CE: Animal Core

CE：动物核心

• 批准号: 7450247
• 负责人: FREDERICK F LANG
• 金额: $ 12.02万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7450247
• 关键词: Animal Disease Models; Animal Experiments; Animal Model; Animals; Behavior; Biological; Brain Neoplasms; Cancer Biology; Cell Line; Core Facility; Doctor of Philosophy; Epidermal Growth Factor Receptor; Gifts; Glioma; Goals; Grant; Human; Investigation; Malignant neoplasm of brain; Modeling; Reproduction spores; Research; Resistance; Solid Neoplasm; Transplantation; University of Texas M D Anderson Cancer Center; Work; Xenograft Model; Xenograft procedure; base; cancer stem cell; clinical efficacy; implantation; in vivo; research study; tumor; tumor xenograft

Investigations into cancer biology and therapy are best carried out in the in vivo setting in animal models of the disease. The ability to reach meaningful conclusions from animal studies, such as accurately predicting biological tumor behavior or clinical efficacy of an investigational agent, is greatly enhanced when uniform, readily reproducible animal models are used. In this context, The University of Texas M. D. Anderson Cancer Center (UTMDACC) SPORE in SPORE in Brain Cancer will rely on the Animal Core facility, which was developed over 5 years ago by the Co-Directors, and whose goal is to provide consistent and reproducible heterotopic and orthotopic animal models of brain tumors. The majority of projects in this SPORE grant rely on intracranial (orthotopic) implantation of established, adherent gliomas cell lines, which are molecularly well characterized. However, we have also developed orthotopic xenografts of human brain cancer stem cells, which recent work has indicated are important for the genesis of most solid tumors, including high-grade gliomas. Finally, the Core has obtained and maintains EGFR+ glioma xenografts (gift of C. David James, Ph.D.), which require serial transplant from animal to animal in order to preserve the EGFR+ status of these tumors. Therefore, the Specific Aims of the Animal Core are: Aim 1: Provide support for animal experiments using heterotopic and orthotopic models derived from commercially available human glioma cell lines. Aim 2: Provide support for the animal experiments using orthotopic models derived from brain cancer stem cells. Aim 3: Maintain EGFR+ tumors by serial transplant, exclusively in animals, and provide support for animal experiments using these EGFR+ tumor xenografts. Aim 4: Maintain reference and working stock banks of all cell lines.

对癌症生物学和治疗的研究最好在动物模型的体内环境中进行 这种疾病。从动物研究中得出有意义的结论的能力，例如准确预测 当研究药物的生物肿瘤行为或临床疗效均一时，会大大增强， 使用易于重复的动物模型。在此背景下，德克萨斯大学 M. D. 安德森 癌症中心 (UTMDACC) SPORE 脑癌 SPORE 将依赖动物核心设施，该设施 是由联合董事在 5 年前开发的，其目标是提供一致和 可重复的异位和原位脑肿瘤动物模型。这一领域的大部分项目 SPORE 资助依赖于已建立的贴壁神经胶质瘤细胞系的颅内（原位）植入，这 具有良好的分子特征。然而，我们还开发了人类原位异种移植物 脑癌干细胞，最近的研究表明，它对于大多数实体的发生很重要 肿瘤，包括高级别神经胶质瘤。最终，Core获得并维持EGFR+胶质瘤 异种移植（由 C. David James 博士捐赠），需要从动物到动物进行连续移植，以便 保留这些肿瘤的 EGFR+ 状态。因此，动物核心的具体目标是： 目标 1：使用衍生自异位和原位的模型为动物实验提供支持 市售的人神经胶质瘤细胞系。 目标2：为使用脑癌原位模型的动物实验提供支持 干细胞。 目标 3：仅在动物中通过系列移植维持 EGFR+ 肿瘤，并为 使用这些 EGFR+ 肿瘤异种移植物进行动物实验。 目标 4：维护所有细胞系的参考和工作库存库。