MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

分子分析细胞成像 (MACI) 核心

• 批准号: 10077912
• 负责人: WILLARD M FREEMAN
• 金额: $ 36.3万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077912
• 关键词: Aging; Animal Model; Applications Grants; Area; Binding; Biochemical; Bioinformatics; Biological; Cells; ChIP-seq; Chromatin; Complement; Consultations; Core Facility; Creativeness; DNA Methylation; DNA-Binding Proteins; Data; Data Analyses; Data Set; Detection; Development; Disease; Epigenetic Process; Experimental Designs; Fostering; Gene Expression; Gene Expression Profiling; Gene Expression Regulation; Gene Proteins; Genes; Geroscience; Goals; Growth; Health; Health Sciences; Heterogeneity; Human Resources; Image; Image Analysis; Imaging technology; Individual; Investigation; Label; Laboratories; Light; Link; Microscopy; Mitochondria; Modality; Molecular; Molecular Analysis; Nucleic Acids; Oklahoma; Pathway interactions; Preparation; Process; Program Development; Protein Isoforms; Proteins; Publishing; Regulation; Research; Research Design; Research Personnel; Resources; Role; Scientist; Services; Shock; Structure; Tissue Sample; Training; Training Technics; Transcript; Transmission Electron Microscopy; age related; cellular imaging; design; epigenetic regulation; experience; heteroplasmy; imaging approach; imaging capabilities; imaging study; innovation; insight; instrumentation; interest; knowledge base; molecular imaging; programs; protein expression; recruit; single-cell RNA sequencing; skills; tissue preparation; tool; transcription factor; transcriptome; transcriptome sequencing

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a range of instrumentation, services, and training for rigorous analysis of structure and function through gene expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with extensive scientific experience in these areas who already provide collaborative assistance to individual investigators and have significant experience operating core resources. The MACI Core will offer a range of technical services, and also expert advice in study design, experimental approaches, data analysis and interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA- Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing (ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies, the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and transmission electron microscopy services. A key service will be to help our PJIs and other investigators to develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training seminars and project-specific training will expand the knowledge base and capabilities of Geroscience researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research and foster independence, innovation, and creativity of the individual research programs.

基本的GEROSCIENCE研究需要分析基因和蛋白质调节，表达和细胞 本土化。先进的分子分析和成像技术为研究人员提供了一套强大的工具 为了他们的学习，但是仪器，经验和技能充分利用这些工具通常远远超出了 单个实验室的能力。分子分析和细胞成像（MACI）核心将提供 通过基因对结构和功能进行严格分析的仪器，服务和培训范围 表达，基因调节和成像研究。这样，Maci核心将提高对 健康和疾病的分子和细胞年龄相关的变化。 Maci核心将雇用人员 这些领域的广泛科学经验已经为个人提供协作帮助 调查人员并具有丰富的经验经营核心资源。 MACI核心将提供一系列 技术服务以及研究设计，实验方法，数据分析和 解释，赠款提案准备和培训。 MACI核心将提供高级分子 基因表达和调节的分析（AIM 1），包括高吞吐量单细胞RNA-Seq（HT-SCRNA- SEQ）特异性RNA-SEQ基因表达，高通量QPCR，染色质免疫沉淀测序 （chip-seq）用于DNA结合蛋白。 MACI核心的AIM 2是提供高级的，特定于应用的组织 制备，细胞标记，成像和图像分析服务和培训。对于细胞定位研究， MacI核心将提供组织制备和切片以及光线，共焦，落荧光和 传输电子显微镜服务。关键服务将是帮助我们的PJI和其他调查人员 开发先进的，特异性的细胞标记和成像方法。 MACI核心的目标3是提供 培训以及高级分子和细胞数据分析服务。从核酸的分离和 组织制备和分割生物信息学和图像分析，双月训练的组合 研讨会和特定项目培训将扩大GEROSCIENCE的知识库和能力 研究人员。还将提供有关实验设计，分析和赠款建议的咨询。这 Maci Core以及合作伙伴核心将有助于Geroscience Research的发展和持续增长 以及促进独立，创新和个人研究计划的创造力。