Multifunctional Nanoplatforms-Based Capture and identification of Circulating Tumor Biomarkers for Early Diagnosis of Lung Cancer

基于多功能纳米平台的循环肿瘤生物标志物的捕获和鉴定用于肺癌的早期诊断

• 批准号: 10256728
• 负责人: Paresh Chandra Ray
• 金额: $ 18.87万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-08 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10256728
• 关键词: Affect; African American; American Cancer Society; Antibodies; Bacteria; Biological Assay; Blood; Blood Screening; Blood specimen; CD81 gene; CTAG1 gene; Cancer Detection; Cancer Etiology; Cancer Patient; Cancer cell line; Carbon; Cells; Centers for Disease Control and Prevention (U.S.); Cessation of life; Clinical; Detection; Development; Devices; Diagnosis; Diagnostic; Disease; Early Diagnosis; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor Receptor; Epithelial; Ethnic group; Flow Cytometry; Fluorescence; Genes; Goals; Image; Imaging Techniques; Incidence; Magnetism; Malignant Neoplasms; Malignant neoplasm of lung; Mediator of activation protein; Medical center; Mesenchymal; Methods; Mississippi; National Cancer Institute; Neoplasm Circulating Cells; Neoplasm Metastasis; Outcome; Pathologist; Patients; Pharmaceutical Preparations; Population; Population Heterogeneity; Process; Proteins; Race; Reporting; Research; Testing; Time; Tumor Markers; Tumor-Derived; United States; Universities; Viola; Vision; Whole Blood; Work; base; blood-based biomarker; cancer biomarkers; cancer cell; cancer diagnosis; circulating biomarkers; clinical application; clinically relevant; design; detection assay; disease diagnosis; ethnic diversity; exosome; experimental study; graphene; health disparity; healthy volunteer; improved; lung cancer cell; mortality; nanomagnetic; nanoparticle; nanotechnology platform; novel; peripheral blood; racial diversity

Significant disparities in incidence and mortality rates for lung cancer are reported among ethnically and racially diverse population. African Americans suffer from lung cancer more than any other population. High mortality rate in lung cancer is due to being diagnosed at a later stage, where treatment options are limited. Our long-term goal is to lay the groundwork for developing novel nanoplatform based device which will provide a more accurate determination of the circulating cancer biomarkers from lung cancer patients in blood screening facilities. The objective of this proposal is to design magnetic-fluorescence multifunctional nanoplatform based device to capture, isolate and detect circulating tumor cells (CTCs) and tumor derived exosomes from blood samples in lung cancer patients and to establish correlations of these findings with survival. Our central hypothesis is that bio-conjugated multicolor fluorescence carbon dots attached magnetic nano-platform can be used to capture multiple types of CTCs and circulating exosomes from blood samples, and as a facile multicolor fluorescence probe for accurate identification of circulating biomarkers. The central hypothesis will be tested by pursuing two specific aims: 1) Developing fluorescence- magnetic nanoplatform for capture, and identification of multiple types of CTCs from lung cancer patient blood and 2) Capture and identification of tumor derived exosomes using nanoplatform and demonstration of clinical applications. The proposed research is significant, because with high sensitivity and real-time detection possibility, CDs attached magnetic nano-platform will be highly promising for detection and identification of several subpopulation types of CTCs and exosomes. The proximate expected outcome of this work is improving the capability of identifying multiple clinically relevant blood based biomarkers in blood screening facilities.

据报道，肺癌的发病率和死亡率差异很大 在种族和种族多样化的人群中。非裔美国人患有肺部 癌症比任何其他人群都要多。肺癌的高死亡率是由于 在以后的阶段被诊断为有限的治疗选择。我们的长期 目标是为开发基于纳米板的新型设备奠定基础 将提供更准确的确定循环的癌症生物标志物 血液筛查设施中的肺癌患者。该提议的目的是 设计磁性荧光多功能基于纳米板的设备，以捕获 分离和检测循环的肿瘤细胞（CTC）和从肿瘤衍生的外泌体。 肺癌患者的血液样本并建立这些发现的相关性 生存。我们的中心假设是生物偶联的多色荧光 碳点附着磁性纳米平台可用于捕获多种类型 来自血液样本的CTC和循环外泌体的循环，作为一个简便的多色 荧光探针以准确鉴定循环生物标志物。中央 假设将通过追求两个具体目的来检验：1）发展荧光 - 用于捕获的磁性纳米植物，并从中识别多种类型的CTC 肺癌患者血液和2）捕获和鉴定造成的肿瘤 使用纳米植物和临床应用的表现。这 拟议的研究很重要，因为具有较高的灵敏度和实时 检测可能性，CD附着的磁性纳米平台将是高度有希望的 用于检测和鉴定CTC的几种亚群类型和 外泌体。这项工作的直接预期结果正在提高能力 在血液筛查中识别多种临床相关的基于血液的生物标志物 设施。