Plasmonic Glycan Arrays for Highly Sensitive Detection of Glycan-binding Proteins

用于高灵敏度检测聚糖结合蛋白的等离子聚糖阵列

• 批准号: 10081148
• 负责人: XICHUN ZHOU
• 金额: $ 22.29万
• 依托单位: VITAN-BIOTECH, LLC
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10081148
• 关键词: Affinity; Antibodies; Binding; Binding Proteins; Biological; Biological Assay; Biological Markers; Biological Process; Biology; Biomedical Research; Biotechnology; Cancer Biology; Charge; Chemistry; Clinical; Complex; Core Protein; Detection; Development; Diagnostic; Disease; Drug Targeting; Dyes; Environment; Event; Film; Fluorescence; Genomics; Glass; Glycobiology; Glycoproteins; Glycosaminoglycans; Goals; Growth Factor; Health; Immobilization; Immunoassay; Inflammation; Instinct; Knowledge; Label; Lectin; Ligands; Malignant Neoplasms; Mass Spectrum Analysis; Measures; Methods; Modeling; Nanotechnology; Neoplasm Metastasis; Phase; Photons; Play; Polysaccharides; Proteins; Proteoglycan; Proteomics; RBL2 gene; Reagent; Reproducibility; Research; Role; Sampling; Signal Transduction; Slide; Small Business Innovation Research Grant; Surface; System; Technology; Therapeutic; Thick; Tissues; Tumor Angiogenesis; Vaccines; Variant; analytical tool; angiogenesis; base; carbohydrate binding protein; carbohydrate structure; cell behavior; cost effective; cyanine dye 5; density; design; fluorophore; high throughput screening; instrumentation; nanorod; nervous system disorder; plasmonics; response; sugar; technology development; technology research and development; tool

Project Summary Exploring the biological information content in glycans represents one of the current focuses of postgenomic research and technology development. Glycan-based high throughput assays (e.g. glycan microarrays) hold great promise for the rapid analysis of carbohydrate binding proteins (CBPs), elucidation of CBP biology, and the development of diagnostics, vaccines, and therapeutics for a number of diseases, including cancer and neurological disorders. As the field of glycobiology grows, the important roles that glycans and glycoproteins play in health are becoming increasingly recognized. However, the “state- of-the-art” glycan arrays have several instinct limitations including poor dynamic range, poor sensitivity, and inability to easily interrogate multiple glycan-protein interactions simultaneously in complex samples. Because protein–glycan interactions are typically of low affinity, and many CBPs and auto-anti-glycan antibodies in clinical samples are generally in the range of fg/mL to pg/mL, the detection sensitivity of current glycan arrays is unable to reach the clinical threshold of many CBPs and auto-anti-glycan antibody biomarkers, especially in the early stages of the diseases when their concentrations in clinical samples very low. The goal of this project is to applied nanotechnology to overcome the major limitations of poor sensitivity and dynamic ranges of current glycan microarrays. We aim to develop and commercialize multi-plasmonic resonant photon enhanced fluorescent (MPREF) glycan array for glycomic research. With this project, we expect to develop ready-to-use products of: 1) the MPREF system include the components of plasmonic substrate and plasmonic fluorophores which will be marketed as glycan array kit, 2) a validated model MPREF glycan array containing 50+ Glycosaminoglycans.

项目摘要 探索聚糖中的生物信息含量代表了当前 基于聚糖的高级研究和技术开发的重点 吞吐量测定（例如Glycan微阵列）对快速分析有很大的希望 碳水化合物结合蛋白（CBP），CBP生物学的阐明和发展 多种疾病，倾斜癌和 神经系统疾病。 糖蛋白在健康中发挥作用，越来越被认可。 “聚糖阵列”具有几个本能的限制，包括动态范围差，差 敏感性，无法轻松询问多种聚糖蛋白相互作用 同时在复杂样品中。 临床样品中的亲和力以及许多CBP和自动抗聚糖抗体一般都在 FG/ML到PG/ML的范围，当前聚糖阵列的检测灵敏度无法达到。 达到许多CBP和自动抗体生物标志物的临床阈值， 尤其是在那时的早期阶段，然后它们在临床样品中的浓度 非常低的目标是应用纳米技术来克服专业 我们的目的是限制敏感性和动态范围 开发和商业化多质子共振光子增强荧光（MPREF） 与该项目一起进行糖研究的聚糖阵列。 产品的产品：1）MPREF系统包括等离子基底物的组成部分和 等离子荧光团将作为聚糖阵列套件销售，2）经过验证的模型 MPREF聚糖阵列，其中包含50多个糖胺聚糖。