Synthetic Chemical Biology

合成化学生物学

基本信息

批准号：
10245047
负责人：
BLAKE PETERSON
金额：
$ 16.16万
依托单位：
UNIVERSITY OF KANSAS LAWRENCE
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-07-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10245047
关键词：
Address Animal Model Anti-Infective Agents Biochemistry Biological Biological Assay Biology Biomedical Research Biotin Cell Culture Techniques Cell model Cells Cellular Assay Centers of Research Excellence Chemicals Chemistry Client Communicable Diseases Communities Core Facility Custom DNA Sequencing Facility Disease Pathway Equipment Evaluation Faculty Foundations Funding Generations Genes Goals Grant Human Resources Imaging Device In Vitro Infrastructure Kansas Label Lead Methods Microfabrication Microfluidics Microscopy Mission Modeling Molecular Molecular Analysis Molecular Probes National Institute of General Medical Sciences Organic Chemistry Peptides Pharmaceutical Chemistry Phase Property Research Personnel Resolution Resources Rodent Services Structure Tracer Universities Work Zebrafish biological systems cellular imaging chemical synthesis design drug discovery experience fluorescence imaging fluorophore genome sequencing high resolution imaging high throughput screening improved in vivo novel operation outreach peptidomimetics programs small molecule success

项目摘要

PROJECT SUMMARY The Synthetic Chemical Biology Core (SCBC) was established in July 2016 from the merger of the Center for Molecular Analysis of Disease Pathways (CMADP) Molecular Probes Core (MPC) with the newly established Chemical Biology of Infectious Disease (CBID) Medicinal Chemistry Core (MCC). The synthetic capabilities of the SCBC will fulfill the specific aims of both COBRE centers. The mission for the SCBC in Phase II is to provide the junior faculty affiliated with CMADP, as well as other investigators in the state of Kansas, with novel molecular probes of biological systems. The SCBC will also offer the capability to evaluate these probes in vitro in whole- cell bioassays, and in vivo in zebrafish, an increasingly important model organism for biomedical research. The SCBC will also fulfill CBID’s missions concerning anti-infective drug discovery. These projects will be funded through the CBID. The SCBC Director is responsible for coordinating all SCBC Core lab activities to both COBRE centers. The SCBC will offer expert design of molecular probes and synthesis of both small molecules and peptides, with an emphasis on the generation of fluorescent and other tagged molecules. SCBC will also offer bioassays of molecular probes, including whole cell assays and assays using zebrafish, to bridge a gap that currently exists between very high resolution imaging of cells in culture and low resolution imaging of fluorescent tracers in rodents. The newly established SCBC would function synergistically with other CMADP-funded Core facilities, including the Genome Sequencing Core, to identify genes that impact functions of probes in vivo, and the Microfabrication and Microfluidics Core, to develop devices for imaging and chemical analysis. This integration is designed to empower faculty to use chemical biology approaches to investigate biological problems. The SCBC provides investigators with access to highly qualified personnel with experience in synthetic organic chemistry, biochemistry, microscopy, and approaches for optimization of biological properties of synthetic molecules. The Center has two Co-Core Leaders from the Department of Medicinal Chemistry at KU. Prof. Blake Peterson will continue to be the Leader of the CMADP’s part of the SCBC. Prof. Tom Prisinzano is the Leader of the CBID part of the SCBC. The Director of SCBC is Dr. Chamani Perera. The specific aims that will be addressed by the SCBC for this Phase II proposal are: (1) to continue to integrate resources from the proposed COBRE Center for Molecular Analysis of Disease Pathways Phase II with the existing COBRE Center for Chemical Biology of Infectious Disease Phase I to further establish the new SCBC, gain economies of scale, and serve faculty affiliated with the COBRE CMADP; (2) design and synthesize molecular probes of biological systems for faculty investigators; (3) enable faculty clients to conduct bioassays in cell culture and zebrafish models; and (4) provide outreach and administrative support necessary to manage Core activities, and offer services to the larger biomedical research community in the state of Kansas.

项目概要合成化学生物学核心（SCBC）成立于2016年7月，由合成化学生物学中心合并而成。疾病途径分子分析（CMADP）分子探针核心（MPC）与新建立的传染病化学生物学 (CBID) 药物化学核心 (MCC) 的合成能力。 SCBC 将实现两个 COBRE 中心的具体目标 SCBC 在第二阶段的使命是提供服务。 CMADP 附属的初级教员以及堪萨斯州的其他研究人员，利用新颖的分子 SCBC 还将提供在体外对这些探针进行整体评估的能力。细胞生物测定，以及斑马鱼的体内实验，斑马鱼是生物医学研究中日益重要的模式生物。 SCBC 还将履行 CBID 有关抗感染药物发现的使命。这些项目将获得资助。通过 CBID，SCBC 总监负责协调 COBRE 的所有 SCBC 核心实验室活动。 SCBC 将提供分子探针的专业设计以及小分子和小分子的合成。 SCBC 还将提供肽，重点是荧光和其他标记分子的生成。分子探针的生物测定，包括全细胞测定和使用斑马鱼的测定，以弥补差距目前存在于培养细胞的极高分辨率成像和荧光的低分辨率成像之间新成立的 SCBC 将与 CMADP 资助的其他核心发挥协同作用。设施，包括基因组测序核心，用于识别影响体内探针功能的基因，以及微加工和微流体核心，开发用于成像和化学分析的设备。整合旨在使教师能够使用化学生物学方法来研究生物学问题。 SCBC 为调查人员提供了接触具有合成有机经验的高素质人员的机会化学、生物化学、显微镜和合成材料生物特性的优化方法该中心有两名来自 KU 药物化学系的 Blake 教授。 Peterson 将继续担任 SCBC 的 CMADP 部分的领导者，Tom Prisinzano 教授担任领导者。 SCBC 的 CBID 部分的主任是 Chamani Perera 博士。 SCBC 针对第二阶段提案提出的建议是： (1) 继续整合提案中的资源 COBRE 疾病途径分子分析中心 II 期与现有的 COBRE 疾病途径分子分析中心传染病化学生物学一期进一步建立新的SCBC，获得规模经济，并为COBRE CMADP附属教师服务；（2）设计和合成生物分子探针； (3) 使教师客户能够进行细胞培养和斑马鱼的生物测定模型；(4) 提供管理核心活动所需的外展和行政支持，并提供为堪萨斯州更大的生物医学研究界提供服务。