Antidiabetic Constituents from the Dominican Medicinal Plant Momordica charantia

多米尼加药用植物苦瓜的抗糖尿病成分

• 批准号: 7575803
• 负责人: Amy Celeste Keller
• 金额: $ 2.67万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7575803
• 关键词: Affect; Antidiabetic Drugs; Biological Assay; Blood Glucose; Cell Line; Cells; Chemical Structure; Chronic Disease; Collection; Communities; Complementary and alternative medicine; Cucurbitaceae; Diabetes Mellitus; Diagnosis; Disease; Dominican; Fruit; Goals; Herbal Medicine; Hispanics; Human; Hyperglycemia; Hypoglycemia; Hypoglycemic Agents; In Vitro; Insulin; Insulin-Dependent Diabetes Mellitus; Investigation; Islet Cell; Juice; Left; Measures; Medical; Medicinal Plants; Medicine; Metformin; Methods; Modeling; Momordica charantia; Morbidity - disease rate; Non-Insulin-Dependent Diabetes Mellitus; Not Hispanic or Latino; Obesity; Oral; Pharmaceutical Preparations; Phytochemical; Pioglitazone; Plants; Plasma; Population; Rattus; Research; Research Technics; Rodent Model; Techniques; Testing; Training; United States; Whole Blood; base; career; cell growth; diabetic; follow-up; improved; in vitro Assay; in vitro activity; in vitro testing; in vivo; insulin secretion; mortality; mouse model; skills; stem

DESCRIPTION (provided by applicant): Momordica charantia is a widely-used plant in traditional Dominican medicine for diabetes treatment. This project hypothesizes that the active compounds isolated from M. charantia fruits lower blood glucose concentrations via an increase in (3-cell replication and function, resulting in improved insulin secretion. To test this hypothesis, the fruits of M. charantia will be extracted, fractionated, and tested with a p-cell assay in order to identify the active fractions. Various chromatographic methods will be used to isolate the bioactive compounds from the active fractions. After isolation, the active compounds will be identified using modern phytochemical techniques and further tested in vitro using (3-cells to quantify the effects against an established oral hypoglycemic drug as a reference. Two of the bioactive compounds will be selected for in vivo testing based on their activity in the (3-cell line, uniqueness of chemical structure, and yield. The selected compounds will then be tested in a mouse model of obesity-induced hyperglycemia along with positive controls of commonly prescribed medications for Type II diabetes mellitus to functionally investigate the compounds' hypoglycemic activity. In addition, the active compounds isolated from the fruit will be quantified in the stems and leaves of M. charantia, as these parts of the plants are used in traditional Dominican medicine. Many traditional remedies have not been rigorously researched in the scientific and medical communities. Therefore, this project will provide training both in phytochemical techniques and research, and also in the skills necessary for the scientific research of herbal medicine. This interdisciplinary training is vital to a goal of a lifetime career in the rigorous scientific study of complementary and alternative medicine. Momordica charantia is a widely-used plant in treating one of the world's most threatening diseases, diabetes. Although this plant's antidiabetic activity has been researched, very few studies have rigorously tested the bioactive M. charantia compounds for specific mechanisms of action. The results of this study will allow people to make more informed decisions about the use of M. charantia in diabetes treatment.

描述（由申请人提供）：苦瓜是多米尼加传统医学中广泛用于治疗糖尿病的植物。该项目假设从苦瓜果实中分离出的活性化合物通过增加 3 细胞复制和功能来降低血糖浓度，从而改善胰岛素分泌。为了验证这一假设，将提取苦瓜果实，分离，并通过 p 细胞测定进行测试，以鉴定活性成分，将使用各种色谱方法从活性成分中分离出生物活性化合物。分离后，将使用现代植物化学技术鉴定活性化合物并进一步测试。体外使用（3-细胞）来量化针对已确定的口服降血糖药物的效果作为参考。将根据其在（3-细胞系中的活性、化学结构的独特性、然后，将在肥胖引起的高血糖小鼠模型以及 II 型糖尿病常用处方药物的阳性对照中测试所选化合物，以从功能上研究这些化合物的降血糖活性。此外，从果实中分离出的活性化合物将在苦瓜的茎和叶中进行定量，因为植物的这些部分用于传统的多米尼加药物。许多传统疗法尚未经过科学和医学界的严格研究。因此，该项目将提供植物化学技术和研究以及草药科学研究所需技能的培训。这种跨学科培训对于实现补充和替代医学严格科学研究的终身职业目标至关重要。苦瓜是一种广泛用于治疗世界上最具威胁的疾病之一糖尿病的植物。尽管已经研究了这种植物的抗糖尿病活性，但很少有研究严格测试生物活性苦瓜化合物的具体作用机制。这项研究的结果将使人们能够就苦瓜在糖尿病治疗中的使用做出更明智的决定。