In-vivo, High Contrast Imaging of the Human Rod Photoreceptor Mosaic

人杆光感受器马赛克的体内高对比度成像

• 批准号: 8326718
• 负责人: Nathan Doble
• 金额: $ 30万
• 依托单位: NEW ENGLAND COLLEGE OF OPTOMETRY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8326718
• 关键词: Affect; Age related macular degeneration; Animal Model; Blindness; Cell Density; Cells; Clinical; Clinical Trials; Complement; Dark Adaptation; Data; Development; Disease; Eye; Fundus; Goals; Human; Image; Imagery; Imaging technology; Individual; Lead; Leukocytes; Life; Masks; Measures; New England; Optics; Optometry; Outcome Measure; Patients; Performance; Pharmaceutical Preparations; Pharmacotherapy; Process; Property; Reporting; Resolution; Retina; Retinal; Retinal Cone; Retinal Diseases; Retinitis Pigmentosa; Signal Transduction; Simulate; Structure; Structure of retinal pigment epithelium; System; Techniques; Testing; Time; Work; adaptive optics; arm; cell type; college; density; disease diagnosis; improved; in vivo; optical imaging; receptor; receptor coupling; retinal rods; success; tool

Project Summary The goal is to enhance the resolution and contrast of in-vivo images of the rod photoreceptor mosaic taken with high resolution adaptive optics (AO) equipped retinal cameras. There has been great success in imaging other cell types such as the cones, retinal pigment epithelium and leukocytes cells but to date nobody has reported in-vivo evidence for the rods. The project will examine advanced optical techniques that can be combined with AO to further improve the contrast of the retinal images at the spatial scale of the rods and foveal cones. These may be advanced masks to suppress unwanted structure in the images complemented with approaches such as using the Stiles- Crawford effect and deconvolution. Achieving this goal will allow one, for example, to count individual rod cells and calculate cell densities which can then be compared to histological measures. Initially, young, normal subjects will be imaged to refine and optimize the new imaging approaches. As the work progresses, the study will examine diseases that target the rod cells such as retinitis pigmentosa and age related macular degeneration and relate the changes observed to standard clinical tests. This work will lead to better understanding of retinal structure in both normal and diseased eyes. With new drug treatments being developed in animal models, the direct non-invasive imaging of rod cells would provide an important outcome measure when these therapies are transitioned into human clinical trials.

项目摘要 目的是增强杆光感受器镶嵌的体内图像的分辨率和对比度 具有高分辨率自适应光学元件（AO）具有视网膜相机。成像取得了巨大成功 其他细胞类型，例如锥体，视网膜色素上皮和白细胞细胞，但迄今为止没有人 报道了杆的体内证据。 该项目将检查可以与AO结合的高级光学技术，以进一步改善 视网膜图像在杆和凹锥的空间尺度上的对比。这些可能是高级面具 在图像中抑制不需要的结构，该结构与诸如使用stiles的方法相辅相成。 克劳福德效应和反卷积。实现此目标将允许一个人计算单个杆单元 并计算细胞密度，然后将其与组织学测量进行比较。 最初，将成像年轻的正常受试者以完善和优化新的成像方法。作为 工作进展，该研究将检查针对棒细胞（例如视网膜炎）和年龄的疾病 相关的黄斑变性并将观察到的变化与标准临床测试有关。 这项工作将在正常眼和患病的眼睛中更好地理解视网膜结构。有了新 在动物模型中开发的药物处理，杆细胞的直接非侵入性成像将提供 当这些疗法转移到人类临床试验中时，这是一个重要的结果指标。