Bio CaRGOS: Capture and release gels for optimized storage of Cancer Biospecimens

Bio CaRGOS：捕获和释放凝胶以优化癌症生物样本的存储

• 批准号: 10025904
• 负责人: Gautam Gupta
• 金额: $ 54.94万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10025904
• 关键词: Achievement; Address; Albumins; Amylases; Antigens; Area; Biological Markers; Biomass; Buffers; Cancer Model; Cell Line; Chemicals; Clinical; Colloids; Complex; Cryopreservation; DNA; Detection; Diagnosis; Disease; Early Diagnosis; Electrolytes; Equipment; Escherichia coli; Experimental Designs; Fibrinogen; Formulation; Freezing; Gel; Globulins; Glucose; Goals; Hemoglobin; Human; Hydrolysis; Immunoassay; Individual; Ionic Strengths; Knowledge; Malignant Neoplasms; Malignant neoplasm of pancreas; Measures; Methodology; Methods; Modeling; Monitor; Nature; Oxides; Patient Monitoring; Performance; Pharmaceutical Preparations; Pharmacologic Substance; Phosphate Buffer; Physiological; Plasma; Plasma Proteins; Play; Polymerase Chain Reaction; Process; Property; Proteins; RNA; Recovery; Recurrence; Reproducibility; Research; Retrieval; Reverse Transcription; Role; Saline; Sampling; Silanes; Silicon Dioxide; Structure; Techniques; Technology; Temperature; Testing; Time; Uric Acid; Virus; anticancer research; aqueous; base; biomaterial compatibility; cancer biomarkers; clinical diagnostics; cost; digital; high reward; high risk; innovation; insight; interest; macromolecule; microwave electromagnetic radiation; preservation; solute; specific biomarkers; user-friendly

Biomarkers play a central role in the detection, diagnosis, management and monitoring of patients with cancer. Factors such as analytical sensitivity and reproducibility are critical to the clinical utility of plasma based biomarkers in the setting of longitudinal surveillance and early detection of disease and or recurrence. Currently, the state of preservation of biospecimens is accomplished primarily using sub freezer techniques and it is cost prohibitive and certainly not practical in most scenarios. There is clearly a dearth of techniques that can preserve the biospecimens at room temperature for long periods of time. Therefore, there is a critical unmet clinical need for storing a plethora of biospecimens acquired routinely. Here, we propose to demonstrate stability and integrity of Pancreatic Cancer Specific biomarkers (CA-A19, miRNA21, and ctDNA) in buffers as well as plasma for extended periods of time at room temperature. There are several hurdles that must be overcome to realize the stabilization of biomarkers in plasma directly, due to its inherent complex nature. The proposed technology is based upon our innovation in developing CaRGOS: Capture and Release Gels for Optimized storage of Biospecimens. The technical impact of the proposed methodology is achievement of instantaneous one-step aqueous biospecimen preservative method for cancer research and clinical samples of interest. The proposed technology is extremely simple, user friendly, eliminates several complex processes, and more importantly requires no substantial equipment. The key scientific impact includes our fundamental insight into the interactions of the biomarkers and other proteins/solute with silica precursors. Based on this fundamental principle, the methodology can be easily expanded to other research areas including clinical samples, antigens, bio-pharmaceuticals, drugs, viruses and cell lines. If successful, the innovative proposed research will result in a paradigm shift in storage and preservation of Cancer Biospecimens in aqueous formulations that current techniques are unable to achieve.

生物标志物在患者的检测，诊断，管理和监测中起着核心作用 癌症。分析灵敏度和可重复性等因素对临床至关重要 基于血浆的生物标志物在纵向监视和早期检测中的效用 疾病和或复发。目前，实现了生物测量的状态 主要使用子冰柜技术，它的成本高昂，在大多数方面肯定不实用 方案。显然，缺乏能够保存在房间的生物测量的技术 温度长时间。因此，存在至关重要的临床需求 经常收购了很多生物素。 在这里，我们建议证明胰腺癌特异性的稳定性和完整性 生物标志物（CA-A19，miRNA21和ctDNA）在缓冲液以及血浆中长期长时间 室温的时间。有几个障碍必须克服才能实现 由于其固有的复杂性，生物标志物在血浆中的稳定。提议 技术是基于我们在开发货物方面的创新：捕获和释放凝胶 优化的生物测量存储。所提出方法的技术影响是 实现抗癌症的瞬时一步水性生物测量方法 研究和感兴趣的临床样本。提出的技术非常简单，用户 友好，消除了几个复杂的过程，更重要的是不需要实质性 设备。关键的科学影响包括我们对互动的基本见解 具有二氧化硅前体的生物标志物和其他蛋白质/溶质。基于这个基本原则， 该方法可以轻松扩展到其他研究领域，包括临床样本， 抗原，生物药物，药物，病毒和细胞系。如果成功，创新 拟议的研究将导致癌症存储和保存的范式转移 当前技术无法实现的水性配方中的生物测量。