Novel 3-dimensional platform for high-throughput glycomics discovery

用于高通量糖组学发现的新型 3 维平台

• 批准号: 7218271
• 负责人: XICHUN ZHOU
• 金额: $ 10.18万
• 依托单位: ADA TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2008-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7218271
• 关键词: 3-Dimensional; Address; Amines; Atomic Force Microscopy; Binding; Bioinformatics; Biological Process; Biomedical Research; Biopolymers; Carbohydrates; Cell Adhesion; Cell Differentiation process; Characteristics; Chemicals; Collaborations; Condition; Development; Event; Film; Glass; Glycoconjugates; Goals; Immobilization; Immune response; Laboratories; Left; Ligands; Marketing; Methods; Microarray Analysis; Microscope; Modeling; Modification; Monitor; Mono-S; Monosaccharides; Neoplasm Metastasis; Noise; Oligosaccharides; Oximes; Performance; Phase; Physical condensation; Polysaccharides; Population; Protein Binding; Protein-Carbohydrate Interaction; Proteins; Proteomics; Range; Research; Role; Signal Transduction; Site; Slide; Small Business Funding Mechanisms; Small Business Innovation Research Grant; Solid; Spottings; Structure; Surface; Surveys; Technology; Testing; Universities; Work; biochip; carbohydrate binding protein; carbohydrate receptor; carbonyl group; frontier; functional group; high throughput analysis; instrument; medical schools; microwave electromagnetic radiation; miniaturize; monolayer; neoplastic cell; novel; novel strategies; programs; sugar; tool; trafficking; two-dimensional

DESCRIPTION (provided by applicant): Glycomics is an emerging field that focuses on studying the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. Carbohydrate microarray technologies represent a new development at the frontier of glycomics. Microarrays of carbohydrates (glycoconjugates, polysaccharides, oligosaccharides and monosaccharides) allow high-throughput, parallel analyses. Parallel analyses greatly facilitate not only surveys of proteins for carbohydrate-binding activities but also elucidation of their ligands. However, there is a lack of technology for the fabrication of high quality carbohydrate microarrays for high-throughput analysis of glycomes because of the difficulty to reliably immobilize populations of chemically and structurally diverse glycans. ADA proposes to develop aminooxy- and hydrazide-functionalized dendrimer monolayer films on solid substrates for covalent, site-specific immobilization of unmodified carbohydrate probes. Miniaturized microwave radiation energy will also be evaluated for quick and convenient immobilization of the polysaccharides on the proposed slide platform. The resulting platforms will allow facile and effective fabrication of carbohydrate microarrays. The proposed novel slide platform will significantly increase the efficiency of glycan analysis and meet the growing needs of glycomics research and biomedical applications. Glycomics study the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. This proposed research seeks to develop a new tool for biologists to significantly increase the efficiency in glycan anaysis and meet the growing needs of glycomics research and biomedical applications.

描述（由申请人提供）：糖果是一个新兴领域，侧重于研究碳水化合物与其他生物聚合物，尤其是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。碳水化合物微阵列技术代表了糖胶体边界的新开发项目。碳水化合物的微阵列（糖缀合物，多糖，寡糖和单糖）允许高通量，平行分析。平行分析不仅极大地促进了蛋白质的碳水化合物结合活性的调查，还可以阐明其配体。但是，缺乏用于制造高质量碳水化合物微阵列的技术，无法对糖果进行高通量分析，因为难以可靠地固定化学和结构上多样的聚糖种群。 ADA建议在固体底物上开发氨基和氢化官官能化的树枝状聚合物单层膜，以使未修改的碳水化合物探针共价，位点特异性固定化。还将评估微型微波辐射能，以便在提议的幻灯片平台上快速且方便地固定多糖。最终的平台将允许轻松地制造碳水化合物微阵列。拟议的新型幻灯片平台将显着提高聚糖分析的效率，并满足糖研究和生物医学应用的不断增长的需求。糖基因研究碳水化合物与其他生物聚合物，尤其是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。这项拟议的研究旨在为生物学家开发一种新的工具，以显着提高聚糖anaysis的效率，并满足糖研究和生物医学应用的不断增长的需求。