LIGHT-INDUCED DNA BASE EXCITATION, DEACTIVATION AND OXIDATION

光诱导 DNA 碱基激发、失活和氧化

基本信息

批准号：
7959219
负责人：
Ruomei Gao
金额：
$ 12.8万
依托单位：
JACKSON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-06-01 至 2010-05-31
项目状态：
已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. An absolute actinometer is being developed for quantitative characterization of singlet oxygen production upon one- and two-photon excitation for a specific system, e.g., nanosensitizer, DNA bases, etc. Photochemical reactions were carried out under pulsed laser at 532 nm and 355 nm in a cylindrical cell of 5 cm light path length for obtaining efficient photon absorption and 1O2 trapping using DPBF as a singlet oxygen acceptor in highly diluted solutions. A well-established Reincke's salt actinometer was used for the quantitative determination of incident photons at both 532 and 355 nm. FD were determined in chloroform solutions with satisfied accuracy and precision for some phthalocyanines. These compounds were used as standards for testing this method. Key factors that influence 1O2 production through the competitions for both triplet states and singlet oxygen were explored.

该子项目是利用该技术的众多研究子项目之一资源由 NIH/NCRR 资助的中心拨款提供。子项目及研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金，因此可以在其他 CRISP 条目中表示。列出的机构是对于中心来说，它不一定是研究者的机构。正在开发一种绝对光量计，用于定量表征特定系统（例如纳米敏化剂、DNA 碱基等）单线态氧激发时的单线态氧产生。光化学反应在 532 nm 和 355 nm 的脉冲激光下进行。光路长度为 5 cm 的圆柱形池，用于在高度稀释的溶液中使用 DPBF 作为单线态氧受体获得有效的光子吸收和 1O2 捕获。使用完善的 Reincke 盐光化计对 532 和 355 nm 的入射光子进行定量测定。在氯仿溶液中测定某些酞菁的 FD 具有令人满意的准确度和精密度。这些化合物用作测试该方法的标准品。通过三重态和单线态氧的竞争，探讨了影响 1O2 产生的关键因素。