Antibacterial nanoparticles as insecticide synergists and insect growth regulators for improved control of cockroach infestations

抗菌纳米颗粒作为杀虫剂增效剂和昆虫生长调节剂，可改善对蟑螂侵扰的控制

基本信息

批准号：
10241828
负责人：
Jose Enrique Pietri
金额：
$ 25.76万
依托单位：
APEX BAIT TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-04-09 至 2023-03-31
项目状态：
已结题

项目摘要

PROJECT SUMMARY The prevalence of asthma in the United States is approximately 8% (>25 million people). In African Americans, the prevalence is >11%. A majority of asthma sufferers are sensitized to allergens, including those shed in the feces, saliva, and exuviae of cockroaches. Thus, exposure to cockroach infestation is recognized as both a significant risk factor for the development of allergic asthma and a potent trigger of asthma attacks. The asthma risk from cockroach exposure is particularly high in inner-city residences where elevated levels of cockroach allergens are common. For example, some studies have shown that cockroach allergens are found at high concentrations associated with asthma in >45% of high-rise apartments in the U.S., and >60% of asthmatic children in U.S. inner cities are sensitized to cockroach allergens. Treating active cockroach infestations can significantly reduce allergen levels within structures and is one strategy to improve asthma symptoms that is recommended by the National Asthma Education and Prevention Board. The German cockroach, Blatella germanica, is the most widespread and problematic cockroach pest in urban environments. The use of insecticidal baits has traditionally been the most effective method for control of this species. However, the spread of insecticide resistance across populations of B. germanica can reduce the efficacy of insecticidal interventions, creating a need for improved tools. Our research team recently reported that the gut microbiota contributes to insecticide resistance in B. germanica. We also found that disrupting the gut microbiota decreases reproductive fitness. Accordingly, we predict that targeting the microbiota of German cockroaches by incorporating antibacterial metal oxide nanoparticles into insecticidal bait formulations could be an effective strategy to improve control of insecticide-resistant infestations in the field. We propose three specific aims to test the viability of this approach against cockroaches resistant to diverse insecticides using two types of metal oxide nanoparticles with known antibacterial properties (ZnO and CuO). In aim 1, we will determine if antibacterial nanoparticles can increase the efficacy of several commonly used bait insecticides against resistant cockroach strains. In aim 2, we will determine if antibacterial nanoparticles in bait can adversely affect cockroach life history. In aim 3, we will determine if nanoparticle-insecticide combination baits can eliminate resistant cockroach populations under semi-field conditions. It is expected that the results of our work will inform the development of novel insecticidal bait formulations to mitigate insecticide resistance and improve cockroach control, which has been demonstrated to reduce asthma morbidity and improve outcomes in human populations.

项目摘要美国哮喘的患病率约为8％（> 2500万人）。在非裔美国人中，患病率> 11％。大多数哮喘患者都对过敏原敏感，包括脱落的哮喘患者粪便，唾液和蟑螂。因此，暴露于蟑螂侵染被认为是过敏性哮喘发展和哮喘发作有效触发的重要危险因素。这蟑螂暴露的哮喘风险在城市内部住宅中尤其高。蟑螂过敏原是常见的。例如，一些研究表明发现蟑螂过敏原在美国> 45％的高层公寓中，高浓度与哮喘相关，> 60％美国内部城市中的哮喘儿童对蟑螂过敏原敏感。治疗活跃的蟑螂侵扰可以显着降低结构内的过敏原水平，是改善哮喘的一种策略国家哮喘教育和预防委员会建议的症状。德国人 Blatella germanica蟑螂是城市环境中最普遍，最有问题的蟑螂害虫。传统上，使用杀虫诱饵是控制该物种的最有效方法。但是，杀虫剂耐药性在甲状腺芽孢杆菌的种群中的传播可以降低杀虫剂干预措施，需要改进工具。我们的研究小组最近报告了肠道微生物群在B. dermanica中有助于杀虫剂的耐药性。我们还发现这破坏了肠道微生物群降低生殖适应性。因此，我们预测针对德国菌群通过将抗菌金属氧化金属纳米颗粒掺入杀虫剂制剂中，可以是蟑螂一种有效的策略，是改善对野外抗杀虫剂侵扰的控制。我们提出了三个特定的目的是测试这种方法对使用抗多种杀虫剂的蟑螂的生存能力具有已知抗菌特性（ZnO和CuO）的两种类型的金属氧化物纳米颗粒。在AIM 1中，我们将确定抗菌纳米颗粒是否可以提高几种常用诱饵杀虫剂的功效抵抗抗蟑螂菌株。在AIM 2中，我们将确定诱饵中的抗菌纳米颗粒是否可以不利影响蟑螂的生活历史。在AIM 3中，我们将确定纳米颗粒 - 杀菌剂的组合是否诱饵可以在半野外条件下消除抗蟑螂种群。预计我们的结果工作将为新型杀虫剂制剂的发展提供信息，以减轻杀虫剂的耐药性和改善蟑螂控制，已被证明可以降低哮喘发病率并改善结果在人类人群中。