Antibacterial nanoparticles as insecticide synergists and insect growth regulators for improved control of cockroach infestations

抗菌纳米颗粒作为杀虫剂增效剂和昆虫生长调节剂，可改善对蟑螂侵扰的控制

基本信息

批准号：
10241828
负责人：
Jose Enrique Pietri
金额：
$ 25.76万
依托单位：
APEX BAIT TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-09 至 2023-03-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY The prevalence of asthma in the United States is approximately 8% (>25 million people). In African Americans, the prevalence is >11%. A majority of asthma sufferers are sensitized to allergens, including those shed in the feces, saliva, and exuviae of cockroaches. Thus, exposure to cockroach infestation is recognized as both a significant risk factor for the development of allergic asthma and a potent trigger of asthma attacks. The asthma risk from cockroach exposure is particularly high in inner-city residences where elevated levels of cockroach allergens are common. For example, some studies have shown that cockroach allergens are found at high concentrations associated with asthma in >45% of high-rise apartments in the U.S., and >60% of asthmatic children in U.S. inner cities are sensitized to cockroach allergens. Treating active cockroach infestations can significantly reduce allergen levels within structures and is one strategy to improve asthma symptoms that is recommended by the National Asthma Education and Prevention Board. The German cockroach, Blatella germanica, is the most widespread and problematic cockroach pest in urban environments. The use of insecticidal baits has traditionally been the most effective method for control of this species. However, the spread of insecticide resistance across populations of B. germanica can reduce the efficacy of insecticidal interventions, creating a need for improved tools. Our research team recently reported that the gut microbiota contributes to insecticide resistance in B. germanica. We also found that disrupting the gut microbiota decreases reproductive fitness. Accordingly, we predict that targeting the microbiota of German cockroaches by incorporating antibacterial metal oxide nanoparticles into insecticidal bait formulations could be an effective strategy to improve control of insecticide-resistant infestations in the field. We propose three specific aims to test the viability of this approach against cockroaches resistant to diverse insecticides using two types of metal oxide nanoparticles with known antibacterial properties (ZnO and CuO). In aim 1, we will determine if antibacterial nanoparticles can increase the efficacy of several commonly used bait insecticides against resistant cockroach strains. In aim 2, we will determine if antibacterial nanoparticles in bait can adversely affect cockroach life history. In aim 3, we will determine if nanoparticle-insecticide combination baits can eliminate resistant cockroach populations under semi-field conditions. It is expected that the results of our work will inform the development of novel insecticidal bait formulations to mitigate insecticide resistance and improve cockroach control, which has been demonstrated to reduce asthma morbidity and improve outcomes in human populations.

项目概要在美国，哮喘患病率约为 8%（>2500 万人）。在非裔美国人中，患病率>11%。大多数哮喘患者对过敏原敏感，包括那些在室内排出的过敏原。蟑螂的粪便、唾液和蜕皮。因此，接触蟑螂感染被认为是一种过敏性哮喘发生的重要危险因素和哮喘发作的有效触发因素。这在市中心住宅中，接触蟑螂导致的哮喘风险特别高，因为那里的蟑螂水平较高。蟑螂过敏原很常见。例如，一些研究表明，发现蟑螂过敏原在美国 > 45% 的高层公寓和 > 60% 的高层公寓中，其浓度与哮喘相关美国内城区的哮喘儿童对蟑螂过敏原敏感。治疗活跃的蟑螂感染可以显着降低建筑物内的过敏原水平，是改善哮喘的一种策略国家哮喘教育和预防委员会建议的症状。德国人蟑螂（Blatella germanica）是城市环境中分布最广泛、问题最严重的蟑螂害虫。传统上，使用杀虫诱饵是控制该物种的最有效方法。然而，杀虫剂抗药性在德国小蠹种群中的传播会降低杀虫剂的功效杀虫干预措施，需要改进的工具。我们的研究小组最近报告说，肠道微生物群有助于德国小蠹的杀虫剂抗性。我们还发现，破坏肠道微生物群降低生殖健康。因此，我们预测针对德国微生物群通过将抗菌金属氧化物纳米颗粒加入到杀虫诱饵配方中可以有效消灭蟑螂改善田间抗药性侵染控制的有效策略。我们提出三个具体目的是测试这种方法对抗对多种杀虫剂具有抗药性的蟑螂的可行性两种具有已知抗菌特性的金属氧化物纳米颗粒（ZnO 和 CuO）。在目标 1 中，我们将确定抗菌纳米颗粒是否可以提高几种常用诱饵杀虫剂的功效对抗耐药蟑螂菌株。在目标 2 中，我们将确定诱饵中的抗菌纳米粒子是否可以对蟑螂的生活史产生不利影响。在目标 3 中，我们将确定纳米颗粒-杀虫剂组合是否具有诱饵作用可在半田间条件下消灭耐药蟑螂种群。预计我们的结果这项工作将为新型杀虫诱饵制剂的开发提供信息，以减轻杀虫剂抗药性和改善蟑螂控制，已被证明可以降低哮喘发病率并改善结果在人群中。