Mitogen activated protein kinases regulation-PTH/PTHrP

丝裂原激活蛋白激酶调节-PTH/PTHrP

• 批准号: 7541265
• 负责人: HESHAM A TAWFEEK
• 金额: $ 0.11万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7541265
• 关键词: Apoptosis; Basic Science; Binding; Biochemical; Biological; Cell Line; Cell Proliferation; Cells; Collagen; Cultured Cells; Cyclic AMP; Data; Exhibits; Family; Focal Adhesion Kinase 1; G-Protein-Coupled Receptors; Genetically Engineered Mouse; Goals; Homeostasis; Ions; Knock-in Mouse; Laboratories; MAPK11 gene; Mediating; Mitogen Activated Protein Kinase 1; Mitogen-Activated Protein Kinases; Molecular; Molecular Biology; Mus; Numbers; Osteoblasts; Parathyroid Hormone Receptor; Parathyroid Hormone Receptors; Parathyroid Hormones; Pathway interactions; Peptide antibodies; Phosphorylation; Process; Property; Receptor Protein-Tyrosine Kinases; Regulation; Role; Scientist; Signal Transduction; Testing; Training; Transgenic Mice; extracellular; hormone regulation; human PTH protein; in vivo; kidney cell; mouse model; parathyroid hormone-related protein; promoter; protein-tyrosine kinase c-src; receptor; response; src-Family Kinases; substantia spongiosa; tool; transgene expression

The ultimate goal of this K01 proposal is to provide the applicant with additional training in basic science and molecular biology to establish himself as an independent scientist. The project elucidates the molecular mechanisms and the function of PTH/PTHrP receptor regulation of Extracellular signal regulated (ERK) mitogen-activated protein (MAP) kinases. The role of non-receptor tyrosine kinases as well as the role of receptor phosphorylation in PTH activation of ERK1/2 MAP kinases in osteoblastic cell lines will be explored. Furthermore, the role of ERK1/2 MAP kinases in osteoblastic cell proliferation, differentiation and apoptosis will be investigated. Primary osteoblastic cell culture from wild type and genetically engineered mice as well as several other biochemical and pharmacological tools will be employed to achieve the goals of the study.

该 K01 提案的最终目标是为申请人提供基础科学和 分子生物学使自己成为一名独立科学家。该项目阐明了分子 PTH/PTHrP受体调节细胞外信号调节(ERK)的机制和功能 丝裂原激活蛋白（MAP）激酶。非受体酪氨酸激酶的作用以及 将探讨成骨细胞系中 PTH 激活 ERK1/2 MAP 激酶的受体磷酸化。 此外，ERK1/2 MAP 激酶在成骨细胞增殖、分化和凋亡中的作用 将被调查。来自野生型和基因工程小鼠的原代成骨细胞培养物 由于将采用其他几种生化和药理学工具来实现研究目标。