Elucidating causes of vaginal symptoms using a multi-omics approach - Admin. Supplement

使用多组学方法阐明阴道症状的原因 - 管理员。

基本信息

批准号：
10091585
负责人：
LARRY J FORNEY
金额：
$ 9.99万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-05-13 至 2021-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10091585
关键词：
16S ribosomal RNA sequencing Address Adult Affect Amenorrhea Anaerobic Bacteria Antibiotics Atrophic Behavior Birth Canada Caring Cell Proliferation Characteristics Clinical Management Data Development Dilator Enrollment Epithelial Epithelium Exposure to Female Functional disorder Gender Gender Identity Genital system Glycogen Goals HIV Health Health protection Histologic Home environment Hormones Hygiene Immune Immunologic Factors Immunologics Individual Infection Inflammation Inflammation Mediators Intervention Link Logistic Regressions Lubricants Masculine Medical Medical Sociology Menopause Microbe Microbiology Modeling Operative Surgical Procedures Outcome Ovariectomy Pathology Patients Persons Pharmaceutical Preparations Physiology Population Production Quality of life Reporting Research Resolution Risk Role Sampling Scrotum Sex Behavior Sexual Health Sexually Transmitted Diseases Signs and Symptoms Skin Structure Surveys Symptoms Techniques Testing Testosterone United States United States National Institutes of Health Ursidae Family Vagina Woman base cis-female cisgender clinical care clinical development cohort cytokine gender nonconforming genital surgery hormone therapy immunological status improved innovation irritation male men microbial microbiome microbiome analysis microbiota multiple omics novel penile microbiota penis psychologic recruit research and development screening sex transgender transgender men transgender women vaginal microbiome vaginal microbiota vaginectomy

项目摘要

SUMMARY Transgender (trans) individuals are those who have a gender identity that does not match their birth-assigned sex. About 1.4 million adults in the United States identified as transgender in 2016. Trans people may take gender-affirming hormones or have undergone gender-affirming genital surgery. Little is known about approaches to sexual health screening and management, as well as risk for acquisition of sexually transmitted infections or development of other genital pathologies, in this population. The NIH has thus identified a major need for innovative research addressing the medical, sociological, psychological and structural causes and consequences of transgender and gender nonconforming identities. About ¼ of trans women (people assigned male at birth but with a female gender identity) have undergone vaginoplasty (surgical creation of a neovagina, typically using penile and scrotal skin) and while symptoms, such as malodor, discharge and irritation are commonly reported, little is known about the underlying pathophysiology. The vaginal microbiome in cis women is a critical determinant of health, yet our understanding of the composition and role of microbiota colonizing the neovagina is scarce and completely lacking for immune outcomes. In trans men, vaginectomy is rare (<2%), however an estimated 69% use gender-affirming testosterone therapy. Masculinizing hormone therapy has significant effects on the vaginal epithelium that result in reduced cellular proliferation and glycogen production. This is similar to vaginal atrophy observed during menopause in cis women, which affects the vaginal microbiota and has negative impacts on sexual health and quality of life. Little information is available on the relationship between vaginal microbiota and inflammation and the development of vaginal atrophy and symptoms in trans men. Given these unknowns, the short-term goals of this research are to better understand the causes of genital symptoms and the impact of these symptoms on sexual health and quality of life in trans women and men. We will achieve these goals establishing the TransBiota study and characterizing the immunological and microbiological vaginal microenvironments in trans women and men (Aim 1) and then testing for correlations between the vaginal microenvironment and patient characteristics, including sexual behaviors and hygiene practices (vaginal exposures), and importantly vaginal symptoms (Aim 2). We will implement an innovative home-based sampling strategy and an innovative statistical strategy that affords scientific rigor by correcting for experimental errors associated with microbiome analyses. Understanding the vaginal microenvironment and its role in sexual health in trans women and men is an important prerequisite to the development of clinical best- practices, which are currently lacking. The long-term goals of this study are to improve vaginal care for trans women and men and to ultimately leverage this information for the development of novel interventions to achieve optimal vaginal health and protection.

概括跨性别者（跨性别）个人是那些具有不符合其出生分配的性别认同的人性别。 2016年，美国约有140万成年人被确定为变性人。性别肯定的骑术或接受性别疗法的生殖器手术。对性健康筛查和管理的方法，以及获得性传播的风险该人群中其他生殖器病理的感染或发展。 NIH因此确定了一个专业需要创新研究，以解决医学，社会学，心理和结构性原因以及跨性别和性别不合格身份的后果。大约1/4的跨性别女性（分配的人）出生时男性，但具有女性性别认同）进行了阴道成形术（外科医学创造了新诺沃吉纳，通常使用阴茎和阴皮皮肤），而符号（例如Malodor，放电和刺激）是通常报道的是，关于潜在的病理生理学知之甚少。顺式女性的阴道微生物组是健康的关键决定者，但是我们对菌群殖民的组成和作用的理解 Neovagina稀缺，完全缺乏免疫结果。在跨性别男性中，阴道切除术很少见（<2％），但是，估计有69％的人使用性别肯定的睾丸激素治疗。男性化的马龙治疗对阴道上皮的显着影响，导致细胞增殖和糖原产生降低。这类似于在CIS妇女的绝经期间观察到的阴道萎缩，这会影响阴道菌群并对性健康和生活质量产生负面影响。关于关系的信息很少在阴道微生物群和注射以及跨性别的阴道萎缩和症状之间的发展之间男人。鉴于这些未知数，这项研究的短期目标是更好地了解生殖器的原因症状和这些症状对跨性别男女的性健康和生活质量的影响。我们将实现这些目标，以建立跨性别研究并表征免疫学和跨性别女性和男性的微生物阴道微环境（AIM 1），然后测试相关性在阴道微环境和患者特征之间，包括性行为和卫生实践（阴道暴露），重要的是阴道症状（AIM 2）。我们将实施创新以家庭为基础的抽样策略和创新的统计策略，该策略通过纠正来提供科学严格与微生物组分析相关的实验误差。了解阴道微环境及其跨性别男女在性健康中的作用是发展临床最佳的重要前提。目前缺乏的实践。这项研究的长期目标是改善跨性别的阴道护理男女，最终利用这些信息来开发新的干预措施以实现最佳的阴道健康和保护。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

High-resolution functional description of vaginal microbiomes in health and disease.

阴道微生物组在健康和疾病中的高分辨率功能描述。

DOI：
10.1101/2023.03.24.533147
发表时间：
2023
期刊：
bioRxiv : the preprint server for biology
影响因子：
0
作者：
Holm,JohannaB;France,MichaelT;Gajer,Pawel;Ma,Bing;Brotman,RebeccaM;Shardell,Michelle;Forney,Larry;Ravel,Jacques
通讯作者：
Ravel,Jacques

Towards a deeper understanding of the vaginal microbiota.

DOI：
10.1038/s41564-022-01083-2
发表时间：
2022-03
期刊：
Nature microbiology
影响因子：
28.3
作者：
France M;Alizadeh M;Brown S;Ma B;Ravel J
通讯作者：
Ravel J

Complete Genome Sequence of Lactobacillus crispatus CO3MRSI1.

卷曲乳杆菌 CO3MRSI1 的完整基因组序列。

DOI：
10.1128/mra.01538-18
发表时间：
2019
期刊：
Microbiology resource announcements
影响因子：
0.8
作者：
McComb,Elias;Holm,Johanna;Ma,Bing;Ravel,Jacques
通讯作者：
Ravel,Jacques

Cigarette smoking is associated with an altered vaginal tract metabolomic profile.

DOI：
10.1038/s41598-017-14943-3
发表时间：
2018-01-16
期刊：
Scientific reports
影响因子：
4.6
作者：
Nelson TM;Borgogna JC;Michalek RD;Roberts DW;Rath JM;Glover ED;Ravel J;Shardell MD;Yeoman CJ;Brotman RM
通讯作者：
Brotman RM

The Effect of Gender-Affirming Medical Care on the Vaginal and Neovaginal Microbiomes of Transgender and Gender-Diverse People.