Mouse Exercise and Metabolic Phenotyping Core

小鼠运动和代谢表型核心

基本信息

批准号：
10677757
负责人：
DAVID H WASSERMAN
金额：
$ 22.2万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-20 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10677757
关键词：
Acute Biochemical Biological Assay Blood specimen Carbon Dioxide Chronic Data Doctor of Philosophy Energy Metabolism Enzyme-Linked Immunosorbent Assay Equipment Exercise Exercise Physiology Exercise Test Funding Glucagon Glycogen Hormones Individual Indwelling Catheter Infrastructure Insulin Laboratories Link Liver Measurement Measures Metabolic Modeling Motor Mus Nonesterified Fatty Acids Output Plasma Polyacrylamide Gel Electrophoresis Protocols documentation Publications Publishing Research Research Personnel Role Running Services Signal Pathway Signal Transduction Signaling Protein Skeletal Muscle Spectrophotometry Speed Testing Tissues Training Triglycerides United States National Institutes of Health Universities Western Blotting Work cost effective design energy balance exercise capacity exercise intensity exercise training experimental study ghrelin member metabolic phenotype mouse model programs respiratory response treadmill uptake

项目摘要

ABSTRACT The Mouse Exercise and Metabolic Phenotyping Core (Core B) will facilitate the design and execution of the exercise experiments for Program Project members. Core B leverages the facilities and infrastructure of an original NIH-funded Mouse Metabolic Phenotyping Center (MMPC, www.mmpc.org) at Vanderbilt University and the specific expertise of its Director, David Wasserman, PhD in exercise physiology. Core B has already designed and executed exercise studies that have been recently published by the Elmquist, Zigman and Williams laboratories, in addition to designing all of the exercise paradigms used to generate the respective preliminary data for the current application. (see publications section within the individual projects). Core B will provide the following Exercise Protocols and Measurements: Exercise Protocols  Incremental treadmill exercise test to assess peak O2 uptake and work capacity  Analysis of endurance capacity utilizing motorized treadmills  Perform acute high intensity interval exercise  Perform chronic exercise training Measurements  O2 uptake, CO2 output, and respiratory exchange ratio (RER) will be measured during or after exercise  Body mass and composition  Blood sampling through an indwelling catheter  Analysis of liver and skeletal muscle glycogen content  Analysis of biochemical signaling pathways  Analysis plasma analysis of insulin, ghrelin, glucagon, triglycerides (TG), and non-esterified fatty acids (NEFA)

抽象的鼠标运动和代谢表型核心（核心B）将有助于设计和执行计划成员的练习实验。范德比尔特大学的原始NIH资助的老鼠代谢表型中心（MMPC，www.mmpc.org）以及David Wasserman的特定专业知识，Birdialcise生理学。设计和执行的运动研究由Elmquist，Zigman和威廉姆斯实验室，除了设计用于产生各自的练习范式（请参阅各个项目中的出版物部分）。核心B将提供以下练习方案和测量：锻炼方案 增量跑步机运动测试以评估O2吸收峰值和工作能力 利用电动跑步机的耐力能力分析 进行急性高强度间隔运动 进行慢性运动训练测量 在运动期间或之后，将测量O2吸收，二氧化碳输出和呼吸道交换比（RER） 体重和成分 通过留置导管进行血液采样 分析肝脏和骨骼肌糖原含量 分析生化信号通路 分析胰岛素，甘以及甘油三酸酯，甘油三酸酯（TG）的血浆分析（NEFA）