Human Phenotyping Core

人类表型核心

基本信息

批准号：
10251965
负责人：
Joel Marvin Guthridge
金额：
$ 36.85万
依托单位：
OKLAHOMA MEDICAL RESEARCH FOUNDATION
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-07 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10251965
关键词：
Address Autoantibodies Autoimmune Diseases Biological Assay Biological Markers Blood Cells Caring Cells Cellular Indexing of Transcriptomes and Epitopes by Sequencing Characteristics Clinical Competence Complex Consultations Data Data Analyses Data Set Databases Development Diagnostic Disease Effectiveness Ensure Epigenetic Process Equipment Experimental Designs Flow Cytometry Foundations Freezing Funding Future Generations Genetic Genetic Polymorphism Genomic DNA Genomics Genotype Human Human Herpesvirus 4 Human Resources Immune Immunologics Immunophenotyping Individual Inflammatory Investigation Knowledge Measures Mediator of activation protein Methodology Methods Molecular Molecular Profiling Oklahoma Pathogenesis Pathway interactions Patients Phenotype Plasma Population Preparation Process Proteomics Quality Control Reproducibility Research Research Personnel Resources Rheumatism Sampling Serum Services Site Specimen Statistical Models System Technical Expertise Technology Therapeutic Time Transformed Cell Line Variant base biobank chemokine clinical center clinical phenotype clinically relevant computerized data processing cytokine data quality design experience experimental study human disease immune function molecular phenotype novel novel strategies phenotypic data precision medicine prognostic tool programs response single-cell RNA sequencing success transcriptome transcriptomics

项目摘要

Human Phenotyping Core Project Summary The rapid development of new approaches to interrogate the immune and inflammatory systems comes with a growing demand for both technical and conceptual expertise in these methodologies. The Human Phenotyping Core was created to support these activities within the Oklahoma Rheumatic Disease Resources Cores Center (ORDRCC). To address the most broadly scientifically interesting and clinically important questions, our ORDRCC Investigators need access to cutting-edge approaches, such as scRNA-Seq/CITE- Seq, ChipCytometry, and CyTOF to perform incisive experiments and to generate high-quality, reproducible data. The Human Phenotyping Core will allow our investigators to ask novel questions regarding the molecular phenotypes of rheumatic diseases. In addition to providing ORDRCC investigators with high-content biomolecular phenotyping services, the Human Phenotyping Core in conjunction with the Clinical Characterization and Biorepository Core (CCBC) will provide access to datasets containing previously collected genetic, proteomic, and biomolecular phenotypes of clinical subjects whose samples are available from the CCBC. The tight integration of the Human Phenotyping Core, CCBC and our databases will enable investigators to efficiently phenotype samples and correlate molecular and cellular phenotypes with clinical characteristics, to select more homogeneous patients for hypothesis-driven investigation and help provide the foundational knowledge needed for precision medicine in rheumatic disease care. By consolidating a range of technologies required for the complete characterization of molecular or cellular phenotypes into a single unit, the Human Phenotyping Core (HPC) will streamline services to help ORDRCC investigators in the efficient planning, design, execution and data analysis required for their individual projects. The HPC will provide experience in performing research using clinical specimens, technical expertise in a wide range of molecular and bioassays, access to equipment and resources thus enabling cutting-edge approaches to address research questions, quality control assessment of the data collected and data processing suitable for higher end statistical modeling of phenotype/clinical associations. SPECIFIC AIM 1: Assist ORDRCC Investigators, JCIs and Scholars to navigate the stages of project development, experimental design, resource planning and execution of translational and mechanistic studies through Core Management and individual consultations. SPECIFIC AIM 2: Provide access to advanced molecular phenotyping technologies and experienced technical personnel for efficient execution of advanced experimental data plans. SPECIFIC AIM 3: Establish processes to ensure data quality control, processing of raw data, and preparation of data sets for more extensive analysis and statistical modeling. SPECIFIC AIM 4: Evaluate, assess and implement new cutting-edge technologies for molecular phenotyping.

人类表型核心项目摘要质疑免疫和炎症系统的新方法的快速发展是在这些方法中对技术和概念专业知识的需求不断增长。人类创建表型核心是为了支持俄克拉荷马州风湿病资源中的这些活动内核中心（ORDRCC）。解决最广泛的科学有趣和临床上重要的问题，我们的ORDRCC调查人员需要访问尖端方法，例如scrna-seq/cite- SEQ，芯片仪和细胞均进行敏锐的实验并产生高质量的可重复性数据。人类表型核心将使我们的研究人员能够提出有关分子的新问题风湿病的表型。除了为ORDRCC调查人员提供高质量生物分子表型服务，与临床结合的人类表型核心表征和生物座核（CCBC）将提供对包含先前的数据集的访问临床受试者的遗传，蛋白质组学和生物分子表型的样品可用来自CCBC。人类表型核心，CCBC和我们的数据库的紧密整合将启用研究人员有效地表型样品并将分子和细胞表型与临床相关特征，选择更多同质患者进行假设驱动的研究并帮助提供风湿病护理中精确医学所需的基础知识。通过巩固分子或分子的完整表征所需的一系列技术蜂窝表型成一个单元，人类表型核心（HPC）将简化服务以帮助 ORDRCC的调查人员在其高效计划，设计，执行和数据分析中需要个别项目。 HPC将提供使用临床标本进行研究的经验，在各种分子和生物测定的技术专长，因此使用设备和资源的技术专长启用尖端方法来解决研究问题，质量控制数据评估收集和数据处理适用于表型/临床关联的高端统计模型。特定目标1：协助ORDRCC调查人员，JCIS和学者在项目阶段进行导航转化和机械研究的开发，实验设计，资源计划和执行通过核心管理和个人咨询。特定目标2：提供对高级分子表型技术的访问和经验丰富的有效执行高级实验数据计划的技术人员。特定目标3：建立过程以确保数据质量控制，原始数据处理和准备数据集用于更广泛的分析和统计建模。特定目的4：评估，评估和实施用于分子表型的新的尖端技术。