Fluorescence tools that illuminate biology and inspire translation

阐明生物学并激发翻译的荧光工具

• 批准号: 10091496
• 负责人: Alanna Schepartz
• 金额: $ 68.78万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10091496
• 关键词: Area; Binding; Biology; Cells; Chemistry; Complex; Development; Disease; Elements; Eye; Fluorescence; Fluorescence Spectroscopy; Functional disorder; Future; Goals; Influentials; Knowledge; Laboratories; Molecular Analysis; Molecular Structure; Organelles; Property; Proteins; Research; Translations; Work; design; innovation; multidisciplinary; novel; programs; tool; tool development

Project Summary: Understanding the mechanisms of protein and organelle function and dysfunction in disease requires a detailed and dynamic analysis of molecular structures and interactions in live cells. The long-term goal of my laboratory is to develop tools that reveal these functional elements and apply them to discover significant biology and/or design molecules with useful properties. I am drawn to difficult, complex, multi-disciplinary problems where chemistry can catalyze exciting and non-incremental progress. We strive not simply to develop tools, but also to apply them in innovative ways to acquire new and influential knowledge. This rubric leads us naturally into diverse and emerging scientific areas. Fluorescence spectroscopy features prominently in the three research programs described herein. In two, a fluorescent tool we developed (section A) or innovated (section C) has already identified influential new biology; here future work capitalizes on this knowledge with an eye to translation. In the third, tool development is on- going (section B) but progress-to-date provides a clear roadmap for very exciting future applications.

项目摘要： 了解蛋白质和细胞器功能的机制以及疾病功能障碍 需要对活细胞中分子结构和相互作用进行详细的动态分析。 我实验室的长期目标是开发揭示这些功能元素的工具 并应用它们以发现具有有用特性的重要生物学和/或设计分子。我 被化学能力催化的困难，复杂，多学科问题所吸引 令人兴奋的和非进步的进展。我们不仅努力开发工具，还要应用 它们以创新的方式获取新的和有影响力的知识。这个标题自然带领我们 进入多样化和新兴的科学领域。荧光光谱特征在 本文描述的三个研究计划。在两个中，我们开发了一个荧光工具（部分 a）或创新（C节）已经确定了有影响力的新生物学；在这里，未来的工作 利用这些知识，以注重翻译。在第三章中，工具开发正在 - 前进（B节），但发展到日期为令人兴奋的未来提供了明确的路线图 申请。