Role of miRNAs on the regulation of juvenile hormone synthesis in mosquitoes

miRNA对蚊子保幼激素合成调节的作用

基本信息

批准号：
10176404
负责人：
Marcela Nouzova
金额：
$ 14.75万
依托单位：
FLORIDA INTERNATIONAL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-01 至 2023-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10176404
关键词：
Adult Aedes Animals Area Basic Science Binding Biogenesis Biology Blood Chemicals Chikungunya fever Coenzyme A Corpora Allata Culicidae Dengue Dengue Fever Development Digestion Enzymes Event Excretory function Experimental Models Female Gene Expression Gene Expression Regulation Genes Genetic Transcription Goals High-Throughput Nucleotide Sequencing Hormonal Immunoprecipitation In Vitro Incidence Insect Control Insect Viruses Insecta Insecticides Intake Juvenile Hormones Knowledge Lead Libraries Link Liquid substance Malaria Mass Spectrum Analysis Messenger RNA MicroRNAs Molting Monitor Mosquito Control Mosquito-borne infectious disease Nutrient Nutritional status Organism Ovary Pathway interactions Physiological Plants Play Proteins Public Health Pupa Quantitative Reverse Transcriptase PCR Regulation Regulator Genes Reproduction Reproductive Process Research Resistance Role Sampling Signal Transduction Small RNA Testing Tissues Transcript Untranslated RNA ZIKA absorption base chikungunya design differential expression egg experience experimental study hormonal signals hormone biosynthesis in vivo inhibitor/antagonist novel strategies public health relevance success sugar transcriptome transcriptome sequencing transcriptomics vector control zika fever

项目摘要

PROJECT SUMMARY Recent dramatic increases in the incidence of mosquito-borne diseases, like Malaria, Zika, Chikungunya and Dengue Fever, and the wide-spread resistance of mosquitoes to insecticides emphasizes the need for new approaches for insect control based on mosquito-specific agents. The discovery of such mosquito-specific control agents depends on continued basic research on the biology of mosquitoes. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression in organisms as diverse as plants, animals, insects and viruses. Sequence specific binding of miRNAs to complementary regions on mRNAs can either down-regulate or up- regulate expression of the encoded protein. A growing body of evidence indicates that miRNAs contribute to the control of tissue- and stage-specific gene expression in mosquitoes; playing key roles modulating sugar absorption, fluid excretion, blood intake and digestion, egg maturation and survival in Ae. aegypti. On the other hand, little is known about the role of miRNAs on the regulation of juvenile hormone (JH) biosynthesis in insects. In the female mosquito, JH signals the completion of the ecdysis to the adult stage, and initiates reproductive processes. JH titer is essentially determined by the rate at which the corpora allata (CA) synthesizes JH. High- throughput small RNA sequencing of CA showed dramatic alterations of miRNA profiles among the CA of pupa (low JH synthesis), sugar-fed (high JH synthesis) and blood fed (low JH synthesis) adult females. We now intend to further study the role of CA-specific and differentially expressed miRNAs in the regulation of JH biogenesis. Completing the proposed research could lead to the identification of targets for designing new, specific and affordable strategies suitable for mosquito control.

项目概要最近蚊媒疾病的发病率急剧增加，例如疟疾、寨卡病毒、基孔肯雅热和登革热，以及蚊子对杀虫剂的广泛耐药性强调需要基于蚊子特异性药剂的昆虫控制新方法。这种蚊子特异性控制剂的发现取决于持续的基础研究蚊子的生物学。 MicroRNA (miRNA) 是调节基因的小型非编码 RNA 在植物、动物、昆虫和病毒等多种生物体中表达。序列特异性 miRNA 与 mRNA 互补区域的结合可以下调或上调调节编码蛋白的表达。越来越多的证据表明 miRNA 有助于控制蚊子的组织和阶段特异性基因表达；弹奏琴键调节糖吸收、液体排泄、血液摄入和消化、卵子成熟的作用和 Ae 中的生存。埃及伊蚊。另一方面，人们对 miRNA 在细胞中的作用知之甚少。昆虫保幼激素（JH）生物合成的调节。在雌性蚊子中，JH 发出信号完成蜕皮到成虫阶段，并开始生殖过程。 JH效价为本质上是由 corpora allata (CA) 合成 JH 的速率决定的。高的- CA 的吞吐量小 RNA 测序显示 miRNA 谱的显着改变蛹（低 JH 合成）、糖饲喂（高 JH 合成）和血饲（低 JH 合成）的 CA 合成）成年女性。我们现在打算进一步研究 CA 特异性和差异化的作用表达 miRNA 在 JH 生物发生的调节中。完成拟议的研究可以导致确定目标，以设计新的、具体的和负担得起的合适战略用于控制蚊子。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Juvenile hormone mediates lipid storage in the oocytes of Dipetalogaster maxima.

DOI：
10.1016/j.ibmb.2020.103499
发表时间：
2020-11
期刊：
Insect biochemistry and molecular biology
影响因子：
3.8
作者：
F. O. Ramos;J. Leyria;M. Nouzová;Leonardo L. Fruttero;F. G. Noriega;L. Canavoso
通讯作者：
F. O. Ramos;J. Leyria;M. Nouzová;Leonardo L. Fruttero;F. G. Noriega;L. Canavoso

Approaches and Tools to Study the Roles of Juvenile Hormones in Controlling Insect Biology.

DOI：
10.3390/insects11120858
发表时间：
2020-12-03
期刊：
Insects
影响因子：
3
作者：
Noriega FG;Nouzova M
通讯作者：
Nouzova M

Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.

DOI：
10.1038/s41598-022-25009-4
发表时间：
2022-11-28
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Nouzova, Marcela;Edwards, Marten J.;DeGennaro, Matthew;Leyva, Dennys;Tose, Lilian V.;Fernandez-Lima, Francisco;Noriega, Fernando G.
通讯作者：
Noriega, Fernando G.

Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage.

DOI：
10.1038/s41598-021-89025-6
发表时间：
2021-05-05
期刊：
Scientific reports
影响因子：
4.6
作者：
Tose LV;Weisbrod CR;Michalkova V;Nouzova M;Noriega FG;Fernandez-Lima F
通讯作者：
Fernandez-Lima F

Role of Methoprene-tolerant in the regulation of oogenesis in Dipetalogaster maxima.

DOI：
10.1038/s41598-022-18384-5
发表时间：
2022-08-20
期刊：
SCIENTIFIC REPORTS
影响因子：
4.6
作者：
Ramos, Fabian O.;Nouzova, Marcela;Fruttero, Leonardo L.;Leyria, Jimena;Ligabue-Braun, Rodrigo;Noriega, Fernando G.;Canavoso, Lilian E.
通讯作者：
Canavoso, Lilian E.