PHENOTYPING THE MOUSE VISUAL SYSTEM

小鼠视觉系统表型分析

• 批准号: 6525050
• 负责人: Edward N Pugh
• 金额: $ 24.14万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-07 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6525050
• 关键词: computer program /software; cone cell; data collection methodology /evaluation; electroretinography; gene expression; gene mutation; genetically modified animals; laboratory mouse; light microscopy; phenotype; pupillography; retina; retinal adaptation; retinal bipolar neuron; rhodopsin; rod cell; statistics /biometry; vision; visual photoreceptor; visual photosensitivity

DESCRIPTION: (Adapted from applicant's abstract) The ERG is the method of choice for rapid phenotyping of mice with mutations in retina-related genes. This is due to its ease of use, its inherently quantitative character, and its capacity to report alterations in many functionally important features of specific retinal cell types. These cells include: rods, the dominant cell type in the retina; cones, the basis of daytime-vision; and secondary neurons with which rods and cones communicate their respective signals. Building on previous work by the investigators, the proposed research will perfect rapid protocols for quantitative phenotyping of mice with ERG components attributable to these cell types. This will include production of light stimulation standards and standard values for each measurable ERG parameter for the most widely used wild type (WT) mouse strains. Knowledge of the pupillary response is essential for quantifying retinal stimulation in most conditions, and the pupillary response provides intrinsically quantitative input/output information about the integrity of a well understood visual system circuit. Illumination rearing conditions have profound effects on murine retinal health and function in electroretinography. Therefore, pupillometry and retinal histology will be used to quantify the effects of rearing illumination history and to chart the natural developmental course of the phenotyping parameters of WT mice from 2 weeks to 1 year of age. The research program will also develop a computer-controlled, unified ERG/pupillometry apparatus, which will incorporate all necessary features for mass-phenotyping. This proposal will also address data collection, storage, analysis, dissemination, and archiving.

描述：（改编自申请人的摘要） ERG是用突变中的小鼠快速表型的选择方法 与视网膜相关的基因。这是由于它的易用性，它本质上是 定量特征及其报告许多改变的能力 特定视网膜细胞类型的功能重要特征。这些细胞 包括：杆，视网膜中的主要细胞类型；锥，基础 白天视频；和杆和锥体交流的次级神经元 他们各自的信号。在调查人员以前的工作的基础上， 拟议的研究将完善用于定量表型的快速方案 具有归因于这些细胞类型的ERG成分的小鼠。这将包括 生产光刺激标准和每个标准值 最广泛使用的野生型（WT）小鼠应变的可测量ERG参数。 对瞳孔反应的了解对于量化视网膜至关重要 在大多数情况下刺激，瞳孔反应提供 关于A的完整性的本质定量输入/输出信息 众所周知的视觉系统电路。照明饲养条件有 对视网膜造影的鼠视网膜健康和功能的深远影响。 因此，瞳孔测定法和视网膜组织学将用于量化 饲养照明历史的影响并绘制自然发育 WT小鼠的表型参数的过程从2周到1岁。 该研究计划还将开发一个由计算机控制的，统一的 ERG/Pupillemetry设备，它将包含所有必要的功能 质量型。该建议还将解决数据收集，存储， 分析，传播和存档。