High-definition, wide field of view corneal imaging

高清、宽视场角膜成像

• 批准号: 10007064
• 负责人: Cristina Canavesi
• 金额: $ 79.57万
• 依托单位: LIGHTOPTECH CORPORATION
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10007064
• 关键词: 3-Dimensional; Address; Area; Assessment tool; Blindness; Cell Count; Cell Density; Cell Survival; Cell Viability Process; Cellular Morphology; Characteristics; Clinic; Confocal Microscopy; Cornea; Corneal Diseases; Disease; Endothelial Cells; Evaluation; Eye; Eye Banks; Goals; Gold; Grant; Health; Human; Image; Imaging Device; Imaging Techniques; Industry; Infection; Innovation Corps; International; Lead; Legal patent; Machine Learning; Measurement; Measures; Methods; Microscope; Microscopy; Monitor; Morphology; Operative Surgical Procedures; Ophthalmology; Optics; Organ Transplantation; Patient imaging; Phase; Positioning Attribute; Process; Research; Resolution; Rights; Sampling; Sampling Errors; Small Business Innovation Research Grant; Standardization; Structure; Technology; Thick; Thinness; Time; Tissue Donors; Tissue Transplantation; Tissues; Training; Transplantation; United States National Institutes of Health; Universities; Variant; Visual; Visual impairment; Visual system structure; Visualization software; base; clinical development; clinical imaging; commercial application; density; image processing; improved; in vivo; in vivo regeneration; innovation; instrument; instrumentation; microscopic imaging; multidisciplinary; neurosensory; novel; phase 2 study; programs; prototype; screening; three-dimensional visualization; tool; trend

The cornea is the primary focusing structure of our visual system. Infections and diseases in the tissue can impair vision and lead to blindness, even in eyes with intact neurosensory function. Corneal disease is one of the leading causes of visual deficiency and blindness in the world. Tissue evaluation is an important step for assessing the health of the donor cornea and its appropriateness for different types of placement, yet this process suffers from high subjectivity. High-definition corneal imaging is needed to assist in selection of the most appropriate tissue for transplant. Progress on this front would greatly serve public need, as the cornea is the most commonly transplanted tissue worldwide, with nearly 185,000 transplants annually. Thus, a more sensitive and quantitative method for objective evaluation of tissue at eye banks is needed. We have developed a 3D high-definition imaging instrument based on Gabor-Domain Optical Coherence Microscopy (GDOCM). Our SBIR Phase I research successfully accomplished all Aims and demonstrated the feasibility of quantitative assessment of corneal tissue over a large field of view with GDOCM. Our Phase I results demonstrated that GDOCM has the following key advantages over existing corneal imaging techniques, which include specular and confocal microscopy: 1) improved accuracy of tissue qualification with 4-10x increase in field of view that reduces sampling error – this will provides a truer assessment of the overall tissue characteristics; 2) ability to simultaneously measure corneal thickness, quantify endothelial cell density, and identify morphological variations due to corneal disease – this will lead to complete corneal evaluation in a single instrument; 3) leveraging machine learning innovations to minimize variability induced by users – this will result in a more objective evaluation; 4) enhanced 3D cellular-level imaging of thin translucent endothelial cells – this will enable a detailed understanding of cell viability. The results of the proposed Phase studies II will demonstrate that GDOCM can provide high-definition, 3D visualization of corneal structures with immediate commercial application for qualification of donor tissue in eye banks, and with a path to in vivo clinical imaging of patients with corneal disease.

角膜是我们视觉系统的主要聚焦结构。组织中的感染和疾病可以 即使在具有完整的神经感觉功能的眼睛中，也会损害视力并导致失明。角膜疾病是 世界上视觉缺乏和失明的主要原因。组织评估是重要的一步 评估捐赠者角膜的健康及其对不同类型的安置的适当性，但这是 过程遭受高主观性。需要高清角膜成像以帮助选择 最合适的组织用于移植。在这方面的进步将极大地满足公众需求，因为角膜是 全世界最常见的组织最常移植组织，每年将接近185,000个移植。那，更多 需要对眼库的组织客观评估的敏感和定量方法。 我们已经开发了一种基于Gabor域光学连贯性的3D高清成像仪器 显微镜（GDOCM）。我们的SBIR阶段研究成功完成了所有目标，并证明了 用GDOCM在大型视野上对角膜组织进行定量评估的可行性。我们的阶段结果 证明GDOCM比现有角膜成像技术具有以下关键优势， 包括特定和共聚焦显微镜：1）提高组织资格的准确性，增加了4-10倍 减少采样误差的视野 - 这将提供对整个组织的真实评估 特征; 2）能够同时测量角膜厚度，量化内皮细胞密度和 确定由于角膜疾病引起的形态变化 - 这将导致单一的角膜评估 乐器; 3）利用机器学习创新以最大程度地减少用户引起的可变性 - 这将导致 在更客观的评估中； 4）增强了薄半透明内皮细胞的3D细胞水平成像 - 这 将对细胞活力有详细的了解。 提出的阶段研究结果II将证明GDOCM可以提供高清，3D 具有立即商业应用的角膜结构的可视化供体组织在眼睛中的资格 银行，并具有角膜疾病患者体内临床成像的途径。