Consequences of developmental defects in somatosensory map formation

体感图形成发育缺陷的后果

基本信息

批准号：
8722646
负责人：
Reha S Erzurumlu
金额：
$ 35.1万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-02-01 至 2019-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8722646
关键词：
Affect Animals Auditory system Axon Behavior Behavioral Behavioral Mechanisms Bilateral Biology Body Image Brain Calcium Cell Nucleus Cells Cerebral cortex Cerebral hemisphere Communication Congenital Abnormality Contralateral Coupled Defect Development Dyes Electrophysiology (science)Electroporation Esthesia FOS gene Face Gene Deletion Gene Mutation Head Human Human Genetics Image Indium Injury Ipsilateral Label Lead Light Maps Microscopy Molecular Motor Motor Pathways Movement Mus Mutation Neocortex Nerve Neurons Ocular dominance columns Outcome Pathway interactions Pattern Perception Phenotype Pontine structure Preparation Property Sensory Sensory Process Sensory Receptors Side Site Somatosensory Cortex Synapses System Techniques Testing Thalamic structure Transgenic Mice Vibrissae Vision Visual Cortex Visual system structure Weight Work afferent nerve awake barrel cortex brain pathway density developmental genetics in utero information processing mouse model postsynaptic public health relevance relating to nervous system research study response sensorimotor system sensory cortex skills somatosensory two-photon voltage

项目摘要

Topographic representation of the sensory space in the brain is essential for sensory information processing and perception. The somatosensory and motor cortical maps in each hemisphere represent the contralateral body and the face. This is due to midline crossing of the ascending (sensory) and descending (motor) pathways at the level of the medulla or the pons. Genetic and developmental defects in midline crossing or injury at the crossing site severely affect sensory-motor information processing and actions in both animals and humans. In this proposal we use a region-specific gene deletion mouse model to study the consequences of partial crossing of the ascending somatosensory face pathway. Midline crossing defects in this mouse leads to bilateral face representation in the thalamus and subsequently in the somatosensory cortex. We will use this mouse model to investigate (a) morphological and electrophysiological properties of the pre and postsynaptic elements in the "bifacial" cortical map; (b) altered thalamocortical and corticocortical connectivity patterns in response to bilateral face representation; (c) behavioral consequences of this genetic mutation. A combination of molecular, morphological, electrophysiological, voltage-sensitive dye imaging and behavioral techniques will be used to elucidate mechanisms underlying the functional organization and behavioral manifestations of developmental injury-related or genetic defects in ascending somatosensory pathways.

大脑中感觉空间的地形表示对于感觉信息至关重要处理和感知。每个半球中的体感和运动皮质图代表对侧身体和脸部。这是由于上升（感觉）和下降的中线交叉（电动机）延髓或pon的途径。中线遗传和发育缺陷穿越现场的穿越或伤害严重影响感觉运动信息处理和两者的行动动物和人类。在此提案中，我们使用特定于区域的基因缺失小鼠模型来研究上升的体感面部途径的部分穿越的后果。中线交叉缺陷该鼠标导致丘脑中的双侧面表示，随后在体感中皮质。我们将使用该小鼠模型来研究（a）（a）（a）的形态和电生理特性 “双面”皮质图中的突触前和突触后元素；（b）改变丘脑皮层和皮质皮质的改变响应双侧面部表示的连通性模式；（c）这种遗传的行为后果突变。分子，形态学，电生理，电压敏感染料成像和行为技术将用于阐明功能组织和发育损伤相关或遗传缺陷的行为表现在上升的体感中途径。