Genotyping/Virus Resource Core

基因分型/病毒资源核心

基本信息

批准号：
10013207
负责人：
DAVID Paul ZENISEK
金额：
$ 20.26万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2022-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10013207
关键词：
Biological Cell Differentiation process Communities Consultations Core Facility Core Grant Custom Dependovirus Equipment Gene Delivery Genetic Techniques Genetically Modified Animals Genotype Goals Human Resources Infrastructure Laboratories Laboratory Personnel Maintenance Mediating Modernization Molecular Molecular Biology Molecular Biology Techniques Molecular Genetics Mus Neurons Pathogenicity Persons Plasmids Postdoctoral Fellow Preparation Procedures Production Research Research Personnel Resources Savings Services Students Techniques Time Training Training Support Training and Infrastructure Transgenic Animals Universities Viral Viral Vector Virus Vision Vision research cost design experimental study genetic manipulation immunogenicity innovation laboratory experience molecular array novel virus programs tool transgene delivery transgene expression vector virus resource

项目摘要

Genotyping and Virus Production Core Module Abstract The Genotyping and Virus Production Core Module will provide the means to incorporate modern molecular and genetic techniques into the research programs of the entire vision research community at Yale. First, the Module will supply genotyping and virus preparation assistance and services for laboratories working with transgenic animals and/or viral techniques. Second, it provides and maintains a range of molecular biology equipment to be shared among vision researchers and aids investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques. Third, it trains new investigators and students in basic molecular biology techniques, including the use of viral vectors. Viral vectors are powerful tools for gene delivery in terminally differentiated cells. For example, adeno-associated viruses (AAVs) have enjoyed increasingly wide application in vision research for their many favorable features that include the lack of pathogenicity, low immunogenicity, and the ability to maintain long-term transgene expression in neurons. The Genotyping and Virus Production core module will be sufficient and well equipped to provide both virus vector design and virus production to meet a variety of research needs among vision researchers at Yale. Although many of the common viruses can be purchased from viral core facilities outside of Yale, having an in- house capability for virus design and production will significantly enhance vision research by saving time and cost and facilitating more efficient and innovative virus design with the convenience of in-person consultation for specific research needs.

基因分型和病毒生产核心模块摘要基因分型和病毒生产核心模块将提供合并现代分子的手段以及耶鲁大学整个视觉研究界的研究计划的遗传技术。首先，模块将为与之合作的实验室提供基因分型和病毒准备帮助和服务转基因动物和/或病毒技术。其次，它提供并维护一系列分子生物学通过为人们提供服务，可以在视觉研究人员和AIDS调查人员中共享的设备没有基本或晚期分子生物学技术的设施。第三，它训练新的调查员和基本分子生物学技术的学生，包括使用病毒载体。病毒向量很强大终末分化细胞中基因递送的工具。例如，腺相关病毒（AAV）具有在视觉研究中享受越来越广泛的应用程序，包括他们的许多有利功能，包括缺乏致病性，低免疫原性以及维持神经元中长期转基因表达的能力。基因分型和病毒生产核心模块将足够且配备良好，可以提供病毒媒介设计和病毒生产以满足耶鲁大学视力研究人员的各种研究需求。尽管可以从耶鲁以外的病毒核心设施中购买许多常见病毒，但有一个内部的病毒病毒设计和生产的房屋能力将通过节省时间和成本和促进更高效，更具创新的病毒设计，以方便面对面咨询满足特定的研究需求。