MOLECULAR ANALYSIS CELLULAR IMAGING (MACI) CORE

分子分析细胞成像 (MACI) 核心

基本信息

批准号：
10536646
负责人：
WILLARD M FREEMAN
金额：
$ 41.2万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-02-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10536646
关键词：
Aging Animal Model Applications Grants Area Binding Biochemical Bioinformatics Biological Cell Separation ChIP-seq Chromatin Complement Consultations Core Facility Creativeness DNA Methylation DNA-Binding Proteins Data Data Analyses Data Set Detection Development Disease Epigenetic Process Experimental Designs Fostering Gene Expression Gene Expression Profiling Gene Expression Regulation Genes Geroscience Goals Growth Health Health Sciences Heterogeneity Human Resources Image Image Analysis Imaging technology Individual Investigation Label Laboratories Light Link Microscopy Mitochondria Modality Molecular Molecular Analysis Nucleic Acids Oklahoma Pathway interactions Preparation Process Program Development Protein Isoforms Proteins Publishing Regulation Research Research Design Research Personnel Resources Role Scientist Services Shock Structure Techniques Tissue Sample Training Transcript Transmission Electron Microscopy age related cellular imaging design epigenetic regulation experience heteroplasmy imaging approach imaging capabilities imaging study innovation insight instrumentation interest knowledge base molecular imaging programs protein expression recruit single-cell RNA sequencing skills tissue preparation tool transcription factor transcriptome transcriptome sequencing

项目摘要

Basic Geroscience research requires the analysis of gene and protein regulation, expression, and cellular localization. Advanced molecular analyses and imaging technologies offer investigators a powerful set of tools for their studies, but the instrumentation, experience, and skills to fully exploit these tools are often far beyond the capability of a single laboratory. The Molecular Analysis and Cellular Imaging (MACI) Core will provide a range of instrumentation, services, and training for rigorous analysis of structure and function through gene expression, gene regulation, and imaging studies. In this way, the MACI Core will advance understanding of molecular and cellular age-related changes in health and disease. The MACI Core will employ personnel with extensive scientific experience in these areas who already provide collaborative assistance to individual investigators and have significant experience operating core resources. The MACI Core will offer a range of technical services, and also expert advice in study design, experimental approaches, data analysis and interpretation, grant proposal preparation and training. The MACI Core will provide advanced molecular analyses of gene expression and regulation (Aim 1) including high throughput single-cell RNA-Seq (HT-scRNA- Seq) strand-specific RNA-Seq gene expression, high-throughput qPCR, chromatin immunoprecipitation sequencing (ChIP-Seq) for DNA binding proteins. Aim 2 of the MACI Core is to provide advanced, application-specific tissue preparation, cellular labeling, imaging, and image analysis services and training. For cellular localization studies, the MACI Core will offer tissue preparation and sectioning along with light, confocal, epifluorescence, and transmission electron microscopy services. A key service will be to help our PJIs and other investigators to develop advanced, lab-specific cellular labeling and imaging approaches. Aim 3 of the MACI Core is to provide training and advanced molecular and cellular data analysis services. Ranging from nucleic acid isolation and tissue preparation and sectioning to bioinformatics and image analysis, a combination of bimonthly training seminars and project-specific training will expand the knowledge base and capabilities of Geroscience researchers. Consultation on experimental design, analysis, and grant proposals will also be provided. The MACI Core, along with partner Cores, will aid the development and continued growth of Geroscience research and foster independence, innovation, and creativity of the individual research programs.

基础老年科学研究需要分析基因和蛋白质调控、表达和细胞本土化。先进的分子分析和成像技术为研究人员提供了一套强大的工具但充分利用这些工具的仪器、经验和技能往往远远超出了他们的研究范围单个实验室的能力。分子分析和细胞成像 (MACI) 核心将提供通过基因对结构和功能进行严格分析的一系列仪器、服务和培训表达、基因调控和成像研究。通过这种方式，MACI 核心将促进对分子和细胞年龄相关的健康和疾病变化。 MACI 核心将雇用以下人员：在这些领域拥有丰富的科学经验，他们已经为个人提供了协作帮助调查员并拥有运营核心资源的丰富经验。 MACI 核心将提供一系列技术服务，以及研究设计、实验方法、数据分析和研究方面的专家建议解释、赠款提案准备和培训。 MACI Core 将提供先进的分子基因表达和调控分析（目标 1），包括高通量单细胞 RNA 测序 (HT-scRNA- Seq) 链特异性 RNA-Seq 基因表达、高通量 qPCR、染色质免疫沉淀测序 (ChIP-Seq) 用于 DNA 结合蛋白。 MACI 核心的目标 2 是提供先进的、特定于应用的组织制备、细胞标记、成像以及图像分析服务和培训。对于细胞定位研究， MACI Core 将提供组织制备和切片以及光、共焦、落射荧光和透射电子显微镜服务。一项关键服务是帮助我们的 PJI 和其他调查人员开发先进的、实验室特定的细胞标记和成像方法。 MACI 核心的目标 3 是提供培训和先进的分子和细胞数据分析服务。从核酸分离到组织准备和切片到生物信息学和图像分析，每两个月一次的培训相结合研讨会和特定项目的培训将扩大老年科学的知识库和能力研究人员。还将提供有关实验设计、分析和资助提案的咨询。这 MACI Core 与合作伙伴 Cores 将帮助老年科学研究的发展和持续增长培养个人研究项目的独立性、创新性和创造力。