MOLECULAR BIOLOGY MODULE

分子生物学模块

• 批准号: 7286514
• 负责人: DAVID Paul ZENISEK
• 金额: $ 1.31万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7286514
• 关键词: Biological; Coca; Computational Technique; Core Facility; Electroporation; Equipment; Future; Genotype; Immunohistochemistry; Laboratories; Maintenance; Molecular; Molecular Biology; Molecular Biology Techniques; Ophthalmology; Physiology; Plasmids; Polymerase Chain Reaction; Purpose; Range; Research; Research Personnel; Resources; Reverse Transcriptase Polymerase Chain Reaction; Robotics; Running; Services; Students; System; Techniques; Transfection; Transgenic Animals; Universities; Viral; Vision; Vision research; Western Blotting; Work; design; experience; post-doctoral training; pressure

The purpose of the molecular biology core is i) to provide and maintain a wide range of equipment to be shared among vision researchers; 2) aid investigators by providing a service for those without the facilities for basic or advanced molecular biology techniques; 3) help new investigators and students acquire basic molecular biological techniques; 4) provide genotyping services for laboratories working with transgenic animals. The resources provided by this module will be applied toward maintenance of existing equipment for shared usage among vision researchers throughout the university, to employ two technical staff and to purchase a quantitative RT-PCR system to relieve pressure on existing machines due to heavy usage. One technician, Ms. Adrienne LaRue Marabelle, will have bench space in Brady Memorial Laboratory (BML) 224 and will handle the genotyping of transgenic animals and will oversee the use of the robotic workstation and microarray core facility. Ms. Marabelle has over 15 years of experience in molecular biology techniques and has been running the microarray facility in the Department of Ophthalmology for the past 10 years. A second technician, Mr. Steven Viviano, will have bench space in SHM-B1O3 and will work with and train post-doctoral fellows and graduate students in various molecular techniques including Western blotting, quantitative PCR, immunohistochemistry. He will also design and prepare plasmids for electroporation or viral transfection for vision research investigators that are not equipped to do so in their own laboratories. The work of Mr. Viviano will be carried out using equipment in the laboratory of Dr. Zenisek and core facilities within the Department of Physiology and will be closely supervised by Dr. Zenisek. Several vision laboratories have primary expertise in electrophysiological, anatomical and computational techniques and core support has been instrumental in enabling these vision researchers to increasingly employ molecular biology techniques in their research. This module has enjoyed considerable usage in the past and will continue to do so in the future. It is anticipated that Drs. Crair, McCormick, Tian and Zenisek will make use of the genotyping service, Drs. Hoh, Rizzolo and Zeiss will make use of the microarray facility, Drs. Coca- Prados, Crair, Hoh, Rizzolo, Tian and Zenisek will make use of core facilities in the Brady Memorial Laboratory building and Drs. Coca-Prados, Crair, Hoh, Rizzolo, Tian, Zeiss and Zenisek will make use of Mr. Viviano's services.

分子生物学核心的目的是i）提供和维护多种设备以共享 在视力研究人员中； 2）援助调查人员为没有基本设施的人提供服务或 晚期分子生物学技术； 3）帮助新的研究人员和学生获得基本分子 生物技术； 4）为与转基因动物一起工作的实验室提供基因分型服务。 该模块提供的资源将用于维护现有设备以共享 整个大学的视觉研究人员中使用，雇用两名技术人员并购买 定量RT-PCR系统，以减轻由于大量使用而对现有机器的压力。一位技术员，女士。 Adrienne Larue Marabelle，将在Brady Memorial Laboratory（BML）224设有长凳空间 转基因动物的基因分型，并将监督机器人工作站和微阵列核心的使用 设施。 Marabelle女士在分子生物学技术方面拥有超过15年的经验，并且一直在运行 过去10年来，眼科系的微阵列设施。第二位技术人员，先生 史蒂文·维维亚诺（Steven Viviano），将在SHM-B1O3中设有台式空间，并将与博士后研究员一起训练和培训 各种分子技术的研究生，包括蛋白质印迹，定量PCR， 免疫组织化学。他还将设计和准备用于电穿孔或病毒转染的质粒 视觉研究研究人员没有能力在自己的实验室中这样做。维维亚诺先生的工作 将在Zenisek博士和部门内的核心设施的实验室中使用设备进行 生理学，并将由Zenisek博士密切监督。 几个视力实验室在电生理，解剖学和计算方面具有主要专业知识 技术和核心支持一直在使这些远见研究人员越来越多地利用 他们的研究中的分子生物学技术。该模块过去享有相当大的用法 将来会继续这样做。预计博士。 Crair，McCormick，Tian和Zenisek将制作 基因分型服务的使用，博士。 Hoh，Rizzolo和Zeiss将使用Microaray设施，Drs。可口可乐 Prados，Crair，Hoh，Rizzolo，Tian和Zenisek将利用Brady纪念实验室中的核心设施 建筑和博士。可口可乐，Crair，Hoh，Rizzolo，Tian，Zeiss和Zenisek将利用Viviano先生的 服务。