Mouse Exercise and Metabolic Phenotyping Core

小鼠运动和代谢表型核心

基本信息

批准号：
10018900
负责人：
DAVID H WASSERMAN
金额：
$ 23.11万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-20 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10018900
关键词：
Acute Asses Biochemical Biological Assay Blood specimen Carbon Dioxide Chronic Data Doctor of Philosophy Energy Metabolism Enzyme-Linked Immunosorbent Assay Equipment Exercise Exercise Physiology Exercise Test Funding Glucagon Glycogen Hormones Individual Indwelling Catheter Infrastructure Insulin Laboratories Link Liver Measurement Measures Metabolic Modeling Mus Output Pathway Analysis Plasma Polyacrylamide Gel Electrophoresis Protocols documentation Publications Publishing Research Research Personnel Role Running Services Signal Pathway Signal Transduction Signaling Protein Skeletal Muscle Spectrophotometry Speed Testing Tissues Triglycerides United States National Institutes of Health Universities Western Blotting Work cost effective delta protein design energy balance exercise capacity exercise intensity exercise training experimental study ghrelin member metabolic phenotype metabolic profile mouse model programs respiratory response treadmill uptake

项目摘要

ABSTRACT The Mouse Exercise and Metabolic Phenotyping Core (Core B) will facilitate the design and execution of the exercise experiments for Program Project members. Core B leverages the facilities and infrastructure of an original NIH-funded Mouse Metabolic Phenotyping Center (MMPC, www.mmpc.org) at Vanderbilt University and the specific expertise of its Director, David Wasserman, PhD in exercise physiology. Core B has already designed and executed exercise studies that have been recently published by the Elmquist, Zigman and Williams laboratories, in addition to designing all of the exercise paradigms used to generate the respective preliminary data for the current application. (see publications section within the individual projects). Core B will provide the following Exercise Protocols and Measurements: Exercise Protocols  Incremental treadmill exercise test to assess peak O2 uptake and work capacity  Analysis of endurance capacity utilizing motorized treadmills  Perform acute high intensity interval exercise  Perform chronic exercise training Measurements  O2 uptake, CO2 output, and respiratory exchange ratio (RER) will be measured during or after exercise  Body mass and composition  Blood sampling through an indwelling catheter  Analysis of liver and skeletal muscle glycogen content  Analysis of biochemical signaling pathways  Analysis plasma analysis of insulin, ghrelin, glucagon, triglycerides (TG), and non-esterified fatty acids (NEFA)

抽象的小鼠运动和代谢表型核心（核心 B）将有助于设计和执行计划项目核心 B 的练习实验利用了一个设施和基础设施。范德比尔特大学原 NIH 资助的小鼠代谢表型中心 (MMPC, www.mmpc.org) 其主任、运动生理学博士 David Wasserman 已经具备了丰富的专业知识。设计并执行了 Elmquist、Zigman 和最近发表的运动研究威廉姆斯实验室，除了设计用于生成各自的练习范式之外当前应用程序的初步数据（请参阅各个项目中的出版物部分）。核心 B 将提供以下练习方案和测量：锻炼方案  增量跑步机运动测试，以评估峰值摄氧量和工作能力  利用电动跑步机分析耐力能力  进行急性高强度间歇运动  进行长期运动训练测量值  将在运动期间或运动后测量氧气吸收量、二氧化碳输出量和呼吸交换比 (RER)  体重和成分  通过留置导管进行血液采样肝脏和骨骼肌糖原含量分析  生化信号通路分析  胰岛素、生长素释放肽、胰高血糖素、甘油三酯 (TG) 和非酯化脂肪酸的血浆分析（国家能源局）