Program Integration and Management

项目整合与管理

• 批准号: 10268361
• 负责人: MARIANNE BERWICK
• 金额: $ 1.2万
• 依托单位: UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10268361
• 关键词: Adjuvant Therapy; American Joint Committee on Cancer; Bioinformatics; Biological Assay; Biological Markers; Biology; Blood; Breslow Thickness; Characteristics; Collaborations; Communication; Communication Tools; DNA; Data; Diagnosis; Evaluation; Goals; Individual; Laboratories; Messenger RNA; Methylation; MicroRNAs; Monitor; Mutation; Mutation Analysis; Normal tissue morphology; Patients; RNA; Research; Research Personnel; Resource Sharing; Scanning; Sex Differences; Solar elastosis; Stage at Diagnosis; TNM; Teleconferences; Trust; Tumor-Infiltrating Lymphocytes; Work; analysis pipeline; data harmonization; data sharing; follow-up; lymph nodes; melanoma; member; methylome; nano-string; outcome forecast; programs; tool; tumor

The Program Integration and Management component of this Program Project will assure communication among the Projects and Cores so that each Project and Core is able to conduct their own research and to interface among the others. Furthermore, this component will assure the responsible management of the Projects and Cores of the Program Project. As noted, the 3 projects with a laboratory component will use DNA or RNA from the exact same tumors. The Administrative Core has been responsible for developing the collaborations in order to obtain tumors and will maintain the harmonized data (see Appendix x), with the critical information as to Stage at diagnosis, year of diagnosis and follow up with survival information (Dead of Melanoma, Dead of Other Causes, and Alive at Follow up); the Biospecimen Core will receive the tumors, evaluate them for histopathologic characteristics (see Biospecimen Core), such as Breslow thickness, solar elastosis, tumor infiltrating lymphocytes (TILs), etc., send the H&E to UNC for Aperio ScanScope scanning to share with all investigators, extract DNA and RNA from each tumor, as well as uninvolved normal tissue (in cases where we don’t have germline DNA from blood or lymph nodes), send DNA to Project 1 for targeted mutation analysis (both tumor and germline, including evaluation of CNVs), send DNA to Project 2 for methylation assays, and send RNA to Project 3 for analysis of microRNA and mRNA using Nanostring. The Bioinformatics Core will develop tools for storage, management and sharing data generated by individual projects. It will also assist each project in analysis beyond the “pipeline” analyses that will be available for the IMPACT assay and the methylation assays, as well as the miRNA and mRNA analyses. These data will be available to Project 4 as will the raw data from each project, so that the Integration can occur as described. Progress in each project will be monitored during the bi-weekly Teleconferences as the PI’s of each project (Berwick, Thomas, Hernando-Monge and Shen) and core (Berwick, Thomas, Busam and Gorlov) are on the Steering Committee along with several key members (Armos, Begg, Ernstoff and Orlow). We have established a strong trusting relationship among the individuals and difficult issues have already been worked out and so we anticipate continuation of these excellent interactions.

该计划项目的计划集成和管理部分将确保沟通 项目和核心之间的联系，以便每个项目和核心都能够进行自己的研究并 此外，该组件将确保负责任的管理。 项目和项目核心 如前所述，具有实验室部分的 3 个项目将使用 DNA。 或来自完全相同肿瘤的RNA 管理核心负责开发该技术。 合作以获得肿瘤并将维护统一的数据（见附录x）， 关键信息包括诊断阶段、诊断年份和随访生存信息（死亡日期） 黑色素瘤、因其他原因死亡和随访时存活）；生物样本核心将接收肿瘤， 评估它们的组织病理学特征（参见生物样本核心），例如 Breslow 厚度、太阳光 弹性组织变性、肿瘤浸润淋巴细胞 (TIL) 等，将 H&E 发送至 UNC 进行 Aperio ScanScope 扫描 与所有研究人员分享，从每个肿瘤以及未受累的正常组织（在 如果我们没有来自血液或淋巴结的种系 DNA），请将 DNA 发送至项目 1 以进行靶向 突变分析（肿瘤和种系，包括 CNV 评估），将 DNA 发送至项目 2 进行 甲基化检测，并将 RNA 发送至 Project 3，使用 Nanostring 分析 microRNA 和 mRNA。 生物信息学核心将开发用于存储、管理和共享个人产生的数据的工具 它还将协助每个项目进行超出可用于“管道”分析的分析。 IMPACT 测定和甲基化测定以及 miRNA 和 mRNA 分析将是这些数据。 项目 4 以及每个项目的原始数据都可用，以便可以按照描述进行集成。 每个项目的进展情况将在每两周一次的电话会议中作为每个项目的 PI 进行监控 （Berwick、Thomas、Hernando-Monge 和 Shen）和核心（Berwick、Thomas、Busam 和 Gorlov）均在 我们成立了指导委员会以及几位主要成员（Armos、Begg、Ernstoff 和 Orlow）。 个人之间建立了牢固的信任关系，困难的问题已经解决，因此 我们预计这些出色的互动将继续下去。