Core C: Genomics & Bioinformatics Core

核心 C：基因组学

• 批准号: 10006528
• 负责人: Olivier Elemento
• 金额: $ 38.15万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-18 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10006528
• 关键词: ATAC-seq; Bioinformatics; Biological Assay; Cell Line; Cells; ChIP-seq; Clinical Research; DNA; DNA sequencing; Equipment; Genomics; Institutes; Laboratories; Mantle Cell Lymphoma; Monitor; Plasma; Publications; Research Project Grants; Sampling; Services; Techniques; base; clinical sequencing; data sharing; epigenomics; exome sequencing; precision medicine; single-cell RNA sequencing; transcriptome sequencing

ABSTRACT The genomics and bioinformatics core (GBC) will provide the projects with centralized facilities, equipment and expertise for whole-exome sequencing, transcriptome sequencing, single cell RNAseq, plasma DNA sequencing, ATAC-seq and ChIP-seq. All research projects will use the GBC. The GBC will be led by two co- PIs with complementary expertise, Dr Elemento and Dr Mason. The GBC will provide four main types of services: 1) integrated whole-exome sequencing and transcriptome sequencing of clinical samples and cell lines, 2) integrated epigenomic profiling of clinical and research samples using ERRBS, ChIPseq and ATAC- seq, 3) single cell profiling and plasma DNA monitoring and 4) to perform integrative analysis and facilitate data sharing among P01 projects and cores. The Core co-PIs and their laboratories are highly proficient in all the proposed techniques and assays, with a combined 200 and more publications. Whenever possible, the GBC will use the analytical pipeline used at Weill Cornell’s Institute for Precision Medicine for its CLIA/CLEP assays.

抽象的 基因组学和生物信息学核心（GBC）将为项目提供集中的设施、设备和 全外显子组测序、转录组测序、单细胞 RNAseq、血浆 DNA 的专业知识 测序、ATAC-seq 和 ChIP-seq 所有研究项目都将使用 GBC GBC 将由两位共同领导。 具有互补专业知识的 PI Elemento 博士和 Mason 博士将提供四种主要类型的服务。 服务：1）临床样本和细胞的集成全外显子组测序和转录组测序 线，2) 使用 ERRBS、ChIPseq 和 ATAC 对临床和研究样本进行综合表观基因组分析- seq，3) 单细胞分析和血浆 DNA 监测，4) 进行综合分析并促进 P01 项目和核心之间的数据共享 核心联合 PI 及其实验室在所有方面都非常精通。 所提出的技术和检测方法，以及 200 多篇出版物。 GBC 将使用威尔康奈尔精准医学研究所使用的分析流程进行 CLIA/CLEP 化验。