RAMAN AND HYPER RAYLEIGH SPECTROSCOPY OF NANOSCALE BIOLOGICAL INTERFACE

纳米级生物界面的拉曼和超瑞利光谱

• 批准号: 7336111
• 负责人: Paresh Chandra Ray
• 金额: $ 1.58万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336111
• 关键词: RAMAN; HYPER; RAYLEIGH; SPECTROSCOPY; NANOSCALE; RAMAN; HYPER; RAYLEIGH; SPECTROSCOPY; NANOSCALE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We have demonstrated for the first time that the hyper¿Rayleigh Scattering (HRS), which has emerged over the past decade as a powerful method to determine the microscopic non-linear optical (NLO) properties of species in solution, could achieve the ultra-sensitive detection of single base-pair mismatch in oligonucleotide strands without any covalent tagging of dyes. We have also reported 2 laser induced fluorescence (LIF) studies of ss-DNA hybridization based on fluorescence quenching by gold nanoparticles through a distant dependent process. We have shown that the fluorescence of ss-DNA increases by a factor of 100 when it binds to a complementary DNA while the addition of single-base mismatch DNA had no effect on the fluorescence efficiency.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。我们首次证明，在过去十年中，超级瑞利散射（HRS（HRS）已成为一种有力的方法来确定溶液中物种的微观非线性光学（NLO）特性，可以实现对寡核苷酸链链的单碱基对不合格的超敏感性检测，而没有任何covalents colvalents colvalents covalents not colvalents。我们还报道了基于金纳米颗粒通过远处依赖的过程，基于金纳米颗粒荧光淬灭SS-DNA杂交的2个激光诱导的荧光（LIF）研究。我们已经表明，当SS-DNA的荧光与完整的DNA结合时，SS-DNA的荧光增加了100倍，而单基不匹配DNA的添加对荧光效率没有影响。