar Plus 酶标仪的补充应用

• 批准号: 10577405
• 负责人: RAJAT ROHATGI
• 金额: $ 10.88万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10577405
• 关键词: Administrative Supplement; Award; Biochemistry; Biological Assay; Cell Communication; Cell Nucleus; Cells; Cellular Assay; Degenerative Disorder; Detection; Erinaceidae; Fluorescence; Fluorescence Polarization; Funding; Gene Activation; Goals; Kinetics; Laboratories; Maintenance; Malignant Neoplasms; Measurement; Modality; Monitor; National Institute of General Medical Sciences; Pathway interactions; Performance; Proteins; Reader; Research; Research Project Grants; Services; Signal Transduction; Speed; System; Time; Tissues; Training; WNT Signaling Pathway; Work; cost effective; design; developmental disease; instrument; luminescence; programs; repaired; small molecule; smoothened signaling pathway

Supplement Summary Supplement application for a CLARIOstar Plus microplate reader with six detection modalities This is an administrative supplement application for the purchase of a CLARIOstar Plus multi-mode microplate reader from BMG Labtech. The CLARIOStar is a high-performance, medium-throughput instrument with environmental control that provides a unifying solution to the six detection modalities used routinely in our NIGMS-funded work: UV/Vis absorbance, fluorescence, luminescence, fluorescence polarization, time-resolved fluorescence and ALPHA assays. I am requesting the CLARIOStar for the following reasons. (1) It will replace a broken, decade-old microplate reader that is used in my laboratory for Hedgehog and WNT signaling assays central to all the projects supported by my NIGMS MIRA (R35 GM118082) award. (2) It will advance the goals of the research program outlined in my 2021 NIGMS R35/MIRA renewal by enhancing our capability to perform live cell assays with environmental control, protein-protein and small molecule-protein interaction measurements, and kinetic measurements, all with ten-fold higher speed, sensitivity and dynamic range compared to our previous instrument. (3) A microplate reader with these capabilities is not presently available at Stanford on a cost-effective basis given our intensive usage needs. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the microplate reader. In summary, the CLARIOstar will accelerate NIGMS-funded research projects in my laboratory and provide cutting-edge training in assay design and optimization for trainees that work on these projects.

补充摘要 具有六种检测模式的 CLARIOstar Plus 酶标仪的补充应用 这是购买 CLARIOstar Plus 多模式微孔板的行政补充申请 BMG Labtech 的读者。 CLARIOStar 是一款高性能、中等通量的仪器 环境控制为我们日常使用的六种检测方式提供了统一的解决方案 NIGMS 资助的工作：紫外/可见光吸光度、荧光、发光、荧光偏振、 时间分辨荧光和 ALPHA 测定。我出于以下原因请求 CLARIOStar。 (1) 它将取代我实验室用于 Hedgehog 和 WNT 的一台已损坏、已有十年历史的酶标仪 信号分析是我的 NIGMS MIRA (R35 GM118082) 奖项支持的所有项目的核心。 (2) 将会 通过增强我们的能力，推进我的 2021 年 NIGMS R35/MIRA 更新中概述的研究计划的目标 能够通过环境控制、蛋白质-蛋白质和小分子-蛋白质进行活细胞测定 相互作用测量和动力学测量，速度、灵敏度和动态均提高十倍 与我们之前的仪器相比的范围。 (3) 目前还没有具有这些功能的酶标仪 考虑到我们的密集使用需求，斯坦福大学可以以经济高效的方式提供。生物化学系 斯坦福大学将通过仪器服务资金和容纳微孔板的空间来支持此次购买 读者。总之，CLARIOstar 将加速我实验室和 NIGMS 资助的研究项目 为从事这些项目的学员提供检测设计和优化方面的前沿培训。