Towards noninvasive diagnosis of malaria

迈向疟疾的无创诊断

基本信息

批准号：
10005582
负责人：
Audrey Ragan Odom John
金额：
$ 18.82万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-01-18 至 2021-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10005582
关键词：
Africa Aftercare Antimalarials Area Asia Australia Biological Markers Blood Circulation Blood specimen Breath Tests Centers for Disease Control and Prevention (U.S.)Cessation of life Characteristics Child Childhood Clinical Clinical Research Culicidae Data Detection Development Devices Diagnosis Diagnostic Diagnostic tests Enrollment Ensure Exhalation Falciparum Malaria Gene Deletion Global Change Goals HRP-2 protein Health Campaign Infection Interdisciplinary Study Investigation Investments Kenya Laboratory Research Lead Location Machine Learning Malaria Malawi Mass Spectrum Analysis Medical Research Molecular Nose Parasitemia Parasites Parasitology Persons Pilot Projects Plasmodium falciparum Public Health Reporting Reproducibility Research Research Institute Risk Techniques Technology Testing Translating base biomarker discovery breath composition candidate marker clinical Diagnosis cohort cost density diagnostic biomarker experience global health improved innovation malaria infection noninvasive diagnosis pediatric patients portability response sensor specific biomarkers success tool transmission process treatment response volatile organic compound

项目摘要

PROJECT SUMMARY Plasmodium falciparum causes the deadliest form of malaria, which is responsible for nearly one million deaths annually. There is currently no perfect test to detect if a person has malaria. This is because all available tests have challenges and are all based on testing a blood sample. We have identified volatile organic compounds that are produced by the malaria parasite in culture. In a recent pilot study that was conducted in Lilongwe, Ma- lawi, we found that similar compounds are also present in the breath of children who have uncomplicated falci- parum malaria. We also observed a global change in the breath profile of children with malaria. The objectives of this proposal are to identify candidate biomarkers of malaria, with the long-term goal to develop a new non- invasive test to diagnose clinical malaria infection. Supported by our strong preliminary data that indicate that this strategy will be successful, our objectives will be met through two specific aims: 1) determine reproducible changes in the breath composition in response to malaria infection, in an independent location. 2) Relate breath changes with parasite development stages. Our approach is innovative, since we will enroll naturally infected children in the field. Children harbor the highest parasite and gametocyte densities. The proposed re- search is significant, because we will progress in development of new, much-needed malaria diagnostic tool that will not rely on blood samples.

项目摘要恶性疟原虫引起最致命的疟疾形式，疟疾造成近100万人死亡每年。目前没有完美的测试来检测一个人是否患有疟疾。这是因为所有可用的测试面临挑战，全部基于测试血液样本。我们已经确定了挥发性有机化合物疟疾在培养物中产生的。在最近在Lilongwe进行的一项试点研究中 Lawi，我们发现在不复杂的儿童的呼吸中也存在类似的化合物。疟疾疟疾。我们还观察到疟疾儿童的呼吸形象发生了全球变化。目标该提议的内容是确定疟疾的候选生物标志物，其长期目标是开发新的非 - 侵入性测试以诊断临床疟疾感染。在我们强大的初步数据的支持下，表明该策略将成功，我们的目标将通过两个具体目标实现：1）确定可重复性响应疟疾感染的呼吸组成的变化，在独立的位置。 2）相关呼吸随寄生虫发育阶段而变化。我们的方法是创新的，因为我们会自然而然地注册感染了该领域的儿童。儿童拥有最高的寄生虫和配子细胞密度。拟议的重新搜索很重要，因为我们将在开发新的，急需的疟疾诊断工具方面发展这不会依靠血液样本。

项目成果

期刊论文数量（20）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Concordance of Upper and Lower Respiratory Tract Samples for SARS-CoV-2 in Pediatric Patients: Research Letter.

DOI：
10.1097/aln.0000000000003765
发表时间：
2021-06-01
期刊：
Anesthesiology
影响因子：
8.8
作者：
Lin EE;Akaho EH;Sobilo A;Blatz AM;Otto WR;Odom John AR
通讯作者：
Odom John AR

Volatile profiling distinguishes Streptococcus pyogenes from other respiratory streptococcal species.

DOI：
10.1128/msphere.00194-23
发表时间：
2023-10-24
期刊：
MSPHERE
影响因子：
4.8
作者：
Berna, Amalia Z.;Merriman, Joseph A.;Mellett, Leah;Parchment, Danealle K.;Caparon, Michael G.;Odom John, Audrey R.
通讯作者：
Odom John, Audrey R.

Haloacid Dehalogenase Proteins: Novel Mediators of Metabolic Plasticity in Plasmodium falciparum.