TAFopathies Result from Derangements in Transcriptional Control of Metabolism

TAF 病是由代谢转录控制紊乱引起的

基本信息

批准号：
10579824
负责人：
Jamison Leid
金额：
$ 3.27万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10579824
关键词：
3-Dimensional Adaptive Behaviors Affect Anatomy Basal metabolic rate Binding Proteins Biological Assay Bromodomain CRISPR/Cas technology Cardiac Cardiac development Cell Differentiation process Cell Respiration ChIP-seq Clinical Complex Craniofacial Abnormalities Data Data Analyses Defect Development Disease Embryo Embryonic Development Enhancers Exhibits Experimental Designs Experimental Models Fertilization Gene Expression General Population Genes Genetic Genetic Enhancer Element Genetic Screening Genetic Transcription Goals Heart Heart Abnormalities Heart failure Hi-C Histology Hour Human In Situ Hybridization Individual Intellectual functioning disability Interruption Lead Learning Link Mammals Mediating Metabolic Metabolic Activation Metabolic dysfunction Metabolism Mitochondria Modeling Mutation Neurodevelopmental Disorder Neurologic Nonsense Mutation Pathogenicity Patients Pattern Phenotype Proteins RNA RNA Polymerase II Regulation Respiration Role Signal Transduction Site Structural defect Syndrome TAF1 gene TAF2 gene TAF5 gene TAF6 gene TATA-Binding Protein Associated Factors TATA-Box Binding Protein Techniques Testing Time Transcriptional Activation Transcriptional Regulation United States Variant Ventricular X ray microscopy Zebrafish chromatin immunoprecipitation cognitive function computational pipelines congenital heart disorder craniofacial craniofacial development experimental study innovation loss of function mutation mutant neurodevelopment novel patient subsets programs promoter recruit skills spatiotemporal transcription factor transcriptome sequencing treatment strategy

项目摘要

Project Summary Intellectual disability is a disabling neurodevelopmental disorder that affects 2-3% of the general population. Often times, these intellectual disabilities are accompanied by additional developmental abnormalities. These syndromic forms of intellectual disability frequently have a genetic basis. Recent studies have identified a subset of intellectual disability patients with pathogenic variants in key components of the TATA-binding protein associated factors (TAFs). These patients also have craniofacial defects and congenital heart disease. This syndrome has been termed as a TAFopathy and includes mutations in TATA binding protein (TBP), TAF1, TAF2, and TAF6. Through a forward genetic screen, we have recovered a lethal nonsense mutation in TAF5. Mutant embryos have craniofacial hypoplasia, ventricular hypoplasia, and heart failure at 96 hours post fertilization. CRISPR/Cas9 mediated gene editing revealed that this phenotype was recapitulated in TAF1 KO and TAF5 KO embryos. TAF5 KOs show significant metabolic dysfunction that may precede the anatomical defects observed at 96 hours post fertilization. Together, these findings support a regulatory role for TAFs in coordinating transcriptional activation of metabolic programming during embryogenesis. The TAFs form the general transcription factor TFIID, which recruits RNA Polymerase II to form the preinitation complex at sites of transcription. Studies have shown that individual TAFs are not necessary for general transcription. Other studies have identified functional domains of TAF1 that activate transcription through interactions with TBP and enhancer elements that are sufficient to recruit RNA Polymerase II to sites of transcription. In conjunction with our data showing metabolic dysfunction, we hypothesize that TAFs regulate metabolic programs by linking enhancers and promoters of metabolic genes through TFIID assembly. Our current efforts are focused towards completing our phenotypic characterization of TAF1 KOs and TAF5 KOs with respect to craniofacial- and neuro-development. We plan to use innovative and cutting-edge techniques, including x-ray microscopy, RNA- Scope in situ hybridization, mitochondrial respiration assays, ChIP-seq, and Hi-C to achieve this goal. The data obtained will help us elucidate basic disease mechanisms of TAFopathy. These findings would also indicate a secondary function for general transcription factors to regulate metabolic programming during embryogenesis. .

项目摘要智力残疾是一种残疾神经发育障碍，影响了总人群的2-3％。这些智力残疾通常伴随着其他发育异常。这些智力残疾的综合征形式经常具有遗传基础。最近的研究确定了在塔塔结合蛋白的关键成分中具有致病变异的智力残疾患者的子集相关因素（TAF）。这些患者还患有颅面缺陷和先天性心脏病。这综合征称为tafopathy，包括TATA结合蛋白（TBP），TAF1， TAF2和TAF6。通过正向遗传筛选，我们在TAF5中恢复了致命的废话突变。突变胚胎患有颅面性发育不全，室性发育不全和心力衰竭后96小时受精。 CRISPR/CAS9介导的基因编辑表明，TAF1 KO中概括了这种表型和TAF5 KO胚胎。 Taf5 KOS显示出可能在解剖学之前的代谢功能障碍受精后96小时观察到的缺陷。这些发现共同支持TAF中的调节作用胚胎发生过程中代谢编程的转录激活。 TAFS形成一般转录因子TFIID，募集RNA聚合酶II以在转录。研究表明，单个TAF是一般转录所必需的。其他研究已经确定了TAF1的功能域，这些域通过与TBP和TBP和足以募集RNA聚合酶II到转录位点的增强子元素。结合我们显示代谢功能障碍的数据，我们假设TAF通过链接来调节代谢程序通过TFIID组装的代谢基因的增强子和启动子。我们目前的努力专注于完成TAF1 KOS和TAF5 KO的表型表征相对于颅面和神经发展。我们计划使用创新和尖端技术，包括X射线显微镜，RNA- 原位杂交，线粒体呼吸测定，芯片序列和HI-C以实现这一目标。数据获得的将有助于我们阐明tafopathy的基本疾病机制。这些发现也表明一般转录因子的次要功能，以调节胚胎发生过程中代谢编程。。