Ohio State University Neuroscience Center Core-Core E

俄亥俄州立大学神经科学中心核心-核心 E

• 批准号: 10005513
• 负责人: Anthony Brown
• 金额: $ 11.68万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10005513
• 关键词: 3-Dimensional; Applications Grants; Cells; Color; Communities; Complex; Computer software; Consultations; Data; Data Set; Educational Status; Embryo; Ensure; Environment; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorochrome; Funding; Image; Image Analysis; Journals; Laboratories; Light; Microscope; National Institute of Neurological Disorders and Stroke; Neurons; Neurosciences; Ohio; Paper; Productivity; Progress Reports; Proteins; PubMed; Publications; Publishing; Research; Research Personnel; Research Project Grants; Research Support; Running; S10 grant; Sampling; Scanning; Schedule; Service delivery model; Services; Slice; Specimen; Stream; System; Time; Tissues; Training; United States National Institutes of Health; Universities; Wait Time; Work; Zebrafish; confocal imaging; cost; design; experience; experimental study; flexibility; image processing; imaging facilities; instrument; instrumentation; large datasets; microscopic imaging; photoactivation; ranpirnase; web site

CORE E (IMAGING) ABSTRACT The Imaging Core provides access to well-maintained point-scanning and spinning disk confocal microscopes as well as expert consultation, training and assistance with confocal fluorescence microscopy of cells, tissue slices and zebrafish embryos, both in the living and fixed state. Advanced capabilities include long-term 4D multi-color time-lapse imaging, simultaneous dual-color imaging, fluorescence photoactivation and FRAP, FRET, image stitching and 3D-rendering. The Core can perform imaging sessions with investigators, if necessary, and can provide training and advice on image processing and analysis.

核心 E（成像）摘要 成像核心提供维护良好的点扫描和转盘共焦显微镜 以及细胞、组织共聚焦荧光显微镜方面的专家咨询、培训和帮助 切片和斑马鱼胚胎，包括活体和固定状态。高级功能包括长期 4D 多色延时成像、同步双色成像、荧光光激活和 FRAP， FRET、图像拼接和 3D 渲染。核心可以与研究人员进行成像会议，如果 必要时，可以提供图像处理和分析方面的培训和建议。