Cardiac Toxicity of Flavorings in Electronic Nicotine Delivery Systems

电子尼古丁输送系统中香料的心脏毒性

基本信息

批准号：
10046578
负责人：
Sami Fouad Noujaim
金额：
$ 42.92万
依托单位：
UNIVERSITY OF SOUTH FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10046578
关键词：
Action Potentials Adverse effects Affect Aldehydes Animal Model Antioxidants Apoptosis Arrhythmia Blood C57BL/6 Mouse Calcium Cardiac Cardiac Myocytes Cardiac health Cardiotoxicity Cell Culture Techniques Cell Line Cells Characteristics Chemicals Data Electrocardiogram Electrodes Electronic Nicotine Delivery Systems Electrophysiology (science)Exposure to Fibrosis Flavoring Genes Government Heart High School Student Human In Vitro Inhalation Inhalation Exposure Mass Spectrum Analysis Measures Mediating Mitochondria Monitor Mus Muscle Cells Nicotine Optics Oxidative Stress Oxygen Consumption Population Proliferating Public Health Role Smoke Supplementation System Teenagers Telemetry Testing Tobacco Toxic effect Transgenic Mice Translating Ventricular aerosolized age group base catalase cigarette smoking design electronic liquid experimental study extracellular heart rate variability heart rhythm in vivo induced pluripotent stem cell insight overexpression oxidative damage reconstitution tobacco advertising tobacco products vaping vapor young adult

项目摘要

CARDIAC TOXICITY OF FLAVORINGS IN ELECTRONIC NICOTINE DELIVERY SYSTEMS Tobacco cigarette smoking is on the decline, but the usage of electronic nicotine delivery systems (ENDS) is gaining popularity, specifically in the teen and young adult age groups. While the cardiac toxicity of tobacco cigarette smoking has been widely studied and is well established, the possible cardiac toxicity of ENDS products and their design characteristics, such as added flavorings, are largely underexplored. For instance, a form of electronic nicotine delivery known as vaping, uses “e-liquid” in order to generate “e-vapor”, an inhalable smoke-like aerosolized mixture containing nicotine and flavors. Here, we propose to investigate how e-liquids with different flavors affect cardiac in-vitro and in-vivo toxicity, in cell culture and in animal models. Our hypothesis is that inhalation exposure to e-liquid flavorings increases cardiac oxidative stress, leading to electrophysiological toxicity and arrhythmogenesis. We will conduct our studies in three aims: 1)- To investigate the in-vitro cardiac harm and toxicity of e-liquid flavorings, 2)- To investigate the in-vivo cardiac harm and toxicity of e-liquid flavorings, and 3)- To investigate the role of oxidative stress in mediating the cardiac toxicity of e-liquid flavorings. We believe that this proposal will produce new, useful and focused insights into the potential in-vivo and in-vitro adverse effects of e-liquid flavorings using functional and cellular indicators of cardiac harm. Our findings will hopefully be valuable in increasing our understanding of whether the flavoring aspect of ENDS products design could cause cardiac in- vivo and in- vitro toxicities.

电子尼古丁输送系统中香料的心脏毒性吸烟人数正在减少，但电子尼古丁输送系统 (ENDS) 的使用却在减少烟草的心脏毒性越来越受欢迎，特别是在青少年和年轻人中。吸烟已被广泛研究并得到充分证实，ENDS可能具有心脏毒性产品及其设计特征（例如添加的调味剂）在很大程度上尚未得到充分探索。电子尼古丁输送形式称为电子烟，使用“电子液体”来产生“电子蒸气”，这是一种可吸入的在这里，我们建议研究电子烟液如何产生含有尼古丁和香料的烟雾状雾化混合物。在细胞培养和动物模型中，不同的口味会影响心脏的体外和体内毒性。假设吸入电子烟液调味剂会增加心脏氧化应激，导致我们将针对三个目标进行研究：1)- 研究电子烟液调味剂的体外心脏危害和毒性，2)- 研究体内心脏危害电子烟液调味剂的危害和毒性，以及 3)- 研究氧化应激在介导我们相信该提案将产生新的、有用的和有针对性的。利用功能性和细胞性深入了解电子烟液调味剂潜在的体内和体外不良影响我们的研究结果有望有助于增加我们对心脏损伤的了解。 ENDS 产品设计的香料方面可能会导致心脏体内和体外毒性。