Functional Organization of Auditory Corticofugal Circuits

听觉皮质回路的功能组织

• 批准号: 10239224
• 负责人: Ross Stewart Williamson
• 金额: $ 19.55万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-24 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10239224
• 关键词: Address; Affect; Amygdaloid structure; Anatomy; Area; Arousal; Attention; Auditory; Auditory area; Axon; Behavior; Biophysics; Biosensor; Brain; Brain region; Calcium; Caliber; Cerebral cortex; Cerebrum; Cognition; Corpus striatum structure; Data; Development; Dissection; Foundations; Future; Genetic; Goals; Individual; Inferior Colliculus; Label; Learning; Locomotion; Mediating; Microscope; Midbrain structure; Monitor; Morphology; Motor Activity; Mus; Neurons; Optics; Output; Pathway interactions; Pattern; Perception; Perceptual Disorders; Physiological; Physiology; Play; Population; Population Process; Process; Property; Pupil; Research Personnel; Rewards; Role; Route; Sensory; Series; Signal Transduction; System; Techniques; Thalamic structure; Transgenic Mice; Viral; Work; auditory nuclei; auditory pathway; auditory processing; auditory stimulus; cell type; cognitive function; experimental study; in vivo; insight; nervous system disorder; neural circuit; neuroregulation; novel; rabies viral tracing; response; selective expression; two-photon

Project Summary The ability to both route and coordinate the propagation of sensory information to downstream areas is a critical feature of cognitive function. The deep layers of the auditory cortex (ACtx) give rise to a massive projection system that exerts influence over multiple downstream brain areas. Sub-cerebral projection neurons (SPNs) within layer 5b, with their elaborate dendritic processes and far-ranging axons, have long been regarded as canonical “broadcast” neurons, pooling inputs from the upper cortical layers and transmitting signals to widespread downstream targets. Our recent work has shown that at least some SPNs that project to the midbrain also collateralize in the thalamus, striatum, and amygdala. Nevertheless, existing anatomical descriptions of this projection system are lacking, and the extent to which SPN populations broadcast information to several downstream targets simultaneously, as opposed to transmitting auditory signals to a single discrete region is not known. Despite their widespread influence, the neurons contributing to this corticofugal projection system have largely defied in vivo physiological characterization. Thus, the kind of sensory information that is routed to downstream targets remains to be established. Given that some SPN target regions reside within the limbic and reward systems of the brain, it is also important to assess how non- sensory factors related to internal state (such as arousal and locomotion) affect sensory processing in SPN populations that route information to distinct targets. Recent technical developments now make it possible to anatomically characterize the input/output circuitry of specific neuron classes in addition to being able to isolate these neurons in vivo to document their specific contributions to auditory processing. Here, we propose to use a battery of anatomical, optical, and viral approaches to elucidate the anatomical connectivity of SPN populations in mouse ACtx, to understand what information these SPN populations convey to downstream areas, and to describe how this information can be modulated by internal state. These data will provide a foundation for a subsequent R01 that will determine how different ACtx projections can influence perception and mediate behavior.

项目摘要 路由和协调感官信息传播到下游区域的能力是 认知功能的关键特征。听觉皮层的深层（actx）产生了巨大的 导出对多个下游大脑区域影响的投影系统。脑外投射神经元 （SPNS）在第5B层中，其精细的树突过程和远距离轴突长期以来一直是 被视为规范的“广播”神经元，从上层皮质层汇总输入并传输 信号到宽度下游目标。我们最近的工作表明，至少有一些该项目 中脑还在丘脑，纹状体和杏仁核中取代。然而，现有的解剖学 缺乏此投影系统的描述，以及SPN人群广播的程度 简单地向几个下游目标的信息信息，而不是将听觉信号传输到 单个离散区域尚不清楚。尽管影响宽度，但神经元为此做出了贡献 皮质型投射系统在体内生理表征上已在很大程度上被脱落。那是一种 路由到下游目标的感官信息尚待建立。考虑到一些SPN 目标区域位于大脑的边缘和奖励系统中，评估非 - 与内部状态有关的感觉因素（例如唤醒和运动）会影响SPN中的感觉处理 将信息路由到不同目标的人群。 现在，最近的技术发展使解剖学表征输入/输出电路成为可能 除了能够在体内分离这些神经元以记录其特定的特定神经元类别 对听觉处理的贡献。在这里，我们建议使用一系列解剖，光学和病毒 阐明小鼠ACTX中SPN种群的解剖连通性的方法，以了解什么 这些SPN种群传达给下游地区的信息，并描述该信息如何 由内部状态调节。这些数据将为后续R01提供基础，该基础将决定如何确定 不同的ACTX项目可以影响感知并介导行为。