Microbial Genomics Biomedical Core

微生物基因组学生物医学核心

基本信息

批准号：
10022313
负责人：
Anne-Catrin Uhlemann
金额：
$ 18.5万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-24 至 2021-09-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10022313
关键词：
Acute Adherence Affect Area Bacterial Infections Bioinformatics CRISPR/Cas technology Clinical Communities Congenital Abnormality Consultations Core Facility Custom Data Analyses Disease Disease model Education Ensure Environment Event Experimental Designs Gene Expression Genes Genetic Genetic Polymorphism Genome Genomic medicine Genomics Genotype Goals Heme Homeostasis Human Individual Infection prevention Investigation Knock-in Knock-out Laboratories Life Style Medical center Medicine Mentorship Metabolic Metabolic Pathway Metagenomics Methods Microbial Genetics Microbiology Mission Modernization Mus Mutation National Institute of Diabetes and Digestive and Kidney Diseases Nucleic Acids Oligonucleotide Primers Organism Participant Pathogenesis Pathogenicity Pathology Patients Phenotype Play Policies Property RNA Reproducibility Research Research Design Research Personnel Research Project Grants Resource Sharing Resources Ribosomal RNA Role Sample Size Sampling Services Students Symptoms Technology Testing Time Universities Urinary tract infection Urine Urologic Diseases Urology Urothelium Vesico-Ureteral Reflux Virulence Work base biobank cohort comparative genomics design experimental study genome analysis genome sequencing genomic tools human subject improved individual variation instrumentation interest lectures member metagenome metagenomic sequencing microbial microbial genomics microbiome microbiome analysis mouse model mutant next generation next generation sequencing novel pathogen pathogen genomics pathogenic microbe precision medicine programs response sample collection transcriptome sequencing urinary urologic whole genome

项目摘要

MICROBIAL GENOMICS BIOMEDICAL CORE (MGBC): PROJECT SUMMARY/ABSTRACT The mission of the Columbia University O’Brien Urology Research Center is to identify genetic factors and congenital malformations that predispose individuals to UTI, such as vesicoureteral reflux, to explore host- bacterial metabolic pathways, such as heme, that enable or prevent infection. To achieve this goal, the Microbial Genomics and Biomedical Core (MGBC) will provide high quality services for biobanking, extended bacterial culture, microbial genomics (microbiome analyses, comparative genomics, RNASeq), microbial genetics and consultation for study design. The MGBC will enable Columbia O’Brien Center investigators to have access to specialized genomic technologies, coordinated expert consultations and improved rigor and reproducibility in genomics and precision medicine. These services are specifically designed to meet the needs of the Research Projects of the Columbia O’Brien Center. Moreover, the MBMC will serve as a national resource for members of other George M. O'Brien Cooperative Research Centers and other NIDDK Urologic Research Programs. Additionally, the MGBC will work closely with the Administrative Core to ensure adherence to all fiscal, administrative, and resource sharing policies. The MGBC will also contribute to the goals of the Educational Enrichment and Opportunity Pool Programs through lectures, student mentorship, and guidance on experimental design related to microbial genomics for Opportunity Pool awardees. The MBMC will accomplish these goals through the following aims: 1) Establish a urine biorepository from clinically well-defined patients with different UTI symptoms; 2) Provide Microbiome and metagenome analyses; 3) Perform extended urine culture for organism identification; 4) Facilitate whole genome sequencing of dominant UTI pathogenic clones; 5) Determine gene expression by RNAseq and quantitative real-time PCR; 6) Generate bacterial mutants; and 7) Provide consultations and support of study design for the Research Projects.

微生物基因组学生物医学核心 (MGBC)：项目摘要/摘要哥伦比亚大学奥布莱恩泌尿学研究中心的使命是识别遗传因素和导致个体易患尿路感染的先天性畸形，例如膀胱输尿管反流，以探索宿主为了实现这一目标，微生物代谢途径，例如血红素，能够实现或预防感染。基因组学和生物医学核心（MGBC）将为生物样本库、扩展细菌提供高质量服务培养、微生物基因组学（微生物组分析、比较基因组学、RNASeq）、微生物遗传学和 MGBC 将使哥伦比亚奥布莱恩中心的研究人员能够获得研究设计的咨询。专门的基因组技术、协调的专家咨询以及改进的结果和再现性这些服务是专门为满足研究的需求而设计的。此外，MBMC 将作为哥伦比亚奥布莱恩中心成员的国家资源。其他 George M. O'Brien 合作研究中心和其他 NIDDK 泌尿学研究项目。此外，MGBC 将与行政核心密切合作，以确保遵守所有财政、 MGBC 还将为实现教育目标做出贡献。通过讲座、学生指导和实验指导进行丰富和机会池计划为机会库获奖者提供与微生物基因组学相关的设计将实现这些目标。通过以下目标：1）建立临床明确的不同患者的尿液生物样本库尿路感染症状；2) 提供微生物组和宏基因组分析；3) 进行长期尿液培养；生物体鉴定；4) 促进显性 UTI 致病克隆的全基因组测序；通过 RNAseq 和定量实时 PCR 进行基因表达；6) 生成细菌突变体；7) 提供研究项目研究设计的咨询和支持。