The function of small RNAs in the nitrogen response

小RNA在氮反应中的功能

基本信息

批准号：
7544970
负责人：
Gloria CORUZZI
金额：
$ 5.05万
依托单位：
NEW YORK UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-01-01 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7544970
关键词：
Address Affect Agriculture Amino Acids Animals Arabidopsis Area Arts Assimilations Bioinformatics Biology Biotechnology Carbon Cells Chile Classification Collaborations Computers DNA Sequence Data Data Set Databases Delaware Development Developmental Gene Dose Ectopic Expression Experimental Designs Frequencies Funding Gene Expression Gene Expression Regulation Gene Targeting Genes Genetic Screening Genome Genomics Goals Grant Growth and Development function Health Human Imagery Informatics Institutes Knowledge Letters Light Logic Mediating Metabolic MicroRNAs Microarray Analysis Modeling Molecular Nitrogen Northern Blotting Organism Parents Pathway interactions Phenotype Plants Play Post-Transcriptional Regulation Protocols documentation Regulation Research Role Sampling Seedling Seeds Signal Transduction Small RNA Source South America System Technology Testing Time Transcript United States National Institutes of Health Work base combinatorial computerized tools data integration design gene interaction genome-wide improved in vivo insight knockout gene metabolomics molecular phenotype mutant network models novel nutrition positional cloning programs research study response tool

项目摘要

DESCRIPTION (provided by applicant): Our long-term goal is to understand how nitrogen (N) signaling controls plant N-assimilation, growth and development. Identifying key components involved in regulating N-assimilation offers opportunities for improving N-use efficiency in plants or modifying the amino acid content of seeds, which are important issues for health, agriculture and human nutrition. The experiments in this proposal should provide insight into how plants sense and respond to N at the molecular level, specifically through the post transcriptional control of gene expression by microRNAs (miRNAs) and other small RNAs (sRNAs). Identifying the sRNAs involved in regulation of gene networks in response to N-treatment could identify mechanisms which may be altered to optimize N-assimilation via the ectopic expression or misexpression of specific sRNAs. It is now clear that miRNAs are key components of regulatory networks in plants and animals, but their role in mediating the N- response of target genes has not been addressed. We propose to (1) Identify the miRNAs and other sRNAs whose expression is regulated by N-treatments; (2) Identify the gene networks that are controlled by these N- regulated sRNAs; and (3) Understand the functional role of sRNAs in mediating the N-response of target genes in the network. Our experimental approach will be to isolate sRNAs from plants treated transiently with inorganic N sources, sequence the sRNAs using 454 technology, and compare the frequency of transcripts in these samples with the frequency in samples from mock-treated plants. We will incorporate known and novel sRNA:RNA interactions into our existing gene network models to identify subnetworks that are controlled by N- responsive sRNAs. We will use reverse genetic and genomic approaches to evaluate the role of specific sRNAs in the plant response to N-treatments. The combination of bioinformatic and genomic approaches for functional studies in this project performed in collaboration with Dr. Coruzzi's group, will greatly strengthen the research capability of Dr. Gutiirrez research program in Chile and South America. Expertise in these areas is essential for competitive research programs in the post-genomic era, and it is an underdeveloped area of research in Chile and South America. Most of the research will be carried out in Chile at P. Universidad Catslica de Chile in collaboration with Dr. Rodrigo Gutiirrez, as an extension of NIH grant #2R01GM032877- 21 to Dr. Gloria M. Coruzzi. This proposal should provide insight into how plants sense and respond to nitrogen at the molecular level. Identifying key components involved in regulating nitrogen assimilation offers opportunities for improving nitrogen-use efficiency in plants or modifying the amino acid content of seeds, which are important issues for health, agriculture and human nutrition.

描述（由申请人提供）：我们的长期目标是了解氮 (N) 信号如何控制植物氮同化、生长和发育。识别参与调节氮同化的关键成分为提高植物氮利用效率或改变种子氨基酸含量提供了机会，这对于健康、农业和人类营养来说是重要问题。本提案中的实验应深入了解植物如何在分子水平上感知和响应氮，特别是通过 microRNA (miRNA) 和其他小 RNA (sRNA) 对基因表达进行转录后控制。识别响应氮处理而参与基因网络调节的 sRNA 可以识别可通过特定 sRNA 的异位表达或错误表达来改变以优化氮同化的机制。现在已经清楚，miRNA 是动植物调控网络的关键组成部分，但它们在介导靶基因 N 响应中的作用尚未得到解决。我们建议：（1）鉴定表达受N处理调节的miRNA和其他sRNA； (2) 识别受这些 N 调控 sRNA 控制的基因网络； (3) 了解 sRNA 在介导网络中靶基因 N 响应中的功能作用。我们的实验方法是从用无机氮源短暂处理的植物中分离 sRNA，使用 454 技术对 sRNA 进行测序，并将这些样品中的转录物频率与模拟处理植物的样品中的频率进行比较。我们将把已知的和新颖的 sRNA:RNA 相互作用纳入我们现有的基因网络模型中，以识别由 N 响应 sRNA 控制的子网络。我们将使用反向遗传学和基因组方法来评估特定 sRNA 在植物对 N 处理的反应中的作用。该项目与 Coruzzi 博士的团队合作，将生物信息学和基因组方法相结合进行功能研究，将大大加强 Gutiirrez 博士在智利和南美洲的研究项目的研究能力。这些领域的专业知识对于后基因组时代的竞争性研究项目至关重要，而这在智利和南美洲是一个不发达的研究领域。大部分研究将在智利 P. Universidad Catslica de Chile 与 Rodrigo Gutiirrez 博士合作进行，作为 NIH 拨款 #2R01GM032877-21 授予 Gloria M. Coruzzi 博士的延伸。该提案应该可以深入了解植物如何在分子水平上感知和响应氮。识别参与调节氮同化的关键成分为提高植物氮利用效率或改变种子氨基酸含量提供了机会，这对于健康、农业和人类营养来说是重要问题。