Small Animal Ocular Imaging Core

小动物眼部成像核心

基本信息

批准号：
10004633
负责人：
Edward N Pugh
金额：
$ 14.07万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-07-01 至 2024-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10004633
关键词：
Angiography Animals Anterior Cell physiology Cells Cellular Structures Collaborations Communication Consultations Core Grant Creativeness Custom Data Data Set Disease Equipment Experimental Designs Faculty Fluorescence Fluorescence Angiography Funding Fundus Fundus photography Future Goals Image Image Analysis Imaging Device Individual Laboratory Personnel Lasers Longitudinal Studies Mus Neurons Ophthalmoscopy Optical Coherence Tomography Phase Procedures Productivity Research Research Design Research Personnel Resolution Resources Retina Rodent Model Scanning Services Structure of retinal pigment epithelium Technology Vision research adaptive optics adaptive optics scanning laser ophthalmoscopy cost in vivo instrumentation laboratory experience ocular imaging recruit vision science

Angiography Animals Anterior Cell physiology Cells Cellular Structures Collaborations Communication Consultations Core Grant Creativeness Custom Data Data Set Disease Equipment Experimental Designs Faculty Fluorescence Fluorescence Angiography Funding Fundus Fundus photography Future Goals Image Image Analysis Imaging Device Individual Laboratory Personnel Lasers Longitudinal Studies Mus Neurons Ophthalmoscopy Optical Coherence Tomography Phase Procedures Productivity Research Research Design Research Personnel Resolution Resources Retina Rodent Model Scanning Services Structure of retinal pigment epithelium Technology Vision research adaptive optics adaptive optics scanning laser ophthalmoscopy cost in vivo instrumentation laboratory experience ocular imaging recruit vision science

展开

项目摘要

SUMMARY – SMALL ANIMAL OCULAR IMAGING CORE The Small Animal Ocular Imaging Core (SAOIC) provides a wide and growing array of in vivo ocular imaging services to investigators who employ rodent models of ocular function and disease. The technological services include widefield Micron IVTM fundus camera video imaging (fluorescence and reflectance, with laser damage capability); cellular-level resolution Optical Coherence Tomography (OCT) and Scanning Laser Ophthalmoscopy (SLO); Adaptive Optics (AO) versions of both (AO-OCT, AO-SLO); combined OCT/SLO; and polarization- selective OCT. An especially noteworthy feature of the instrumentation is its ability to repeatedly image the same retinal locus — and even the same cells — in individual mice over months. The SAOIC Director and Managing Director provide experimental design consultation and tutorial sessions describing the capabilities of the instrumentation to potential investigators. Because the equipment is customized and relatively difficult to operate, and the datasets collected very large, the SAOIC staff also provide image analysis services when needed. Finally, the staff also trains the laboratory personnel of Core participating investigators to use key imaging tools (including the Micron IVTM camera, and the widefield OCT/SLO) when long-term studies are undertaken.

摘要 - 小动物眼成像核心小型动物眼成像核（Saoic）提供了一系列宽阔而增长的体内眼成像为采用眼功能和疾病的啮齿动物模型的研究人员提供服务。技术服务包括宽阔的Micron IVTM底面相机视频成像（荧光和反射率，带有激光损伤容量）;细胞级分辨率光学相干断层扫描（OCT）和扫描激光眼镜检查（SLO）;同时（AO-OCT，AO-SLO）的自适应光学器件（AO）版本；联合OCT/SLO；和极化 - 选择性十月。乐器的一个特别值得注意的特征是它可以反复对其进行相同的形象在几个月内，单个小鼠的视网膜基因座 - 甚至相同的细胞。 SAOIC董事和管理主任提供实验设计咨询和教程会议，描述仪器向潜在的调查员。由于设备是定制的，并且相对难以操作，所以数据集收集了很大，SAOIC员工还需要在需要时提供图像分析服务。最后，工作人员还培训核心参与调查人员的实验室人员使用关键成像工具（包括Micron IVTM摄像机和Widefield OCT/SLO）进行长期研究。