A Comprehensive Functional Map of Human Protein-RNA Interactions

人类蛋白质-RNA 相互作用的综合功能图谱

基本信息

批准号：
10087952
负责人：
Brenton R. Graveley
金额：
$ 75.66万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-02-05 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10087952
关键词：
Antibodies Binding Binding Sites Biological Assay Biology Cell Line Cells Collection Complement Data Elements Encyclopedias Epitopes Foundations Genes Goals Human Human Biology Human Cell Line Human Genome Immunofluorescence Immunologic In Vitro Knock-out Maps Measurement Methods Pattern Post-Transcriptional Regulation Production Proteins RNA RNA Binding RNA Sequences RNA-Binding Proteins RNA-Protein Interaction Resources Role Specificity Untranslated RNA base human disease preference transcriptome transcriptome sequencing

Antibodies Binding Binding Sites Biological Assay Biology Cell Line Cells Collection Complement Data Elements Encyclopedias Epitopes Foundations Genes Goals Human Human Biology Human Cell Line Human Genome Immunofluorescence Immunologic In Vitro Knock-out Maps Measurement Methods Pattern Post-Transcriptional Regulation Production Proteins RNA RNA Binding RNA Sequences RNA-Binding Proteins RNA-Protein Interaction Resources Role Specificity Untranslated RNA base human disease preference transcriptome transcriptome sequencing

展开

项目摘要

PRODUCTION CORE – PROJECT SUMMARY The objective of the ENCORE (Encyclopedia of RNA Elements) project is to develop a foundational, functional map of protein-RNA interactions of RNA binding proteins (RBPs) encoded in the human genome, and the RNA elements they bind to across the transcriptome. These RNA elements, when expressed, form the basis of co- and post- transcriptional regulation of human genes. Our strategy consists of developing and integrating a physical map of 300 RBPs in two different human cell lines with transcriptome-wide measurements of the effects of depleting these RBPs, their localization patterns and their binding preferences independent of co-factor associations. In this data production core, we will capitalize on the highly efficient data production pipeline we have established over the past four years to generate RNA bind- n-seq, RNA-seq, protein localization and eCLIP data for an additional 300 RBPs. When combined with the data we generated over the past four years, these efforts will culminate in a comprehensive map of the functional RNA elements recognized by essentially all RBPs expressed in two human cell lines, representing approximately half of the known complement of human RBPs. In summary, the data we will produce in this project will enable a more systematic and comprehensive understanding of the role of RBPs and RNA biology in the contribution to human biology and disease.

生产核心 - 项目摘要 Encore的目的（RNA元素百科全书）项目是开发一个 RNA结合蛋白的蛋白RNA相互作用的基础，功能图（RBP）编码在人类基因组中，以及它们与跨整个的RNA元素转录组。这些RNA元素在表达时构成了共同和后的基础人类基因的转录调节。我们的策略包括开发和在两个不同的人类细胞系中将300 RBP的物理图与耗尽这些RBP的影响的全转录组的测量值本地化模式及其结合偏好独立于共同因素协会。在此数据生产核心中，我们将利用高效的数据我们在过去四年中建立的生产管道，以生成RNA结合 - N-Seq，RNA-Seq，蛋白质定位和Eclip数据的另外300 Rbps。什么时候结合我们在过去四年中产生的数据，这些努力将在识别的功能性RNA元件的综合图中达到高潮本质上，所有rbps均以两个人类细胞系表示，约为一半已知的人Rbps完成。总而言之，我们将在此中产生的数据项目将使人们能够更加系统地了解 RBP和RNA生物学对人类生物学和疾病的贡献。