BLOOD-DERIVED DENDRITIC CELLS AND EPITHELIAL CELLS

血源性树突状细胞和上皮细胞

• 批准号: 7718409
• 负责人: JOAN REIBMAN
• 金额: $ 0.07万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7718409
• 关键词: Air; Allogenic; Blood; Blood Cells; Bronchoscopy; Cell Differentiation process; Cells; Cellular biology; Coculture Techniques; Computer Retrieval of Information on Scientific Projects Database; Dendritic Cells; Epithelial Cells; Funding; Grant; Immune response; Institution; Laboratories; Ligands; Monitor; Particulate Matter; Research; Research Personnel; Resources; Source; Stimulus; System; United States National Institutes of Health; cytokine; innovation; monocyte; precursor cell; respiratory

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study investigates the cell biology of dendritic cells (DC) in co-culture with airway epithelial cells. The objective is to determine if different DC subtypes predict the immune response (Th1 vs. Th2) to ambient particulate matter. Primary cells derived from blood and bronchoscopy will be cultured ex vivo. This highly innovative study should provide important basic information. The investigators will set up a new laboratory system in which allogeneic a) blood monocytes or b) blood CD34-positive cord-blood cells are cultured in a two-chamber apparatus that permits these cells to contact differentiated respiratory epithelial cells in the presence of unspecified mixtures of different cytokines, FIT3 ligand, ambient air particulate matter, etc. The blood monocytes or CD34-positive cells will be monitored by unspecified means to determine the various possible ways that the differentiation of these cells might be influenced by the epithelial cells and the stimuli to which the cell mixtures are exposed. The issue of the influence of epithelial cells on the differentiation of precursor cells into immature and mature DC is important and worthy of study.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究研究了与气道上皮细胞共培养中树突状细胞（DC）的细胞生物学。 目的是确定不同的DC亚型是否预测了对环境颗粒物质的免疫反应（Th1 vs.TH2）。 源自血液和支气管镜检查的原代细胞将被体内培养。 这项高度创新的研究应提供重要的基本信息。 研究人员将建立一个新的实验室系统，在该系统中同种异体a）血液单核细胞或b）血液CD34阳性脐带血细胞在两室的两个室设备中培养，该式植物使这些细胞与不同的呼吸道上皮细胞接触分化的呼吸上皮细胞，在存在不同的细胞的情况下，拟合33 ligient the ligiganters Mornity Morniques Mornity Mornity Mornity Mornity Mornity Morniques Mornity Mornity Mornity Monigant the-prols norder Mornity Morniques Mornity Mornity Mornicty Mornity Mornity Mornipers等。通过未指定的手段监测，以确定这些细胞的分化可能受上皮细胞以及暴露于细胞混合物的刺激的各种可能方式。 上皮细胞对前体细胞分化为不成熟和成熟DC的影响的问题很重要，值得研究。