Full Field OCT for Cellular Level Structural and Functional Retinal Imaging

用于细胞水平结构和功能性视网膜成像的全视场 OCT

• 批准号: 10052709
• 负责人: Nathan Doble
• 金额: $ 57.4万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10052709
• 关键词: Address; Age; Age related macular degeneration; Anesthetics; Animal Model; Animals; Austria; Back; Brain; Cells; Clinical; Collaborations; Cone; Dark Adaptation; Data; Development; Devices; Disease; Drusen; Electroretinography; Exhibits; Eye; Four-dimensional; Fundus photography; Future; Generations; Genes; Human; Image; Imaging technology; Individual; Kinetics; Lateral; Length; Light; Link; Maps; Measurement; Measures; Medical; Methods; Microscopic; Modality; Modeling; Mus; Neurons; Nonexudative age-related macular degeneration; Ohio; Optical Coherence Tomography; Optics; Outcome; Performance; Perimetry; Phase; Physiological; Physiology; Positioning Attribute; Protocols documentation; Publishing; Reporting; Reproducibility; Resolution; Retina; Retinal Cone; Retinal Degeneration; Retinal Diseases; Sampling; Scanning; Scheme; Signal Transduction; Source; Speed; Stimulus; Structure; Structure of retinal pigment epithelium; System; Techniques; Testing; Time; Transgenic Mice; Universities; Validation; Vertebrate Photoreceptors; Vision; Visual Fields; Visualization; Work; adaptive optics; analog; awake; base; clinical translation; cohort; comparative; computerized tools; cost; design; design and construction; digital; electric field; experimental study; human imaging; human model; human subject; imaging system; in vivo; in vivo imaging; instrumentation; light scattering; millisecond; mouse model; nanometer; normal aging; novel; novel therapeutics; response; retinal imaging; retinal rods; stem cell therapy; temporal measurement; tool

Project Summary The proposed design and construction of an optical coherence tomography (OCT) system, namely, a full-field (FF)-swept-source (SS)-OCT will allow rapid structural and functional measures of individual cone and rod photoreceptors (PRCs), the sub-retinal space (SRS) and retinal pigment epithelial (RPE) cells to an external light stimulus. Current OCT systems either have limited temporal resolution i.e. they are not fast enough to measure the neuronal response or lack the spatial resolution to resolve individual cells. Here, we will exploit the extremely high parallel image acquisition speed of FF-SS-OCT to study neuronal responses as short as a few milliseconds. Furthermore, as the system collects the whole back scattered electric field from the sample, numerical aberration correction (NAC) methods can be used allowing for the visualization of single cells without the need for techniques such as hardware based adaptive optics (AO). There are three stages to the proposed project: (i) the design and construction of FF-SS-OCT systems for both human and animal models (mice) of retinal disease - examining both species in parallel will speed up the clinical translation, (ii) testing the system performance in healthy retina of both humans and mice, (iii) measure the sensitivity of the system to detect microstructural functional changes by comparing age-matched controls to diseased cohorts. These will be early stage dry age-related macular degeneration (AMD) subjects and several AMD mouse models. Scientific rigor and reproducibility will be addressed by comparing FF-SS-OCT structural images to those obtained with our existing human and mice AO-OCT systems; functional measurements in human subjects will be compared to published data and also to clinical measures such as mfERG and visual fields. Functional measurements in mice will be compared to published data and also to the results from our first-generation mouse functional retinal imaging system and Ganzfeld ERG. Many potential therapies are under development for a range of ocular diseases, these systems fulfill a critical need for modalities that can not only determine whether the neurons are structurally intact but importantly are also exhibiting normal function.

项目摘要 拟议的设计和构建光学相干断层扫描（OCT）系统，即全场 （ff） - 铝制源（SS）-oct将允许快速的结构和功能衡量单个锥和杆 光感受器（PRC），视网膜下空间（SR）和视网膜色素上皮（RPE）细胞到外部光线 刺激。当前的OCT系统要么具有有限的时间分辨率，即它们的速度不足以测量 神经元反应或缺乏解决各个细胞的空间分辨率。在这里，我们将极端利用 FF-SS-OCT的高平行图像采集速度可在短达几毫秒内研究神经元反应。 此外，随着系统从样品中收集整个背部散射电场，数值畸变 可以使用校正（NAC）方法，允许可视化单个单元，而无需 基于硬件的自适应光学（AO）等技术。 拟议项目有三个阶段：（i）两者的FF-SS-OCT系统的设计和构建 视网膜疾病的人类和动物模型（小鼠） - 并行检查两个物种将加快临床 翻译，（ii）测试人类和小鼠健康视网膜中的系统性能，（iii）测量 通过比较年龄匹配的对照与检测微结构功能变化的敏感性 患病的队列。这些将是早期的干燥年龄相关黄斑变性（AMD）和几个 AMD鼠标模型。 科学的严谨性和可重复性将通过将FF-SS-OCT结构图像与那些图像进行比较来解决 使用我们现有的人类和小鼠AO-O-OCT系统获得；人类受试者的功能测量将 将其与已发布的数据以及MFERG和视野等临床指标进行比较。功能 将小鼠的测量与已发布的数据以及我们的第一代鼠标的结果进行比较 功能性视网膜成像系统和Ganzfeld ERG。 许多潜在的疗法正在为各种眼部疾病开发，这些系统实现了关键 需求不仅可以确定神经元是否在结构上是完整的，而且重要的是 也表现出正常功能。