Molecular Mechanisms

分子机制

• 批准号: 10569515
• 负责人: J Michael Salbaum
• 金额: $ 52.92万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10569515
• 关键词: 3-Dimensional; Affect; Applications Grants; Biological; Biomedical Research; Biomedical Technology; Cells; Centers of Research Excellence; Complex; Computer software; Data; Data Collection; Data Set; Disease; Ensure; Environment; Equipment; Etiology; Evaluation; Experimental Designs; Expression Profiling; Funding; Future; Gene Expression; Generations; Genomic approach; Genomics; Goals; Histology; Human Resources; Individual; Informatics; Knowledge; Laboratories; Leadership; Light; Maintenance; Measurement; Mentors; Metabolic; Methods; Microscopy; Modernization; Molecular; Outcome; Ownership; Pathogenicity; Principal Investigator; Productivity; RNA; Reproducibility; Research; Research Personnel; Research Project Grants; Resources; Running; Science; Secure; Solid; Systems Biology; Technical Expertise; Technology; Three-Dimensional Imaging; Tissues; United States National Institutes of Health; Visualization; bioimaging; career; cost; design; experimental study; functional genomics; gene product; imaging approach; imaging modality; insight; instrumentation; new technology; protocol development; single-cell RNA sequencing; skills; sound; success

Project Summary: Modern biomedical research to uncover the metabolic basis of disease is increasingly dependent on the application of complex technology. In many cases, the costs associated with the acquisition and maintenance of necessary equipment, in addition to the technical expertise needed to run these technologies, typically exceed the capabilities of individual laboratories - particularly those of investigators at the start of their careers. Hence, the overarching goal for the COBRE Molecular Mechanisms Core is to provide investigators with such technologies in the realm of functional genomics and molecular bioimaging, and thus help them to apply state of the art approaches in their research projects. The Core seeks to achieve this goal in two Specific Aims: SPECIFIC AIM 1: To provide COBRE investigators with mentoring support concerning molecular bioimaging and functional genomics technology. Application of modern biomedical technology per se does not lead to meaningful or impactful scientific progress; it requires a clear understanding of the power of specific technologies for science to emerge from data. Therefore, the Core will serve as an intellectual and advisory resource for COBRE investigators when it comes to bioimaging and functional genomics experiments, and provide mentoring so that technological approaches match up with the scientific goal of each project. Hence, the goal for this Aim is to ensure that COBRE investigators, rather than viewing technology as a perhaps unsurmountable obstacle, can in fact embrace these new technologies with confidence to further their science. SPECIFIC AIM 2: To provide COBRE investigators with direct technological support for their experiments that use molecular bioimaging and functional genomics approaches. To understand the molecular etiology that forms the pathogenic framework for disease, technologies that capture gene expression changes are of primary necessity for conducting mechanistic research, and the key technologies for the Core are three-dimensional imaging of gene expression in whole-mount tissues, and assessment of gene expression using single-cell RNA sequencing. The Core will provide COBRE investigators with turn-key access to these technologies in order to ensure that they do not get needlessly bogged down in the hands-on technical minutiae, but can fully concentrate on the science of their research projects, and on their overall productivity. Furthermore, the Core, by relying on its NIH proposal review expertise, and by ensuring that experiments meet NIH rigor and reproducibility standards and thus contribute to a strong premise, can provide the mentoring environment needed for COBRE investigators to generate competitive NIH grant proposals.

项目摘要： 现代生物医学研究以发现疾病的代谢基础越来越取决于 复杂技术的应用。在许多情况下，与收购和维护相关的成本 除了运行这些技术所需的技术专长外，通常超过了必要的设备 个别实验室的能力 - 尤其是在职业生涯开始时研究人员的能力。因此， 毛cre分子机制核心的总体目标是为研究人员提供这样的 功能基因组学和分子生物成像领域中的技术，从而帮助他们应用状态 艺术在他们的研究项目中采用。核心试图以两个具体的目的实现这一目标： 特定目的1：向毛病研究人员提供有关分子生物成像和的指导支持 功能基因组技术。现代生物医学技术本身的应用不会导致 有意义或有影响力的科学进步；它需要清楚地了解特定技术的力量 从数据中出现科学。因此，核心将作为智力和咨询资源 在生物成像和功能基因组学实验方面，鞋底研究人员并提供指导 因此，这种技术方法与每个项目的科学目标相吻合。因此，这个目标的目标 是为了确保毛茸茸的调查人员，而不是将技术视为可能毫无疑问的障碍，而是 实际上，可以充满信心地拥抱这些新技术，以进一步发展科学。 特定目的2：为毛茸茸的研究人员为其实验提供直接技术支持， 使用分子生物成像和功能基因组学方法。了解形成的分子病因 疾病的致病框架，捕获基因表达变化的技术是主要的 进行机械研究的必要性，以及核心的关键技术是三维 整个安排组织中基因表达的成像，并使用单细胞RNA评估基因表达 测序。核心将为毛绒调查人员提供对这些技术的旋转访问权限，以便 确保他们不会不必要地陷入动手的技术细节中，而是可以完全集中精力 关于他们的研究项目的科学及其整体生产力。 此外，核心通过依靠其NIH提案审查专业知识，并确保实验相遇 NIH严格和可重复性标准，因此有助于强大的前提，可以提供指导 COBRE调查人员生成竞争性NIH赠款建议所需的环境。