Aptamer Reagents for Immunologic Analysis of Underrepresentative Mammalian Models of Tuberculosis

用于代表性不足的哺乳动物结核病模型免疫学分析的适体试剂

• 批准号: 10018161
• 负责人: JACKSON WILLIAM
• 金额: $ 60万
• 依托单位: BASE PAIR BIOTECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2022-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10018161
• 关键词: Address; Affinity; Animal Model; Antibodies; Archives; Base Pairing; Binding; Bioinformatics; Biological Assay; Biotechnology; Cancer Immunology Science; Cavia; Cells; Clinical Trials; Cotton Rats; Coupled; Development; Discrimination; Disease; Disease Progression; Enzyme-Linked Immunosorbent Assay; Ferrets; Goals; Human; Immune; Immune response; Immunologic Markers; Immunological Models; Immunologics; Infection; Interferometry; Legal patent; Ligands; Measurement; Modeling; Mycobacterium tuberculosis; Oryctolagus cuniculus; Phase; Process; Proteins; Reagent; Recombinants; Research; Reticulocytes; Sampling; Serum; Slide; Specific qualifier value; Specificity; Stains; System; Technology; Testing; Texas; Time; Tissues; Tuberculosis; Universities; Validation; aptamer; cDNA Expression; experience; novel; off-patent; professor; programs; screening; synthetic peptide; tool

This goal of this program is to address the limited availability of reagents (antibodies, proteins, ligands) for the identification and discrimination of immune cells and the characterization of immune responses in nonmammalian models and mammalian models for which immunologic reagents are limited (e.g. guinea pig, ferret, cotton rat). For example, the progression of disease that follows infection of guinea pigs with Mycobacterium tuberculosis displays many features of human TB. While this model has been used for more than 100 years as a research tool to understand and describe disease mechanisms, immunologic analyses are constrained by the limited availability of immunological reagents specific for the guinea pig. Another example is the ferret model where immunologic studies also have been limited by the lack of immunological reagents. In this project, Base Pair Biotechnologies will use its patented process for highly multiplexed aptamer selection to rapidly generate at least 60 new affinity reagents in Phase I to important immune markers in guinea pigs and ferrets. Our expert consultant in TB animal models will aid in target selection along with our staff with extensive bioinformatics experience. These new reagents will be characterized for their binding constants, specificity, and demonstrated in ELISA-like assays, and ELISpot. In Phase II, we will use the same process to generate at least 100 more reagents and employed in additional applications relevant to cancer immunology studies and clinical trials.

该程序的这个目标是解决针对免疫细胞鉴定和歧视的试剂（抗体，蛋白质，配体）的有限可用性，以及在非乳腺模型和免疫学试剂的哺乳动物模型中的免疫反应的表征，免疫学试剂有限（例如豚鼠，雪貂，棉花比）。例如，在结核分枝杆菌感染豚鼠后，疾病的进展表现出人类结核病的许多特征。尽管该模型已被用作了解和描述疾病机制的研究工具已有100多年的历史，但免疫学分析受到针对豚鼠特异性免疫试剂的有限可用性的限制。另一个例子是雪貂模型，该模型也因缺乏免疫学试剂而受到限制。在这个项目中，基对生物技术将使用其专利过程来进行高度多重的适体选择，以迅速在I期中至少生成60种新的亲和力试剂，以对豚鼠和雪貂的重要免疫标记。我们的结核病动物模型专家顾问将有助于与我们拥有丰富生物信息学经验的员工一起进行目标选择。这些新试剂将以其结合常数，特异性和ELISPOT的结合常数为特征。在第二阶段，我们将使用相同的过程产生至少100种试剂，并在与癌症免疫学研究和临床试验有关的其他应用中使用。