Genome Sequencer FLX

基因组测序仪 FLX

基本信息

批准号：
7841366
负责人：
JOHN J. NGAI
金额：
$ 62.29万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-15 至 2011-04-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The 454/Roche GS-FLX pyrosequencer will be used for high-throughput DNA sequencing in applications demanding long reads (>400 nt) at high accuracy. The technology and characteristics of this sequencing technology is markedly different than for sequencers such as the Solexa/Illumina Genome Sequencer, which provide short (<75 nt) read lengths with a higher error rate. The ability to obtain long read lengths with high accuracy affords several critical advantages for a number of applications, including (1) the sequencing of novel genomes, (2) EST and transcriptome characterization in organisms lacking a sequenced or well-mapped genome, and (3) the analysis of alternative pre-mRNA splicing. The 454/Roche long-read sequencing technology will be applied to a diverse array of NIH-funded projects as well as other projects of high relevance to biomedical science, including the characterization of multigene families involved in sensory perception; the elucidation of the patterns and mechanisms of alternative pre-mRNA splicing; the characterization of genomes of genetic and evolutionary models of human development and disease; an analysis of the genetic loci associated with cancer; and an investigation of the susceptibility of mammalian cells to nutritional ions and environmental toxins. There are currently no 454 DNA sequencers on the UC Berkeley campus; acquisition of this instrumentation by the FGL will support researchers pursuing these projects, which promise to illuminate the fundamental mechanisms underlying human biology and disease.

描述（由申请人提供）：454/Roche GS-FLX 焦磷酸测序仪将用于需要高精度长读数（>400 nt）的应用中的高通量 DNA 测序。这种测序技术的技术和特点与 Solexa/Illumina 基因组测序仪等测序仪明显不同，后者提供短（<75 nt）读长，但错误率较高。获得高精度长读长的能力为许多应用提供了几个关键优势，包括（1）新基因组的测序，（2）缺乏测序或良好图谱基因组的生物体中的 EST 和转录组表征，以及（ 3)选择性前mRNA剪接的分析。 454/罗氏长读长测序技术将应用于 NIH 资助的各种项目以及与生物医学科学高度相关的其他项目，包括与感官知觉有关的多基因家族的表征；阐明选择性前 mRNA 剪接的模式和机制；人类发育和疾病的遗传和进化模型的基因组特征；与癌症相关的遗传位点分析；以及哺乳动物细胞对营养离子和环境毒素的敏感性的研究。目前加州大学伯克利分校校园内没有 454 台 DNA 测序仪； FGL 收购该仪器将支持研究人员开展这些项目，这些项目有望阐明人类生物学和疾病的基本机制。

项目成果

期刊论文数量（150）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The genetic basis of phage susceptibility, cross-resistance and host-range in Salmonella.

DOI：
10.1099/mic.0.001126
发表时间：
2021-12
期刊：
Microbiology (Reading, England)
影响因子：
0
作者：
Adler BA;Kazakov AE;Zhong C;Liu H;Kutter E;Lui LM;Nielsen TN;Carion H;Deutschbauer AM;Mutalik VK;Arkin AP
通讯作者：
Arkin AP

Selective stalling of human translation through small-molecule engagement of the ribosome nascent chain.

DOI：
10.1371/journal.pbio.2001882
发表时间：
2017-03
期刊：
PLoS biology
影响因子：
9.8
作者：
Lintner NG;McClure KF;Petersen D;Londregan AT;Piotrowski DW;Wei L;Xiao J;Bolt M;Loria PM;Maguire B;Geoghegan KF;Huang A;Rolph T;Liras S;Doudna JA;Dullea RG;Cate JH
通讯作者：
Cate JH

Nanoparticle delivery of Cas9 ribonucleoprotein and donor DNA in vivo induces homology-directed DNA repair.

DOI：
10.1038/s41551-017-0137-2
发表时间：
2017
期刊：
Nature biomedical engineering
影响因子：
28.1
作者：
Lee K;Conboy M;Park HM;Jiang F;Kim HJ;Dewitt MA;Mackley VA;Chang K;Rao A;Skinner C;Shobha T;Mehdipour M;Liu H;Huang WC;Lan F;Bray NL;Li S;Corn JE;Kataoka K;Doudna JA;Conboy I;Murthy N
通讯作者：
Murthy N

Synthetic and Evolutionary Construction of a Chlorate-Reducing Shewanella oneidensis MR-1.