Role of miRNAs on the regulation of juvenile hormone synthesis in mosquitoes

miRNA对蚊子保幼激素合成调节的作用

• 批准号: 10038172
• 负责人: Marcela Nouzova
• 金额: $ 22.13万
• 依托单位: FLORIDA INTERNATIONAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10038172
• 关键词: Adult; Aedes; Animals; Area; Asses; Basic Science; Binding; Biogenesis; Biology; Blood; Chemicals; Chikungunya fever; Coenzyme A; Corpora Allata; Culicidae; Dengue; Dengue Fever; Development; Digestion; Enzymes; Event; Excretory function; Experimental Models; Female; Gene Expression; Gene Expression Regulation; Genes; Genetic Transcription; Goals; High-Throughput Nucleotide Sequencing; Hormonal; Immunoprecipitation; In Vitro; Incidence; Insect Control; Insect Viruses; Insecta; Insecticides; Intake; Juvenile Hormones; Knowledge; Lead; Libraries; Link; Liquid substance; Malaria; Mass Spectrum Analysis; Messenger RNA; MicroRNAs; Molting; Monitor; Mosquito Control; Mosquito-borne infectious disease; Nutrient; Nutritional status; Organism; Ovary; Pathway interactions; Physiological; Plants; Play; Proteins; Public Health; Pupa; Quantitative Reverse Transcriptase PCR; Regulation; Regulator Genes; Reproduction; Reproductive Process; Research; Resistance; Role; Sampling; Signal Transduction; Small RNA; Testing; Tissues; Transcript; Untranslated RNA; ZIKA; absorption; base; chikungunya; design; differential expression; egg; experience; experimental study; hormonal signals; hormone biosynthesis; in vivo; inhibitor/antagonist; novel strategies; public health relevance; success; sugar; transcriptome; transcriptome sequencing; transcriptomics; vector control; zika fever

PROJECT SUMMARY Recent dramatic increases in the incidence of mosquito-borne diseases, like Malaria, Zika, Chikungunya and Dengue Fever, and the wide-spread resistance of mosquitoes to insecticides emphasizes the need for new approaches for insect control based on mosquito-specific agents. The discovery of such mosquito-specific control agents depends on continued basic research on the biology of mosquitoes. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression in organisms as diverse as plants, animals, insects and viruses. Sequence specific binding of miRNAs to complementary regions on mRNAs can either down-regulate or up- regulate expression of the encoded protein. A growing body of evidence indicates that miRNAs contribute to the control of tissue- and stage-specific gene expression in mosquitoes; playing key roles modulating sugar absorption, fluid excretion, blood intake and digestion, egg maturation and survival in Ae. aegypti. On the other hand, little is known about the role of miRNAs on the regulation of juvenile hormone (JH) biosynthesis in insects. In the female mosquito, JH signals the completion of the ecdysis to the adult stage, and initiates reproductive processes. JH titer is essentially determined by the rate at which the corpora allata (CA) synthesizes JH. High- throughput small RNA sequencing of CA showed dramatic alterations of miRNA profiles among the CA of pupa (low JH synthesis), sugar-fed (high JH synthesis) and blood fed (low JH synthesis) adult females. We now intend to further study the role of CA-specific and differentially expressed miRNAs in the regulation of JH biogenesis. Completing the proposed research could lead to the identification of targets for designing new, specific and affordable strategies suitable for mosquito control.

项目摘要 最近的蚊子传播疾病发生率的巨大增加，如疟疾，Zika， 基孔肯雅和登革热，以及蚊子对杀虫剂的广泛抵抗力 强调了基于蚊子特异性药物基于昆虫控制的新方法的需求。 这种蚊子特异性控制剂的发现取决于对 蚊子的生物学。 microRNA（miRNA）是调节基因的小非编码RNA 像植物，动物，昆虫和病毒一样多样化的生物体中的表达。特定于序列 miRNA与互补区域对mRNA的结合可以下调或上调 调节编码蛋白的表达。越来越多的证据表明miRNA 有助于控制蚊子中组织和阶段特异性基因表达；播放钥匙 调节糖吸收，液体排泄，血液摄入和消化，卵成熟的角色 和AE的生存。埃及。另一方面，关于miRNA在 昆虫中青少年激素（JH）生物合成的调节。在女性蚊子中，JH信号 逐步完成成人阶段，并启动生殖过程。 JH滴度是 基本上取决于Corpora allata（CA）合成JH的速率。高的- Ca的吞吐量小RNA测序显示miRNA曲线的急剧变化 PUPA（低JH合成），糖喂养（高JH合成）和血液（低JH）的CA（低JH） 合成）成年女性。现在，我们打算进一步研究CA特异性和差异性的作用 在JH生物发生的调节中表达了miRNA。完成拟议的研究可能 导致确定设计新的，特定和负担得起的策略的目标 用于蚊子控制。