Administrative supplement application for equipment purchase

设备采购行政补充申请

• 批准号: 10795312
• 负责人: RAJAT ROHATGI
• 金额: $ 17.44万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10795312
• 关键词: Acceleration; Administrative Supplement; Affinity Chromatography; Automation; Award; Biochemistry; Cell Communication; Cell Nucleus; Cells; Chromatography; Computer software; Core Facility; Degenerative Disorder; Documentation; Erinaceidae; Funding; Gel Chromatography; Gene Activation; Hour; Hydrophobic Interactions; Ion Exchange; Laboratories; Liquid Chromatography; Maintenance; Malignant Neoplasms; Monitor; National Institute of General Medical Sciences; Operating System; Pathway interactions; Protein Analysis; Proteins; Reproducibility; Resolution; Running; Services; Signal Transduction; System; Time; Tissues; Training; Vendor; cost effective; developmental disease; equipment acquisition; improved; instrument; pressure; protein purification; repaired; skills

Supplement Summary Administrative supplement application for equipment purchase This is an administrative supplement application for the purchase of a AKTA_Pure Fast Pressure Liquid Chromatography (FPLC) system from Cytiva. The AKTA_Pure FPLC is a medium flow, medium pressure chromatography system that allows the semi-automated, rapid, and high-resolution chromatographic separation of proteins by ion-exchange, hydrophobic interaction, gel-filtration, and affinity chromatography. Protein purification and analysis, enabled by the FPLC, is a central and indispensable requirement for all the projects being pursued in my laboratory as part of my NIGMS-funded R35/MIRA award. The new AKTA_Pure FPLC being requested in this supplement will directly replace a malfunctioning AKTA_Explorer instrument that I purchased 13 years ago (in 2009) when I first started my laboratory at Stanford and that is no longer serviceable. We provide documentation from the current vendor (Cytiva) that support for this old AKTA_Explorer system lapsed in 2018. Parts are not available, vendor support is not available and the operating system required to run instrument software is not supported by either Microsoft or Cytiva. Our intensive use (~20-30 hours per week) and project-specific configurations preclude the cost-effective and time-efficient use of chromatography systems in core facilities at Stanford. Finally, the improved automation and software that comes with the new system will dramatically accelerate our workflows, improve reproducibility and provide up-to-date training in the core skills of protein purification and analysis for trainees working on our NIGMS-funded projects. The Biochemistry Department at Stanford will support this purchase with both funds for instrument service and space to house the AKTA_Pure FPLC system.

补充摘要 设备采购行政补充申请 这是购买 AKTA_Pure 快速压力液体的行政补充申请 Cytiva 的色谱 (FPLC) 系统。 AKTA_Pure FPLC 是一款中等流量、中等压力的 色谱系统可实现半自动、快速、高分辨率的色谱分析 通过离子交换、疏水相互作用、凝胶过滤和亲和层析分离蛋白质。 由 FPLC 实现的蛋白质纯化和分析是所有分析的核心且不可或缺的要求。 我的实验室正在开展的项目是 NIGMS 资助的 R35/MIRA 奖的一部分。新的 AKTA_Pure 本补充中要求的 FPLC 将直接替换出现故障的 AKTA_Explorer 仪器，该仪器 13 年前（2009 年）当我第一次在斯坦福大学开始我的实验室时，我购买了它，但现在已经不再是这样了。 可用。我们提供当前供应商 (Cytiva) 提供的支持此旧版本的文档 AKTA_Explorer 系统于 2018 年失效。零件不可用，供应商支持不可用，并且 Microsoft 或 Cytiva 均不支持运行仪器软件所需的操作系统。我们的 密集使用（每周约 20-30 小时）和特定于项目的配置排除了成本效益和 在斯坦福大学的核心设施中高效利用色谱系统。最后，改进的自动化 新系统附带的软件将极大地加快我们的工作流程，改善 重现性，并为学员提供蛋白质纯化和分析核心技能的最新培训 致力于我们的 NIGMS 资助的项目。斯坦福大学生物化学系将支持此次购买 拥有仪器服务资金和容纳 AKTA_Pure FPLC 系统的空间。

项目成果

Direct ionic stress sensing and mitigation by the transcription factor NFAT5.

通过转录因子 NFAT5 直接感应和缓解离子应力。

• DOI: 10.1101/2023.09.23.559074
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Khandwala,ChandniB;Sarkar,Parijat;Schmidt,HBroder;Ma,Mengxiao;Kinnebrew,Maia;Pusapati,GaneshV;Patel,BhavenB;Tillo,Desiree;Lebensohn,AndresM;Rohatgi,Rajat
• 通讯作者: Rohatgi,Rajat

CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog.

• DOI: 10.1016/j.devcel.2017.12.003
• 发表时间: 2018-01-08
• 期刊: Developmental cell
• 影响因子: 11.8
• 作者: Pusapati GV;Kong JH;Patel BB;Krishnan A;Sagner A;Kinnebrew M;Briscoe J;Aravind L;Rohatgi R
• 通讯作者: Rohatgi R

Bile acid biosynthesis in Smith-Lemli-Opitz syndrome bypassing cholesterol: Potential importance of pathway intermediates.

• DOI: 10.1016/j.jsbmb.2020.105794
• 发表时间: 2021-03
• 期刊: The Journal of steroid biochemistry and molecular biology
• 作者: Abdel-Khalik J;Hearn T;Dickson AL;Crick PJ;Yutuc E;Austin-Muttitt K;Bigger BW;Morris AA;Shackleton CH;Clayton PT;Iida T;Sircar R;Rohatgi R;Marschall HU;Sjövall J;Björkhem I;Mullins JGL;Griffiths WJ;Wang Y
• 通讯作者: Wang Y

The Energetics and Ion Coupling of Cholesterol Transport Through Patched1

• DOI: 10.1101/2023.02.14.528445
• 发表时间: 2023-02
• 期刊: bioRxiv
• 作者: T. Ansell;R. Corey;Lucrezia Vittoria Viti;M. Kinnebrew;R. Rohatgi;C. Siebold;M. Sansom

R-spondins can potentiate WNT signaling without LGRs.

• DOI: 10.7554/elife.33126
• 发表时间: 2018-02-06
• 期刊: eLife
• 影响因子: 7.7
• 作者: Lebensohn AM;Rohatgi R
• 通讯作者: Rohatgi R