Microbial Genomics Biomedical Core

微生物基因组学生物医学核心

• 批准号: 10297544
• 负责人: Anne-Catrin Uhlemann
• 金额: $ 22.79万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-24 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10297544
• 关键词: 16S ribosomal RNA sequencing; Adherence; Affect; Antimicrobial Resistance; Area; Bacteria; Bacterial Genes; Bacterial Infections; Benign; Bioinformatics; CRISPR/Cas technology; Clinical; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; Complement; Congenital Abnormality; Consultations; Disease; Disease model; Drug resistance; Education; Ensure; Environment; Event; Experimental Designs; Future; Genes; Genetic; Genetic Polymorphism; Genome; Genomic medicine; Genomics; Goals; Heme; Hi-C; Homeostasis; Human; Individual; Infection; Infection prevention; Investigation; Laboratories; Life Style; Medical center; Mentorship; Metabolic; Metabolism; Metagenomics; Methods; Microbe; Microbial Genetics; Microbiology; Mission; Modernization; Molecular Biology; Multi-Drug Resistance; Mus; Mutation; National Institute of Diabetes and Digestive and Kidney Diseases; New York City; Nucleic Acids; Organism; Participant; Pathogenesis; Pathogenicity; Pathology; Pathway interactions; Patients; Pattern; Phenotype; Plasmids; Play; Policies; Preparation; Public Health; Reproducibility; Research; Research Design; Research Personnel; Research Project Grants; Resource Sharing; Resources; Ribosomal RNA; Role; Sample Size; Sampling; Services; Site; Students; Symptoms; System; Technology; Testing; Universities; Urinary tract infection; Urine; Urologic Diseases; Urology; Uropathogen; Urothelium; Vesico-Ureteral Reflux; base; biobank; cohort; comparative; comparative genomics; design; experience; experimental study; functional genomics; genome analysis; genome sequencing; human subject; improved; individual variation; instrument; interest; knockout gene; lectures; member; metagenome; microbial genomics; microbiome; microbiome analysis; mouse model; mutant; next generation; next generation sequencing; novel; outreach; pathogen; precision medicine; programs; prospective; response to injury; sample collection; symptomatology; tool; urinary; urologic; whole genome

MICROBIAL GENOMICS BIOMEDICAL CORE (MGBC): PROJECT SUMMARY/ABSTRACT The mission of the Columbia University O’Brien Urology Research Center is to identify genetic factors and congenital malformations, such as vesicoureteral reflux, that predispose individuals to urinary tract infections (UTI) and to explore host-bacterial metabolic processes, such as heme pathways, that enable or prevent infection. UTI represent one of the most common infections, affecting 150 million people per year worldwide. In support of the goals of the Columbia O’Brien Urology Research Center the Microbial Genomics and Biomedical Core (MGBC) will provide high quality services for biobanking, extended bacterial culture, microbial genomics (microbiome analyses, comparative genomics), microbial genetics, consultation for study design and outreach for novel investigations into the pathogenesis of urinary tract infections. The MGBC will enable Columbia O’Brien Center investigators to have access to specialized genomic technologies and coordinated expert consultations for improved rigor and reproducibility in genomics and precision medicine. In recognition of the increasing impact of antimicrobial resistance in urinary tract infections the MGBC has established a biorepository of multi-drug resistant UTI isolates. The services and biorepository are specifically designed to meet the needs of two of the Research Projects of the Columbia O’Brien Center and Opportunity Pool Awardees and will be available to the wider urology research community. The MGBC will accomplish these goals through the following aims: 1) Expand a biorepository of multi-drug resistant UTI isolate to multiple sites across New York City, establish a urine biorepository from clinically well-defined patients with different UTI symptoms and perform extended urine culture for organism identification; 2) Provide Microbiome and metagenome analyses and Hi-C/MetaPore-C sequencing; 3) Facilitate whole genome sequencing of dominant UTI pathogenic clones to elucidate bacterial determinants of UTI symptomatology; 4) Generate bacterial mutants in multi-drug resistant clinical UTI isolates leveraging a highly efficient CRISPR-Cas system; and 6) Provide consultations and support of study design for the Research Projects. Importantly, the MGBC will serve as a national resource for members of other George M. O'Brien Cooperative Research Centers and other NIDDK Urologic Research Programs. In close collaboration with the Administrative Core the MGBC will ensure adherence to all fiscal, administrative, and resource sharing policies. The MGBC will also contribute to the goals of the Educational Enrichment and Opportunity Pool Programs through lectures, student mentorship, and guidance on experimental design related to microbial genomics for Opportunity Pool awardees. Taken together, the Columbia O’Brien U54 MGBC will enable state- of-the-art characterization of microbes in UTIs, with a focus on evolving multi-drug resistance, by providing a complementary set of tools harnessing molecular biology, genetics, and ‘omics technologies.

微生物基因组学生物医学核心（MGBC）：项目摘要/摘要 哥伦比亚大学奥布莱恩泌尿外科研究中心的使命是确定遗传因素和 先天性畸形，例如囊泡分流，使人易于尿路 （UTI）并探索宿主 - 细菌代谢过程，例如血红素途径，以启用或阻止 感染。 支持Columnbia O'Brien泌尿外科研究中心微生物基因组学和生物医学的目标 核心（MGBC）将为生物群，扩展细菌培养，微生物基因组学提供高质量的服务 （微生物组分析，比较基因组学），微生物遗传学，研究设计和外展咨询 对于尿路感染的新型调查。 中央研究人员可以使用专门的基因组技术和协调的专家 为了改善基因组学和精确医学的严格性和可重复性。 MGBC在尿路感染中的抗菌素耐药性已建立了多药 抗性UTI隔离株。 哥伦比亚奥布莱恩中心的研究项目和正常的团结泳池获奖者，将提供给 更广泛的泌尿科研究界。 将多药耐药UTI的生物措施扩展到纽约市的多个地点，建立一个 来自临床明确定义明确的UTI症状的患者的尿液生物疗法，并进行尿液延长 生物识别培养 测序; 3）促进了主要的UTI致病克隆的基因组测序 UTI症状的决定因素； 4）在耐多药临床分离物中产生细菌突变体 利用高效的CRISPR-CAS系统； 6） 研究项目。 M. O'Brien合作研究中心和其他NIDDK泌尿科研究计划 使用行政核心，MGBC将确保遵守所有财政，非分别和资源共享 政策。 通过讲座，学生指导和与微生物有关的实验设计指导的计划 哥伦比亚大学U54 MGBC的基因组学综合池。 通过提供一个，utis中微生物的表征，重点是进化多药抗性。 编译组合的工具来利用分子生物学，遗传学和“ OMICS技术”。