Cardiac Toxicity of Flavorings in Electronic Nicotine Delivery Systems

电子尼古丁输送系统中香料的心脏毒性

基本信息

批准号：
10251155
负责人：
Sami Fouad Noujaim
金额：
$ 44.85万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10251155
关键词：
Action Potentials Adverse effects Affect Aldehydes Animal Model Antioxidants Apoptosis Arrhythmia Blood C57BL/6 Mouse Calcium Cardiac Cardiac Myocytes Cardiac health Cardiotoxicity Cell Culture Techniques Cell Line Cells Characteristics Chemicals Data Electrocardiogram Electrodes Electronic Nicotine Delivery Systems Electrophysiology (science)Exposure to Fibrosis Flavoring Genes Government Heart High School Student Human In Vitro Inhalation Inhalation Exposure Mass Spectrum Analysis Measures Mediating Mitochondria Monitor Mus Muscle Cells Nicotine Optics Oxidative Stress Oxygen Consumption Population Proliferating Public Health Role Smoke Supplementation System Teenagers Telemetry Testing Tobacco Toxic effect Transgenic Mice Translating Ventricular aerosolized age group base catalase cigarette smoking design electronic liquid experimental study extracellular heart rate variability heart rhythm in vivo induced pluripotent stem cell insight overexpression oxidative damage reconstitution tobacco advertising tobacco products vaping vapor young adult

项目摘要

CARDIAC TOXICITY OF FLAVORINGS IN ELECTRONIC NICOTINE DELIVERY SYSTEMS Tobacco cigarette smoking is on the decline, but the usage of electronic nicotine delivery systems (ENDS) is gaining popularity, specifically in the teen and young adult age groups. While the cardiac toxicity of tobacco cigarette smoking has been widely studied and is well established, the possible cardiac toxicity of ENDS products and their design characteristics, such as added flavorings, are largely underexplored. For instance, a form of electronic nicotine delivery known as vaping, uses “e-liquid” in order to generate “e-vapor”, an inhalable smoke-like aerosolized mixture containing nicotine and flavors. Here, we propose to investigate how e-liquids with different flavors affect cardiac in-vitro and in-vivo toxicity, in cell culture and in animal models. Our hypothesis is that inhalation exposure to e-liquid flavorings increases cardiac oxidative stress, leading to electrophysiological toxicity and arrhythmogenesis. We will conduct our studies in three aims: 1)- To investigate the in-vitro cardiac harm and toxicity of e-liquid flavorings, 2)- To investigate the in-vivo cardiac harm and toxicity of e-liquid flavorings, and 3)- To investigate the role of oxidative stress in mediating the cardiac toxicity of e-liquid flavorings. We believe that this proposal will produce new, useful and focused insights into the potential in-vivo and in-vitro adverse effects of e-liquid flavorings using functional and cellular indicators of cardiac harm. Our findings will hopefully be valuable in increasing our understanding of whether the flavoring aspect of ENDS products design could cause cardiac in- vivo and in- vitro toxicities.

电子尼古丁输送系统中调味剂的心脏毒性烟草吸烟正在下降，但是电子尼古丁输送系统（末端）的使用是越来越受欢迎，特别是在青少年和成年年龄段。而烟草的心脏毒性吸烟已广泛研究，并且已经建立了良好的末端心脏毒性产品及其设计特性（例如添加的调味剂）在很大程度上被忽略了。例如，电子尼古丁递送的形式称为vaping，使用“电子液体”来产生“ e-vapor”，一种吸入含有尼古丁和口味的烟雾样混合物。在这里，我们建议研究电子液体如何具有不同的口味会影响心脏内部和体内毒性，细胞培养和动物模型中的毒性。我们的假设是吸入到电子液体风味会增加心脏氧化应激，领先电生理毒性和心律失常。我们将以三个目标进行研究：1） - 研究电子液体口味的体外心脏危害和毒性，2） - 研究体内心脏心脏电子液体调味剂的危害和毒性，以及3） - 研究氧化应激在介导的作用电子液体口味的心脏毒性。我们认为，该建议将产生新的，有用和专注对使用功能和细胞的电子液体调味料的潜在体内和体外不良反应的见解心脏伤害的指标。希望我们的发现将对我们对是否是否理解是否有价值末端产品设计的设计可能会导致心脏体内和体外毒性。